Method for measuring effective content of acanthopanax in medicine

A technology of effective content and Acanthopanax senticosus, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of lack of standards and basis for the content of Acanthopanax senticosus active ingredients, lack of measurement methods for drug efficacy analysis and testing, and achieve The detection method is convenient and quick, the determination standard is accurate, and the effect of ensuring product quality and drug efficacy

Inactive Publication Date: 2009-02-18
山东仙河药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the different preparation methods, the active ingredients (content) of Acanthopanax are extracted differently, which results in the lack of necessary determination methods for the anal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Embodiment 1: Utilize high-performance liquid chromatography to measure the content of syringin in the extract of Acanthopanax senticosus.

[0009] For chromatographic conditions, octadecyl bonded silica gel is used as filler; methanol-water (22:78) is used as mobile phase, the detection wavelength is 265nm, and the number of theoretical plates should not be less than 3000 based on the calculation of syringin peak.

[0010] Accurately weigh 2mg of syringin reference substance, put it in a 100ml measuring bottle, dissolve it with 50% methanol, dilute to the mark, and obtain a reference substance solution containing 0.02mg of syringin in every 1ml. Take the contents under the volume difference of Acanthopanax acanthopanax extract, mix well, grind finely, take 1g and put it in a 50ml measuring bottle, add 40ml of methanol, ultrasonicate for 30 minutes, and dilute to the mark with methanol. Filter, accurately measure 5ml of the subsequent filtrate and evaporate to dryness u...

Embodiment 2

[0011] Example 2: Using high performance liquid chromatography to measure the syringin content of Acanthopanax in Acanthopanax capsules.

[0012] For chromatographic conditions, octadecyl bonded silica gel is used as filler; methanol-water (22:78) is used as mobile phase, the detection wavelength is 265nm, and the number of theoretical plates should not be less than 3000 based on the calculation of syringin peak.

[0013] Accurately weigh 2 mg of the syringin reference substance, put it in a 100ml measuring bottle, dissolve it with 50% methanol, dilute to the mark, and shake well to obtain a reference substance solution containing 0.02 mg of syringin in every 1 ml. Take the content of Acanthopanax Capsules under the item of filling difference, mix and grind finely, take 1g and put it in a 50ml measuring bottle, add 40ml of methanol, sonicate for 30 minutes, and dilute to the mark with methanol. After filtration, take 5ml of the filtrate and evaporate to dryness under reduced p...

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Abstract

The invention relates to a method for measuring the effective content of acanthopanax root in medicine. Based on the chromatographic conditions of high-performance liquid chromatography measurement, the method uses octadecyl bonded silica as the filler, and takes methanol-water as the mobile phase, with the detection wavelength reaching 265nm; the theoretical plate number is not less than 3000 in accordance with syringin wave peak calculation; firstly, the reference substance solution with 0.02mg of syringin in each 1ml of the solution is prepared; then 1g of the measured medicine is put into a 50ml-measuring flask and diluted to 50ml scale with methanol; 5ml of subsequent filtrate is processed through decompression and drying and then added to a treated C18 solid extraction column; 4ml of 30% methanol eluate and 30% methanol solution are collected and diluted to 5ml scale so as to prepare the sample solution; 10ul of the reference substance solution and 10ul of the sample solution are respectively extracted and injected into the liquid chromatography to measure the acanthopanax root content based on syringin. The method is convenient and fast and has accurate measurement standards to ensure the product quality and efficacy of the medicine containing acanthopanax root.

Description

technical field [0001] The invention relates to a method for measuring the content of a single component of a Chinese patent medicine, in particular to a method for measuring the effective content of Acanthopanax senticosus in the medicine. Background technique [0002] Modern studies have shown that the traditional Chinese medicine Acanthopanax is beneficial to Qi and spleen, tonifies the kidney and calms the nerves, dispels rheumatism, strengthens bones and muscles, promotes blood circulation and removes blood stasis, nourishes the middle and nourishes essence, strengthens intelligence, strengthens stomach and diuresis, etc. Acanthopanax contains syringin (C 17 h 24 o 9 ), anti-aging, anti-fatigue, anti-cancer, and regulating nerve, endocrine, cardiovascular function, improving the body's resistance to physical, chemical, biological and other harmful stimuli and other pharmacological effects. Neurasthenia, insomnia, hypertension, coronary heart disease, cerebral infarct...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 李保国耿鲁骏
Owner 山东仙河药业有限公司
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