Bacillus subtilis CYY-25 and application thereof

A technology of Bacillus subtilis and CYY-25, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of complex pathogens and achieve the effect of promoting plant growth

Active Publication Date: 2016-02-03
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the pathogen of alfalfa root rot is complex,

Method used

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  • Bacillus subtilis CYY-25 and application thereof
  • Bacillus subtilis CYY-25 and application thereof
  • Bacillus subtilis CYY-25 and application thereof

Examples

Experimental program
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Embodiment Construction

[0027] The present invention will be further described in detail below in conjunction with specific drawings and embodiments, which are explanations rather than limitations of the present invention.

[0028] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples were purchased from conventional biochemical reagent stores unless otherwise specified. For the quantitative experiments in the following examples, the experiments were repeated more than three times, and the results were averaged.

[0029] 1. Isolation and identification of Bacillus subtilis CYY-25 (Bacillus subtilis)

[0030] 1. One. Isolation of Bacillus subtilis CYY-25 (Bacillus subtilis)

[0031] 1) Soil sample collection

[0032] The soil samples for the test were taken from the rhizosphere of alfalfa planted in the experimental field of Harbin Normal University, and the rhizosphere soil of the plant was ...

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Abstract

The invention discloses bacillus subtilis CYY-25 and belongs to the field of microorganisms. The bacillus subtilis CYY-25 is preserved with the preservation number CGMCC No.8995 in China General Microbiological Culture Collection Center and is capable of producing cellulase when salinity ranges from 0% to 15%. The bacillus subtilis CYY-25 is capable of synthetizing IAA and siderophore, dissolving sparingly soluble inorganic phosphorus, promoting plant growth and well inhibiting multiple kinds of plant pathogenic fungi such as fusarium oxysporum, pyricularia oryzae, sclerotinia sclerotiorum, fusarium moniliforme and sclerotinia sclerotiorum, is of great significance to prevention and control of alfalfa root rot and is suitable for being developed and used as an agricultural fungicide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of bacillus subtilis CYY-25 and an application thereof. Background technique [0002] Alfalfa (Medicagosativa L.) is the earliest and most widely cultivated high-quality perennial leguminous forage in the world. It has the advantages of high grass yield, strong regeneration, long service life, strong palatability, and rich nutrition. So far, alfalfa has been planted in my country for more than two thousand years, and it is also the pasture with the largest planting area in my country. Yet a kind of worldwide disease of alfalfa, root rot (Rootrot), can harm each growth stage of alfalfa, and main symptom is that necrosis spot appears in root, even rots, dies, and stem and leaf are all withered, have a strong impact on the growth of alfalfa. Quality and yield, this disease will also increase with the growth of planting area and years. Although the pathogen of alfa...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/125
Inventor 郭长虹程园园刘冠一张丹刘莎莎刘博文
Owner HARBIN NORMAL UNIVERSITY
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