Jute anthracnose resistance identification method
A technology for identification of resistance and anthracnose, applied in biochemical equipment and methods, measurement/inspection of microorganisms, etc., can solve problems such as identification methods of resistance to jute anthracnose, and achieve conditions that are conducive to rapid infection and onset Consistent, easy-to-use results
Inactive Publication Date: 2015-11-25
FUJIAN AGRI & FORESTRY UNIV
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Abstract
The invention discloses a jute anthracnose resistance identification method, and belongs to the technical field of identification of plant disease resistance. The identification method comprises steps of strain culture, wound formation, mycelium inoculation, bagging moisture preservation and disease spot measurement, and specifically the method comprises the following steps: acquiring a jute anthracnose strain by virtue of a tissue isolation method, culturing and purifying the strain, and removing upper leaves of a to-be-detected jute plant from petiole bases by virtue of a blade so as to form a wound; then inoculating the cultured jute anthracnose strain on the wound, spraying water to the inoculated part, covering the inoculated part with a preservative bag and moisturizing for 2-3 days, removing the bag and continuing to infect for 15 days; and finally, determining the resistance strength of the jute plant by measuring the size of diseases spots. The method has the advantages of simple operation process, short identification cycle, high inoculation efficiency and the like; and the method is quite high in application value on anthracnose resistance identification and screening of jute germplasm resources.
Application Domain
Microbiological testing/measurement
Technology Topic
Plant disease resistanceBiology +5
Examples
- Experimental program(3)
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