Kit and method for detecting activity of sucrose phosphate synthase

A phosphate synthase and activity determination technology, applied in the field of life sciences, can solve the problems of cumbersome operation process, inaccurate measurement results, large amount of reagents added, etc., achieve accurate measurement results, reduce the types of reagents, and reduce the cost of reagents
CN105506061AInactive Publication Date: 2016-04-20SUZHOU COMIN BIOTECH
3 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU COMIN BIOTECH
Publication Date
2016-04-20
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
Patent Text Reader

Abstract

The invention discloses a kit and method for detecting the activity of sucrose phosphate synthase. The kit comprises an extracting solution prepared from Tris-HCl, magnesium chloride, EDTA and 2-mercaptoethanol, a reagent I prepared from Tris-HCl, magnesium chloride, 6-phosphofructose and UDPG, a reagent II prepared by dissolving saccharose in distilled water, a reagent III prepared by dissolving sodium hydroxide in distilled water, a reagent IV prepared by dissolving hydrochloric acid in distilled water and a reagent V prepared by dissolving resorcinol in ethanol and distilled water. According to the method, the influence of saccharose in a sample can be eliminated, so that the detection result is accurate, and the kit is suitable for detecting the activity of sucrose phosphate synthase in various samples. According to the kit and method, the number of reagent types can be greatly reduced, the operation can be simple and convenient, the total volume of the reaction is only 300mu L, the concentration of UDPG can be reduced on the base of not influencing the accuracy, and the reagent cost can be greatly reduced.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of life sciences and relates to a kit, in particular to a kit for measuring the activity of sucrose phosphate synthase and a method thereof. Background technique

[0002] Sucrose is not only an important photosynthetic product, but also the main substance transported in plants and one of the storage forms of carbohydrates. Sucrose phosphate synthase (SPS) accepts fructose-6-phosphate, and the formed sucrose phosphate is converted into sucrose under the action of sucrose phosphatase. Generally, the sucrose phosphate synthase-sucrose phosphatase system is regarded as the main pathway of sucrose synthesis.

[0003] The currently available method for detecting the activity of sucrose phosphate synthase is the resorcinol colorimetric method. The principle is that sucrose phosphate synthase catalyzes fructose-6-phosphate to form sucrose phosphate in the sample, and the depth of the color change presented by the reaction b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More