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Jute anthracnose resistance identification method

A technology for identification of resistance and anthracnose, applied in biochemical equipment and methods, measurement/inspection of microorganisms, etc., can solve problems such as identification methods of resistance to jute anthracnose, and achieve conditions that are conducive to rapid infection and onset Consistent, easy-to-use results

Inactive Publication Date: 2015-11-25
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports or patents related to jute anthracnose resistance identification methods at home and abroad.

Method used

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Examples

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Effect test

Embodiment 1

[0016] A method for identification of jute anthracnose resistance, its specific operation is as follows:

[0017] 1) Strain culture: The strain of Bacillus anthracis jute was obtained by tissue separation method, cultivated and purified on PSA (potato sucrose agar) medium;

[0018] 2) Wound formation: at 6:00 in the morning, cut off the upper 3 leaves at the base of the petiole with a blade to form a neat wound;

[0019] 3) Mycelia inoculation: use a hole puncher with a pore size of 5 mm to cut the bacterial block on the cultivated and purified jute anthracnose strain, pick the bacterial block with an inoculation needle, make the mycelium directly contact the wound on the jute plant, and Press the bacterial block on the wound for inoculation, and wrap it with tinfoil;

[0020] 4) Moisturizing in bags: Spray water on the inoculated part with a watering can, and cover it with a fresh-keeping bag. If there are no water droplets or moisture in the bag, continue to spray water in ...

Embodiment 2

[0026] A method for identification of jute anthracnose resistance, its specific operation is as follows:

[0027] 1) Strain culture: The strain of Bacillus anthracis jute was obtained by tissue separation method, cultivated and purified on PSA (potato sucrose agar) medium;

[0028] 2) Wound formation: at 7:00 in the morning, cut off the upper 3 leaves at the base of the petiole with a blade to form a neat wound;

[0029] 3) Mycelia inoculation: use a hole puncher with a pore size of 5 mm to cut the bacterial block on the cultivated and purified jute anthracnose strain, pick the bacterial block with an inoculation needle, make the mycelium directly contact the wound on the jute plant, and Press the bacterial block on the wound for inoculation, and wrap it with tinfoil;

[0030] 4) Moisturizing in bags: Spray water on the inoculated part with a watering can, and cover it with a fresh-keeping bag. If there are no water droplets or moisture in the bag, continue to spray water in ...

Embodiment 3

[0036] A method for identification of jute anthracnose resistance, its specific operation is as follows:

[0037] 1) Strain culture: The strain of Bacillus anthracis jute was obtained by tissue separation method, cultivated and purified on PSA (potato sucrose agar) medium;

[0038] 2) Wound formation: At 8:00 in the morning, cut off the upper 4 leaves at the base of the petiole with a blade to form a neat wound;

[0039] 3) Mycelia inoculation: use a hole puncher with a pore size of 5 mm to cut the bacterial block on the cultivated and purified jute anthracnose strain, pick the bacterial block with an inoculation needle, make the mycelium directly contact the wound on the jute plant, and Press the bacterial block on the wound for inoculation, and wrap it with tinfoil;

[0040]4) Moisturizing in bags: Spray water on the inoculated part with a watering can, and cover it with a fresh-keeping bag. If there are no water droplets or moisture in the bag, continue to spray water in t...

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Abstract

The invention discloses a jute anthracnose resistance identification method, and belongs to the technical field of identification of plant disease resistance. The identification method comprises steps of strain culture, wound formation, mycelium inoculation, bagging moisture preservation and disease spot measurement, and specifically the method comprises the following steps: acquiring a jute anthracnose strain by virtue of a tissue isolation method, culturing and purifying the strain, and removing upper leaves of a to-be-detected jute plant from petiole bases by virtue of a blade so as to form a wound; then inoculating the cultured jute anthracnose strain on the wound, spraying water to the inoculated part, covering the inoculated part with a preservative bag and moisturizing for 2-3 days, removing the bag and continuing to infect for 15 days; and finally, determining the resistance strength of the jute plant by measuring the size of diseases spots. The method has the advantages of simple operation process, short identification cycle, high inoculation efficiency and the like; and the method is quite high in application value on anthracnose resistance identification and screening of jute germplasm resources.

Description

technical field [0001] The invention belongs to the technical field of identification of plant disease resistance, in particular to a method for identification of jute anthracnose resistance. Background technique [0002] Jute belongs to the genus Jute (Tiliaceae) Corchorus ) is an annual plant and is the second largest fiber crop after cotton in the world. It is widely cultivated in India, Bangladesh, China, Thailand and other countries. In recent years, it has attracted much attention as an excellent raw material for papermaking, textile and biofuel. At the same time, jute fiber has excellent characteristics such as good heat dissipation, strong hygroscopicity, easy degradation, and high yield, which has attracted the attention of countries all over the world. [0003] With the continuous deepening of research, more and more uses of jute have been developed. In order to meet the growth of people's demand for natural fibers, the planting area of ​​jute is gradually increa...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
Inventor 陶爱芬祁建民高红徐建堂方平平林荔辉林培清吴建梅
Owner FUJIAN AGRI & FORESTRY UNIV
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