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Method for building virus hepatitis mice model

A viral hepatitis, mouse model technology, applied in animal husbandry and other directions, can solve the problems of lack of pathogen replication, different, etc., to achieve the effect of low cost, overcome limitations, and clear genetic background

Inactive Publication Date: 2003-12-31
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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  • Abstract
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  • Application Information

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Problems solved by technology

However, the model still suffers from the limitation of lack of pathogen replication in vivo, which is significantly different from the clinical situation

Method used

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Embodiment Construction

[0018] Below in conjunction with specific embodiment, the present invention is described in further detail:

[0019] The method for establishing a viral hepatitis mouse model proposed by the present invention includes the following steps:

[0020] 1) Preparation of different strains of mice: in groups of 12, select several groups of BALB / CJ pure female mice, several groups of C3H / HEJ pure female mice and several groups of A / J pure female mice , reared in animal breeding laboratory for reserve. The age of pure female mice in the above groups is preferably in the range of 6-8 weeks of gestational age, because the symptoms reflected by the mice in this age range after infection with hepatitis virus are very stable, and it is easy to simulate the infection of human beings. The optimal age of the selected mice should be 8 weeks gestational age. The animal grade of the pure female mice in the above groups should be clean grade, which can prevent the interference and influence of o...

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Abstract

The present invention is the method of establishing mouse model of viral hepatitis. The method includes selection of mouse hepatitis virus-III (MHV-3) of 100-1000 pFu, injecting infection of variousvariety mice of BALB / CJ, C3H / HEJ and A / J mice, and observing the survival rate and liver tissue change. Via the said process, three kinds of viral hepatitis mouse model are established, of fulminanthepatitis, chronic hepatitis and symptom-free mouse separately. The establishment of the model provides powerful facilities for systematic research of viral hepatitis in genetic regulation, molecular mechanism and prevention and treatment.

Description

technical field [0001] The invention relates to a method for establishing a mouse model of viral hepatitis, which is used for simulating, researching and preventing and treating human viral hepatitis. Background technique [0002] Viral hepatitis, especially viral hepatitis B, is an important disease that endangers human health. According to preliminary estimates, there are about 400 to 500 million carriers of hepatitis B virus (HBV) in the world. my country is a high-incidence area of ​​hepatitis B virus infection. There are more than 50,000 cases of fulminant hepatitis caused by viruses every year in the world, and the mortality rate is as high as 80-90%. The molecular basis of hepatocyte necrosis in viral hepatitis is still unclear, and there is no established effective treatment method. Therefore, in-depth research on the molecular immune mechanism of viral hepatitis hepatocyte necrosis can provide a theoretical basis for predicting the disease ev...

Claims

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Application Information

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IPC IPC(8): A01K67/027
Inventor 宁琴罗小平严伟明郝连杰
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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