A method for identifying resistance to Sclerotinia sclerotiorum by plant in vitro stem inoculation

Abstract

The invention relates to a method for identifying the resistance of sclerotinia by inoculating an in-vitro stalk of a plant. The method comprises the following steps of: cutting the stalk or branches of the plant down, and putting the stalk or the branches into closed environment in which temperature and humidity are constant to inoculate sclerotinia sclerotiorum; and recording the extension lengths of bacterial plaques to measure the strength of disease resistance of the material. Compared with the conventional method for identifying the resistance of the stalk with sclerotinia, the method has the advantages that the method is performed under the condition of controllable environment, the pathogenetic condition is consistent, and inoculating identification is stable; the method is simple, short in period and high in inoculating efficiency; and inoculated parts are flexible, and a single plant can be identified repeatedly, so the method is suitable for the large-scale inoculating identification of the material.

Description

technical field [0001] The invention belongs to a method for identifying resistance to plant diseases and insect pests, in particular to a method for identifying resistance to sclerotinia by inoculating isolated plant stems. Background technique [0002] Sclerotinia can harm more than 400 kinds of plants, such as rapeseed, and the production of rapeseed in my country is reduced by 10-80% every year due to the damage of sclerotinia. Breeding disease-resistant varieties is the most economical way to control Sclerotinia. A simple and accurate identification method for Sclerotinia resistance is the key to screening resistant materials. [0003] At present, Sclerotinia resistance identification includes leaf resistance identification and stem resistance identification. Leaf resistance identification includes isolated leaf inoculation, leaf axil inoculation, petiole inoculation, cotyledon inoculation, etc. Although these methods are simple to operate, the resistance of leaves ca...

AI Technical Summary

Problems solved by technology

Although these methods are simple to operate, the resistance of leaves cannot fully reflect the resistance of stems.

After Sclerotinia infects the stems, it will lead to a severe reduction in yield

For stem resistance identification, there are field stem toothpick inoculation method and stem filament block binding method. The two methods are simple and easy to implement, and are suitable for large-scale inoculation identification, but they are easily affected by field environmental conditions, and inoculation identification is unstable.

For example, encountering dry and other climatic conditions that are not conducive to the occurrence of Sclerotinia sclerotiorum will lead to the failure of field inoculation identification

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