Method for inducing nematode-trapping fungi to synchronously produce trapping organs

A technology for preying on nematode fungi and predatory organs, applied in the field of applied microbiology, can solve the problems of asynchronous controllability of predatory organs, weak controllability, limited number of predatory organs, etc., and achieves simple and easy induction method, strong controllability, high controllability, etc. good repeatability

Inactive Publication Date: 2010-06-09
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods of inducing predatory organs have the disadvantages of limited number of predatory organs, asynchronous production of predatory organs, and poor controllability.
[0004] Through document search, do not find the public report of the same document as the content of the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Induction of predatory organs of the nematode-predating fungus Arthrobush oligosporum hyphae

[0034] Nematode extracts were prepared according to the method described above. Arthropodium oligospora was inoculated in PDA liquid medium, cultured at 26° C. and 160 rpm for 5 or 6 days, and mycelia were collected by filtration. The mycelium is washed with sterile deionized water 3 or 4 times to remove the medium components, and an appropriate amount of sterile deionized water is used to suspend the mycelia for the induction of three-dimensional bacterial nets.

[0035] Suspend the collected fresh mycelium of Arthropodium oligosporum with sterile deionized water, place it in a glass plate, 40mL / dish: add nematode extract (1:10, v / v); add an equal amount of sterile deionized water to the control group (vegetative mycelia group); place the plate in a constant temperature incubator at 26°C and incubate for 24 or 36 or 48 hours. After 15 hours of induction of the hyp...

Embodiment 2

[0036] Example 2: Induction of Predatory Organs Formed by Conidia of the Nematode-predating Fungus Arthrobush oligosporum

[0037] The nematode extract was prepared according to the method described above. Arthropodium oligospora was inoculated with CMA solid medium and cultured in a constant temperature incubator at 26°C for 10 days to obtain a large number of spores. Rinse the hyphae of Arthropodium oligospora on the CMA plate with sterile water, transfer the liquid on the plate to a funnel equipped with sterile lens tissue to filter with a pipette, and collect the spores.

[0038]After suspending the collected Arthropodium oligospora spores with sterile deionized water, place them in a glass plate, 20mL / dish: add nematode extract (1:10, v / v) Add an equal amount of sterile deionized water to the control group (vegetative hyphae group); place the plate in a constant temperature incubator at 26° C. and incubate for 24 or 36 or 48 hours. After 12-15 hours of induction of Arth...

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PUM

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Abstract

The invention relates to a method for inducing nematode-trapping fungi to synchronously produce trapping organs, which belongs to the field of applied microbiology. The method comprises the steps of culture of nematodes, preparation of a nematode extract, culture of nematode-trapping fungi and induction of trapping organs. The method can utilize the nematode extract to induce the nematode-trapping fungi to synchronously produce a large number of trapping organs (160 trapping organs/mg mycelium) by culturing the nematodes (Caenorhabditis elegans) and preparing the nematode extract by utilizing ultrasonic waves for crushing and centrifugalizing. The method has the advantages of simple and easy operation and good repeatability, and can obtain a large number of synchronous three-dimensional fungal nets, thereby providing good experimental materials for further research of the molecular mechanism of forming the trapping organs of the nematode-trapping fungi.

Description

Technical field: [0001] The invention relates to a method for inducing predation nematode fungi to synchronously produce predation organs, belonging to the field of applied microbiology. Background technique: [0002] Plant parasitic nematodes are one of the important diseases of plants, and the economic losses of crops caused by plant parasitic nematodes reach 78 billion US dollars every year in the world (Barker, 1998). In my country, nematodes harm almost all economic crops such as tobacco, flowers, vegetables, cotton, soybeans, peanuts, wheat, rice, forest trees, and traditional Chinese medicinal materials, and become one of the important limiting factors in agricultural production. At present, the control of nematode diseases is still dominated by chemical control, but due to the impact of chemical pesticides on the ecological environment and the increase in the resistance of parasitic nematodes, its application is gradually limited. Therefore, the use of natural enemie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P5/00C12R1/645
Inventor 杨金奎张克勤米其利梁连铭
Owner YUNNAN UNIV
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