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Gene adjusting and controlling rice tillering angle and its coded protein and use

A rice and angle technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of rice yield reduction, plant growth cramped, group photosynthetic efficiency reduction, etc., and achieve the effect of wide application and market prospects

Inactive Publication Date: 2006-10-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tiller angle refers to the angle between the tiller and the main stem and other tillers (Xu Y B, McCouch S R, Shen Z T. Transgressive segregation of tiller angle in rice caused by complementary gene action. CropSci, 1998, 38: 12~19.) , too large a tiller angle not only wastes space, reduces the rice planting density per unit area and leads to a decrease in yield, but also is not conducive to the mechanized harvesting of rice; on the contrary, too small a tiller angle will make the growth of the plant too cramped, and the ventilation and light transmission will not be smooth , resulting in vicious competition among tillers, reduced photosynthetic efficiency of the population, high humidity and easy disease

Method used

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  • Gene adjusting and controlling rice tillering angle and its coded protein and use
  • Gene adjusting and controlling rice tillering angle and its coded protein and use
  • Gene adjusting and controlling rice tillering angle and its coded protein and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1. Obtaining the transcription factor gene compact1 that regulates rice tillering angle

[0044] Rice varieties: Asominori, IR24, Dongxiang wild rice, Yuanjiang wild rice, Teqing, 9311, Guichao 2, E32, Nipponbare, Qiuguang, Shuiyuan 349 (all from the Seed Bank of the Rice Group, College of Agronomy and Biotechnology, China Agricultural University) .

[0045] Using Japonica rice Asominori as the donor and Indica rice IR24 as the recipient, a high-generation backcross population (BC3F4) was constructed, from which a compact plant type line with a small tiller angle was found. One of the plants was selected to be compact and only compared with the tiller angle phenotype. There is a difference in IR24 line IL55 (The comparison chart of the compact plant type IL55 and the plant type loose line IR24 is shown in figure 1 ), and crossed it with IR24 to construct an F2 segregating population containing 12,000 individual plants. In this F2 population, the ratio of individual...

Embodiment 2

[0046] Example 2. Obtaining of com1 transgenic rice and its PCR detection

[0047] 1. Construction of com1 plant expression vector

[0048] Design primers based on the full-length cDNA sequence of com1 of IR24, and introduce restriction endonuclease Sma I and Spe I recognition sites and protective bases at both ends of the primers. The primer sequences are as follows:

[0049] CDS-P-ATG: 5’- TGCCCCGGG CGTACTGTCTGGCTTTCTCTTCTGGT-3' (underlined bases are the recognition site of restriction endonuclease Sma I and protection bases);

[0050] CDS-P-TGA: 5’- CACTAGT AGGACTATTCTTCATCACTGGCG-3' (underlined bases are the recognition site of restriction endonuclease Spe I and protected bases)

[0051] Use TRIZOL reagent to extract RNA from the leaves of IR24 at the seedling stage (three leaves and one heart), use this RNA as a template, and use MMLV reverse transcriptase to perform reverse transcription to obtain cDNA, and then use this cDNA as a template with primers CDS-P-ATG Under the g...

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Abstract

The invention discloses a section of gene and its protein with controlling rice tillering angulations, and its application. The purpose is that providing a section of gene and its protein with controlling rice tillering angulations, and applying the gene and protein in controlling rice tillering angulations. The gene's cDNA has one of nucleotide sequences as follow: 1) the SEQ ID NO: 2 or SEQ ID NO: 4 DNA sequence in sequence table; 2) coding the DNA sequence of SEQ ID NO: 1 in the sequence table; 3) the nucleotide sequence that can hybrid with restrict DNA sequence of SEQ ID NO: 2 in the sequence table at high strict condition. The gene and its protein have important theory and operation significance in studying the molecular mechanism of rice tillering angulations and controlling rice plant type. The invention has extensive application and market outlook in agriculture field.

Description

Technical field [0001] The present invention relates to plant genes and their coded proteins and applications, in particular to a gene and its coded protein for regulating the tillering angle of rice and its application in regulating the tillering angle of rice. Background technique [0002] The plant type of rice controls the three-dimensional distribution of its different tissues on the plant. It can properly distribute and regulate the space, light, temperature, water, humidity, etc. of the rice field, and promote the normal development of plants. Rice plant type includes tiller angle, phyllodes, panicle type and plant height, among which tiller angle is an important factor affecting plant type. Tiller angle refers to the angle between the tiller and the main stem and other tillers (Xu YB, McCouch SR, Shen Z T. Transgressive segregation of tiller angle in rice caused by complementary gene action. CropSci, 1998, 38: 12-19.) Too large a tiller angle not only wastes space, reduce...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/63C12N15/82
Inventor 孙传清余柏胜林中伟李海霞李晓娇朱作峰付永彩李家洋
Owner CHINA AGRI UNIV
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