Method for preparing snowdrop syringin

A technology of syringin and snow lotus is applied in the field of plant cell engineering to achieve the effects of high yield, protection of wild resources and improvement of yield

Inactive Publication Date: 2008-07-23
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, there is no report on cultivating high-yielding syringin with Saussurea sativa cells

Method used

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  • Method for preparing snowdrop syringin
  • Method for preparing snowdrop syringin
  • Method for preparing snowdrop syringin

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Embodiment Construction

[0030] In order to better understand the present invention, the following examples are used to further illustrate, but not limit the present invention.

[0031] In this embodiment, the preferred jellyfish Saussurea medusa is taken as an example to illustrate the present invention, and at the same time, the technical solution of this embodiment is also applicable to the cell culture of other Saussurea medusa.

[0032] Callus induction and cell line establishment of Saussurea sauvignon:

[0033] Collect snow lotus explants, such as leaves, flowers, stems, or germinate seedlings with seeds to induce callus. The explants were first rinsed with tap water for a few hours, then soaked in a bactericide for sterilization, washed 5-6 times with sterile water for 2-3 minutes each time, and inoculated on the hormone-containing MS medium shown in Table 1 after disinfection , placed at 25 (± 2) ℃ light culture. After two weeks of culture, callus tissue can be formed at the incision site o...

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Abstract

This invention relates to a cultivation method for cells of Xuelian lilac aglycons including: applying Xuelian explant or seeds to bourgeon young plants to induce calli on the 1 / 2 cultivation medium, said callus cells are used to produce Xuelian lilac aglycons, MS cultivation medium is the solid for generating the Xuelian lilac aglycon cells or for suspending cultivation, 70% alcohol or n-butyl alcohol is used in separating, extracting and preparing the aglycons and HPLC is used for testing its content. This invented content of gross lilac compound is 3-4% of the dry weight of cells in this invented cell cultivating material and its productivity is 400-900mg / L.

Description

technical field [0001] The invention belongs to the field of plant cell engineering, and in particular relates to a preparation method of Saussurea syringin. Background technique [0002] Snow lotus, also known as snow lotus, is one of the three rare plants in my country. As early as in "Northwest Territories" and "Xiangyuan Xiaozhi", there are records about snow lotus, and the two are combined into one, collectively called snow lotus. As snow lotus, the name of the original plant varies with the place of origin. For example, in Tibet and other places, S.tridactyla Sch.Bip is used. In Sichuan, Yunnan and other places, S.lanicps Hand.-Mazz. Gansu, Qinghai and other places are used. Snow lotus S.medusa Maxim; Qinghai also uses snow lotus S.gossypiphora D.Don; Xinjiang uses Tianshan snow lotus (Snow lotus) S.involucrata Kar.et Kir, etc., but they all belong to the genus Saussurea of ​​the family Asteraceae The subgenus Subgen Eriocorye plants are generally distributed on the a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 赵德修虞珍珍付春祥
Owner INST OF BOTANY CHINESE ACAD OF SCI
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