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Method for rapidly identifying resistance of colletotricum destructivum

A technology for anthrax and anthrax germs, applied in biological testing, material inspection, etc., can solve time-consuming and labor-intensive problems, and achieve the effects of simple inoculation methods, consistent disease conditions, and high inoculation efficiency

Inactive Publication Date: 2013-11-20
TOBACCO RES INST CHIN AGRI SCI ACAD +1
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Tobacco anthracnose resistance identification generally adopts manual inoculation identification (GB / T23224-2008), and it is time-consuming and labor-intensive due to the constraints of growth period and environmental conditions.

Method used

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  • Method for rapidly identifying resistance of colletotricum destructivum
  • Method for rapidly identifying resistance of colletotricum destructivum
  • Method for rapidly identifying resistance of colletotricum destructivum

Examples

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Embodiment 1

[0030] Embodiment 1. Tobacco anthrax virus toxin resistance identification

[0031] N. nudicaulis, N. debneyi with high resistance, N. paniculata with high resistance, N. paniculata with moderate resistance, and K326 with disease resistance in the field were used as the test varieties (refer to "Tobacco Diseases in China", No. 8 pages)

[0032] Tobacco seeds were provided by the Variety Resource Group of Tobacco Institute, Chinese Academy of Agricultural Sciences.

[0033] The tobacco plants tested were at the 4-5 leaf stage.

[0034] Anthrax strain name and source: Anthrax T1, isolated from Zhucheng, Shandong. Reference: "A Study on the Identification and Biological Characteristics of a Tobacco Anthracnose Pathogen" ("Plant Protection Technology Innovation and Modern Agriculture Construction", Chinese Plant Protection Society 2012 Academic Papers), P: 78-83

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PUM

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Abstract

The invention provides a method for rapidly identifying the resistance of colletotricum destructivum, which belongs to a plant disease resistance identification technology. The method is characterized by immersing leaf disks by utilizing colletotrichum toxins and balancing the resistance of a material through measuring and counting a scab expansion degree; compared with a traditional method for identifying the manual inoculation resistance of the anthracnose, the method provided by the invention is carried out under a manually-controlled stable condition and incidence conditions are consistent; the method is simple in operation process, short in identification period and high in inoculation efficiency; the method can simultaneously and respectively identify the resistance to different pathogenic bacteria by the same material, and growth and development and flowering and fructification of normal seedlings are not influenced; the aim of simply, rapidly and accurately identifying the resistance to the anthracnose by tobaccos and germplasms of the tobaccos is realized in a high throughput manner; the method has a very great application value on screening the resistance of the colletotricum destructivum.

Description

technical field [0001] The invention relates to a plant disease resistance identification technology, in particular to a method for quickly identifying tobacco anthracnose resistance. Background technique [0002] Tobacco anthracnose is caused by Colletotricum destructivum, which belongs to the fungi kingdom (Fungi), Deuteromycotina, Coelomycetes, Melanconiales, Anthracnose (Colletotricum). The mycelium of the fungus has branches and septa, and conidia discs grow on the subsea, in which there are dark brown bristles with septa; Club-shaped, bearing conidia. The conidia are long tube-shaped, blunt at both ends, single cell, colorless, with an oil ball at each end. The hosts are mainly vegetables of the nightshade family, such as potatoes, tomatoes, eggplants, tobacco leaves, etc., and they can also damage cucumbers, watermelons, pumpkins, and clover. [0003] Anthracnose is one of the main diseases of the middle and lower leaves of tobacco plants in the seedbed stage and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
Inventor 王静孔凡玉张成省田华冯超
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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