Tulipa fosteriana flavonol synthase TfFLS protein and coding gene thereof

A flavonol synthase, synthase technology, applied in genetic engineering, plant gene improvement, enzymes and other directions, can solve the problem of lack of coding gene sequence and so on
CN103756982AInactive Publication Date: 2014-04-30SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2014-04-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a Tulipa fosteriana flavonol synthase TfFLS protein and a coding gene thereof. The protein is a protein composed by an amino acid sequence shown in SEQ ID No. 2 or a protein composed of an amino acid sequence obtained through replacement, deletion or addition of one or more amino acids of the amino acid sequence shown in the SEQ ID No. 2 and having the activity of Tulipa fosteriana flavonol synthase. The invention further provides a nucleic acid sequence used for coding the protein, and the nucleic acid sequence is shown in SEQ ID No. 1. The Tulipa fosteriana flavonol synthase TfFLS gene provided by the invention expresses a recombinant flavonol synthase protein in Escherichia coli cells and enables dihydrokaempferol to produce kaempferol, which proves that the gene has the functions of flavonol synthase. With the TfFLS gene, substances, receptors, inhibitors, antagonists or the like interacting with TfFLS can be screened by using a variety of conventional screening methods.
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Description

technical field

[0001] The invention belongs to the field of plant molecular biology, and relates to a key enzyme in the synthetic pathway of tulip flavonoids and its coding gene, in particular to a tulip flavonol synthase TfFLS protein and its coding gene. Background technique

[0002] Flavonoids are a class of polyphenolic compounds that widely exist in nature. According to different modifications to the central C ring, they can be divided into major categories such as flavonoids, isoflavones, flavanones, flavonols, flavanols, and anthocyanins. Among them, flavonoid alcohols are the most common, accounting for about 1 / 3 of the total flavonoids. Flavonols are widely distributed in a variety of plants. They are inhibitors of several kinases related to intracellular signal transduction and cell transformation, and have a variety of biological activities, because they can selectively block intracellular signal transduction pathways , so flavonoids are considered as potential ...

Claims

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