Jute anthracnose resistance identification method

A technology for identification of resistance and anthracnose, applied in biochemical equipment and methods, measurement/inspection of microorganisms, etc., can solve problems such as identification methods of resistance to jute anthracnose, and achieve conditions that are conducive to rapid infection and onset Consistent, easy-to-use results

Inactive Publication Date: 2015-11-25

FUJIAN AGRI & FORESTRY UNIV

3 Cites 3 Cited by

AI-Extracted Technical Summary

Problems solved by technology

However, there are no reports or patents related to jute anth...

Abstract

The invention discloses a jute anthracnose resistance identification method, and belongs to the technical field of identification of plant disease resistance. The identification method comprises steps of strain culture, wound formation, mycelium inoculation, bagging moisture preservation and disease spot measurement, and specifically the method comprises the following steps: acquiring a jute anthracnose strain by virtue of a tissue isolation method, culturing and purifying the strain, and removing upper leaves of a to-be-detected jute plant from petiole bases by virtue of a blade so as to form a wound; then inoculating the cultured jute anthracnose strain on the wound, spraying water to the inoculated part, covering the inoculated part with a preservative bag and moisturizing for 2-3 days, removing the bag and continuing to infect for 15 days; and finally, determining the resistance strength of the jute plant by measuring the size of diseases spots. The method has the advantages of simple operation process, short identification cycle, high inoculation efficiency and the like; and the method is quite high in application value on anthracnose resistance identification and screening of jute germplasm resources.

Application Domain

Microbiological testing/measurement

Technology Topic

Plant disease resistanceBiology +5

Examples

Experimental program(3)

Example Embodiment

[0015] Example 1

[0016] A method for identification of jute anthracnose resistance, its specific operation is as follows:

[0017] 1) Strain culture: The strain of Bacillus anthracis jute was obtained by tissue separation method, cultivated and purified on PSA (potato sucrose agar) medium;

[0018] 2) Wound formation: at 6:00 in the morning, cut off the upper 3 leaves at the base of the petiole with a blade to form a neat wound;

[0019] 3) Mycelia inoculation: use a hole puncher with a pore size of 5 mm to cut the bacterial block on the cultivated and purified jute anthracnose strain, pick the bacterial block with an inoculation needle, make the mycelium directly contact the wound on the jute plant, and Press the bacterial block on the wound for inoculation, and wrap it with tinfoil;

[0020] 4) Moisturizing in bags: Spray water on the inoculated part with a watering can, and cover it with a fresh-keeping bag. If there are no water droplets or moisture in the bag, continue to spray water in the bag with a watering can to moisturize;

[0021] 5) Lesion measurement: remove the fresh-keeping bag after bagging for 3 days, and measure the size of the lesion on the jute plant after 15 days of continuous infection. mm 2 ) are 0-8, 8.1-16.0, 16.1-24.0, 24.1-32.0, 32.1-40.0, which are divided into 0, 1, 2, 3 and 4 grades in turn;

[0022] 6) Resistance evaluation: Calculate the jute anthracnose disease index according to the disease classification, and determine the resistance strength of jute plants according to the obtained disease index: high resistance (0), resistance (0.1-10.0), moderate resistance (10.1-30.0 ), middle sense (30.1-50.0), sense (50.1-60.0) and high sense (above 60.0);

[0023] The calculation method of jute anthracnose disease index is as follows:

[0024] Disease index = 100 × ∑ (number of lesions at all levels × representative value at each level)/(total number of surveys × representative value at the highest level).

Example Embodiment

[0025] Example 2

[0026] A method for identification of jute anthracnose resistance, its specific operation is as follows:

[0027] 1) Strain culture: The strain of Bacillus anthracis jute was obtained by tissue separation method, cultivated and purified on PSA (potato sucrose agar) medium;

[0028] 2) Wound formation: at 7:00 in the morning, cut off the upper 3 leaves at the base of the petiole with a blade to form a neat wound;

[0029] 3) Mycelia inoculation: use a hole puncher with a pore size of 5 mm to cut the bacterial block on the cultivated and purified jute anthracnose strain, pick the bacterial block with an inoculation needle, make the mycelium directly contact the wound on the jute plant, and Press the bacterial block on the wound for inoculation, and wrap it with tinfoil;

