Method for preparing biological active small peptide with endogenesis enzyme

A technology of biological activity and production process, applied in animal protein processing, animal feed, food science, etc., can solve the problems of high cost, long time for enzymatic hydrolysis of small peptides, and difficulty in industrialized large-scale production, so as to achieve cost saving and large-scale production. The effect of easy production and simplified production process

Active Publication Date: 2009-01-14
JIANGSU TIANFULAI GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] At present, there are many methods for the preparation of bioactive peptides, the most common ones are enzymatic hydrolysis and microbial methods, but due to the high cost of external biochemical enzymes in the enzymatic hydrolysis method, the preparation of small peptides by the microbiological method takes too long to enzymatically hydrolyze, and the cost is high, so it is difficult to achieve industrialized production

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  • Method for preparing biological active small peptide with endogenesis enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. A production process for preparing biologically active small peptides with endogenous enzymes, the steps are as follows:

[0029] (1) Select marine low-value small miscellaneous fish or aquatic product processing scraps as raw materials. The raw materials are washed and crushed, and then water at 2°C is added to the raw materials at a weight ratio of 1:2 and put into a homogenizer or a homogenizer. Slurry in the middle to get fish paste material;

[0030] (2) Place the fish paste material in the enzymatic hydrolysis equipment, then add calcium chloride therein so that the concentration of the calcium chloride solution in the fish paste material is 0.02mol / L, and shake the fish paste material evenly under refrigeration; Then the fish paste material is heated up and the temperature is controlled at 53°C, the pH is adjusted to 6.8 with a hydrochloric acid solution with a concentration of 1.0mol / L and a sodium hydroxide solution with a concentration of 1.0mol / L,...

Embodiment 2

[0032] Example 2. A production process for preparing biologically active small peptides with endogenous enzymes, the steps are as follows:

[0033] (1) Select marine low-value small miscellaneous fish or aquatic product processing scraps as raw materials. The raw materials are washed and crushed, and then water at 6°C is added to the raw materials at a weight ratio of 1:3 and put into a homogenizer or a homogenizer. Slurry in the middle to get fish paste material;

[0034] (2) Place the fish paste material in the enzymatic hydrolysis equipment, then add calcium chloride therein so that the concentration of the calcium chloride solution in the fish paste material is 0.03mol / L, and shake the fish paste material evenly under refrigeration; Then the fish paste material is heated up and the temperature is controlled at 57°C, the pH is adjusted to 7.2 with a hydrochloric acid solution with a concentration of 2.0mol / L and a sodium hydroxide solution with a concentration of 2.0mol / L,...

Embodiment 3

[0036] Example 3. A production process for preparing biologically active small peptides with endogenous enzymes, the steps are as follows:

[0037] (1) Select marine low-value small miscellaneous fish or aquatic product processing waste as raw materials. The raw materials are washed and crushed, and then water at 4°C is added to the raw materials at a weight ratio of 1:2.5 and put into a homogenizer or a homogenizer. Slurry in the middle to get fish paste material;

[0038] (2) The fish paste material is placed in the enzymatic hydrolysis equipment, and then calcium chloride is added therein so that the concentration of the calcium chloride solution in the fish paste material is 0.025mol / L, and the fish paste material is shaken evenly under refrigeration; Then the fish paste material is heated up and the temperature is controlled at 55°C, the pH is adjusted to 7 with a hydrochloric acid solution with a concentration of 1.5mol / L and a sodium hydroxide solution with a concentra...

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Abstract

The invention relates to a production process for preparing biological active small peptide with endogenous enzyme, which is characterized in that low value freshwater fish or aquatic product processing leftover is selected as raw material; the raw material is pulped after being cleaned, and water is added to prepare fish cream; the fish cream is put into a zymohydrolysis device, calcium chloride is added in, and the fish cream is heated to raise and control the temperature to be 53 DEG C to 57 DEG C, the ph value is regulated, zymohydrolysis is conducted, to obtain enzymolysis product; enzyme killing and sterilization are conducted to the enzymolysis product, impurities in enzyme- hydrolyzed solution are filtered, to obtain coarsely filtered enzyme- hydrolyzed solution; the enzyme- hydrolyzed solution is concentrated, to obtain liquid or paste biological active small peptide. The production process pursues the best autologous enzymolysis conditions with the endogenous enzyme system in the mechanism of low value freshwater fish, not only greatly saves the cost, but also simplifies the production process of the biological active small peptide, so that the mass production is easy to be realized.

Description

technical field [0001] The invention relates to a production process of an animal feed additive, in particular to a production process for preparing biologically active small peptides with endogenous enzymes. Background technique [0002] Small peptides are intermediate products in the process of degrading protein into amino acids in animals. They are important nutrients for animals and one of the important ways of protein nutrition absorption and metabolism except amino acids. Small peptides and free amino acids have independent absorption mechanisms. They do not interfere with each other, and help the absorption of amino acids and speed up protein synthesis. Adding peptide products to animal diets can increase the utilization rate of amino acids, reduce the occurrence of diseases, give full play to its production performance and improve economic benefits. [0003] At present, there are many methods for the preparation of bioactive peptides, the most common ones are enzyma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/10A23J3/04A23J3/34A23K20/147
Inventor 陈忆凤
Owner JIANGSU TIANFULAI GROUP
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