Method for identifying resistance of sclerotinia by inoculating in-vitro stalk of plant

A sclerotinia and stem technology, which is applied in the field of plant disease and insect resistance identification, can solve the problems that leaf resistance cannot fully reflect stem resistance, field inoculation identification fails, and inoculation identification is unstable, and achieves stable inoculation identification, The effect of short cycle and simple method

Inactive Publication Date: 2012-12-05
SOUTHWEST UNIVERSITY
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Problems solved by technology

Although these methods are simple to operate, the resistance of leaves cannot fully reflect the resistance of stems.
After Sclerotinia infects the stems, it will lead to a severe reduction in yield
For stem resistance identification, there are field stem toothpick inoculation method and stem filament block binding method. The two methods are simple and easy to imp

Method used

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  • Method for identifying resistance of sclerotinia by inoculating in-vitro stalk of plant
  • Method for identifying resistance of sclerotinia by inoculating in-vitro stalk of plant

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Embodiment 1

[0011] 1. Preparation of Inoculum

[0012] 1) The pathogenic bacteria were collected in the experimental field of Chongqing Rapeseed Engineering Technology Center. The purification process is as follows: the collected sclerotia were inoculated in potato dextrose medium (PDA), cultured in the dark at 22°C for 2 days, and the hyphae were picked from the edge and inserted into New PDA medium, after 2 days, transfer the hyphae on the edge again, and so on, until the hyphae grown in the medium do not contain other miscellaneous bacteria. The PDA medium is prepared as follows: 200g potatoes, 20g sucrose and 15g agar powder, and the volume is adjusted to 1L.

[0013] 2) Preparation of inoculum (PDA mycelium block) for in vitro experiments: take a small piece of the activated strain, inoculate it in the center of a sterile petri dish with PDA medium, and culture it at 22°C and 85% humidity for 2 After the mycelia evenly covered the surface of the culture medium, a hole puncher with a...

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Abstract

The invention relates to a method for identifying the resistance of sclerotinia by inoculating an in-vitro stalk of a plant. The method comprises the following steps of: cutting the stalk or branches of the plant down, and putting the stalk or the branches into closed environment in which temperature and humidity are constant to inoculate sclerotinia sclerotiorum; and recording the extension lengths of bacterial plaques to measure the strength of disease resistance of the material. Compared with the conventional method for identifying the resistance of the stalk with sclerotinia, the method has the advantages that the method is performed under the condition of controllable environment, the pathogenetic condition is consistent, and inoculating identification is stable; the method is simple, short in period and high in inoculating efficiency; and inoculated parts are flexible, and a single plant can be identified repeatedly, so the method is suitable for the large-scale inoculating identification of the material.

Description

technical field [0001] The invention belongs to a method for identifying resistance to plant diseases and insect pests, in particular to a method for identifying resistance to sclerotinia by inoculating isolated plant stems. Background technique [0002] Sclerotinia can harm more than 400 kinds of plants, such as rapeseed, and the production of rapeseed in my country is reduced by 10-80% every year due to the damage of sclerotinia. Breeding disease-resistant varieties is the most economical way to control Sclerotinia. A simple and accurate identification method for Sclerotinia resistance is the key to screening resistant materials. [0003] At present, Sclerotinia resistance identification includes leaf resistance identification and stem resistance identification. Leaf resistance identification includes isolated leaf inoculation, leaf axil inoculation, petiole inoculation, cotyledon inoculation, etc. Although these methods are simple to operate, the resistance of leaves ca...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12R1/645
Inventor 钱伟梅家琴丁一娟魏大勇
Owner SOUTHWEST UNIVERSITY
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