Method for measuring effective content of acanthopanax in medicine

Abstract

The invention relates to a method for measuring the effective content of acanthopanax root in medicine. Based on the chromatographic conditions of high-performance liquid chromatography measurement, the method uses octadecyl bonded silica as the filler, and takes methanol-water as the mobile phase, with the detection wavelength reaching 265nm; the theoretical plate number is not less than 3000 in accordance with syringin wave peak calculation; firstly, the reference substance solution with 0.02mg of syringin in each 1ml of the solution is prepared; then 1g of the measured medicine is put into a 50ml-measuring flask and diluted to 50ml scale with methanol; 5ml of subsequent filtrate is processed through decompression and drying and then added to a treated C18 solid extraction column; 4ml of 30% methanol eluate and 30% methanol solution are collected and diluted to 5ml scale so as to prepare the sample solution; 10ul of the reference substance solution and 10ul of the sample solution are respectively extracted and injected into the liquid chromatography to measure the acanthopanax root content based on syringin. The method is convenient and fast and has accurate measurement standards to ensure the product quality and efficacy of the medicine containing acanthopanax root.

Description

technical field [0001] The invention relates to a method for measuring the content of a single component of a Chinese patent medicine, in particular to a method for measuring the effective content of Acanthopanax senticosus in the medicine. Background technique [0002] Modern studies have shown that the traditional Chinese medicine Acanthopanax is beneficial to Qi and spleen, tonifies the kidney and calms the nerves, dispels rheumatism, strengthens bones and muscles, promotes blood circulation and removes blood stasis, nourishes the middle and nourishes essence, strengthens intelligence, strengthens stomach and diuresis, etc. Acanthopanax contains syringin (C 17 h 24 o 9 ), anti-aging, anti-fatigue, anti-cancer, and regulating nerve, endocrine, cardiovascular function, improving the body's resistance to physical, chemical, biological and other harmful stimuli and other pharmacological effects. Neurasthenia, insomnia, hypertension, coronary heart disease, cerebral infarct...

AI Technical Summary

Problems solved by technology

Due to the different preparation methods, the active ingredients (content) of Acanthopanax are extracted differently, which results in the lack of necessary determination methods for the analysis and inspection of the curative effect of the medicine, resulting in the lack of standards and basis for the content of the active ingredients of Acanthopanax in terms of quality control.