[0030] 4) Moisturizing in bags: Spray water on the inoculated part with a watering can, and cover it with a fresh-keeping bag. If there are no water droplets or moisture in the bag, continue to spray water in the bag with a watering can to moisturize;

[0031] 5) Lesion measurement: Remove the fresh-keeping bag after bagging for 2 days, and measure the size of the lesion on the jute plant after 15 days of continuous infection. mm 2 ) are 0-8, 8.1-16.0, 16.1-24.0, 24.1-32.0, 32.1-40.0, which are divided into 0, 1, 2, 3 and 4 grades in turn;

[0032] 6) Resistance evaluation: Calculate the jute anthracnose disease index according to the disease classification, and determine the resistance strength of jute plants according to the obtained disease index: high resistance (0), resistance (0.1-10.0), moderate resistance (10.1-30.0 ), middle sense (30.1-50.0), sense (50.1-60.0) and high sense (above 60.0);

[0033] The calculation method of jute anthracnose disease index is as follows:

[0034] Disease index = 100 × ∑ (number of lesions at all levels × representative value at each level)/(total number of surveys × representative value at the highest level).

Example Embodiment

[0035] Example 3

[0036] A method for identification of jute anthracnose resistance, its specific operation is as follows:

[0037] 1) Strain culture: The strain of Bacillus anthracis jute was obtained by tissue separation method, cultivated and purified on PSA (potato sucrose agar) medium;

[0038] 2) Wound formation: At 8:00 in the morning, cut off the upper 4 leaves at the base of the petiole with a blade to form a neat wound;

[0039] 3) Mycelia inoculation: use a hole puncher with a pore size of 5 mm to cut the bacterial block on the cultivated and purified jute anthracnose strain, pick the bacterial block with an inoculation needle, make the mycelium directly contact the wound on the jute plant, and Press the bacterial block on the wound for inoculation, and wrap it with tinfoil;

[0040]4) Moisturizing in bags: Spray water on the inoculated part with a watering can, and cover it with a fresh-keeping bag. If there are no water droplets or moisture in the bag, continue to spray water in the bag with a watering can to moisturize;

[0041] 5) Lesion measurement: remove the fresh-keeping bag after bagging for 3 days, and measure the size of the lesion on the jute plant after 15 days of continuous infection. mm 2 ) are 0-8, 8.1-16.0, 16.1-24.0, 24.1-32.0, 32.1-40.0, which are divided into 0, 1, 2, 3 and 4 grades in turn;

[0042] 6) Resistance evaluation: Calculate the jute anthracnose disease index according to the disease classification, and determine the resistance strength of jute plants according to the obtained disease index: high resistance (0), resistance (0.1-10.0), moderate resistance (10.1-30.0 ), middle sense (30.1-50.0), sense (50.1-60.0) and high sense (above 60.0);

[0043] The calculation method of jute anthracnose disease index is as follows:

[0044] Disease index = 100 × ∑ (number of lesions at all levels × representative value at each level)/(total number of surveys × representative value at the highest level).

[0045] 144 jute germplasms were identified for anthracnose resistance by the method described in Example 1, wherein 32 parts of disease-resistant germplasms were identified, 16 parts of susceptible germplasms, 59 parts of moderately resistant germplasms, and 37 parts of moderately sensitive germplasms , compared with the results of natural disease in the field, the coincidence rate was over 98%.

[0046] Compared with the existing acupuncture inoculation method, the method provided by the invention has the advantages of susceptibility, rapid onset, and easy identification; compared with the spore spray method, the method provided by the invention has the advantages of being basically not affected by climatic conditions and having high reliability. It has great application value in the identification and screening of anthracnose resistance in jute germplasm resources, and can provide a theoretical basis for the selection of new jute varieties resistant to anthracnose and QTL positioning.

PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.

Jute anthracnose resistance identification method

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

Similar technology patents

Recycled modified plastic production technology based on waste plastic woven bags

Broom convenient to clean

Log processing method and device

Microwave reaction synthesis method for endogenous metal matrix composite material

Control method and control system for water feed pump turbine