Method for extracting syringin compound from tobaccos

A technology of syringin and compounds, applied in the field of tobacco chemistry, can solve the problem of lack of antioxidant activity

Inactive Publication Date: 2017-04-26
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At the same time, the applicant found that the existing syringin does not have antioxidant activity, especially the method of extracting syringin from tobacco, which has not been reported so far, thus limiting its scope of application, especially syringin as a Addition of flavors and fragrances

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  • Method for extracting syringin compound from tobaccos
  • Method for extracting syringin compound from tobaccos
  • Method for extracting syringin compound from tobaccos

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Embodiment Construction

[0027] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0028] A syringin compound with antioxidant activity, the compound has the following structural formula:

[0029]

[0030] white crystals (CD 3 OD), Chemical Formula: C 17 h 24 o 9 , Exact Mass: 372.14, Molecular Weight: 372.37 m / z: 372.14 (100.0%), 373.15 (19.0%), 374.15 (3.6%), Elemental Analysis: C, 54.83; H, 6.50; O, 38.67.

[0031]

[0032]

[0033] A method for extracting syringin compounds from tobacco, comprising the following steps:

[0034] (1) After extracting the tobacco sample with 75% ethanol aqueous solution, obtain the tobacco extract by concentrating under reduced pressure;

[0035] (2) After the tobacco extract is dissolved in water and ultrasonically assisted, it is extracted with ethyl acetate or petroleum ether, and the extract i...

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Abstract

The invention discloses a method for extracting a syringin compound from tobaccos. The method is characterized by comprising the following steps: preparing a tobacco extract; dissolving the tobacco extract into water, and extracting; eluting an extraction solution with ethanol in a macroporous resin column; collecting eluate, performing reduced pressure concentration, performing capillary sampling thin-layer chromatography, and combining eluate containing the similar components; performing reduced pressure concentration till a solid state to obtain a polar tobacco extract; performing thin-layer chromatography, combining samples with amaranth spots, and performing reduced pressure concentration to obtain a crude extract; uniformly stirring the crude extract and silica gel, separating by using a normal phase silica gel chromatographic column, and eluting; through the chromatographic column, combining fragrance-forming substances according to different Rf values and different polarities to obtain a fine extract; performing normal phase column chromatography on the fine extract, and eluting, segmenting into 5 parts A-E during TLC detection, performing gel column chromatography on a segment B, feeding the segment B into a semi-preparative chromatography system, and separating; under chromatographic conditions, retaining for 14.32 minutes, and separating to obtain the monomer compound.

Description

technical field [0001] The invention relates to a method for extracting syringin compounds from tobacco, which belongs to the technical field of tobacco chemistry. Background technique [0002] Syringin is a phenylpropanoid glycoside compound. Syringin monomer is often used as a quality control indicator for inspecting Chinese herbal medicines such as Acanthopanax, Zushima and Jiubingying. However, there are few reports about the extraction and isolation of syringin from plants. Many plants in nature contain syringin, but because of its low syringin content, the preparation cost of syringin standard is relatively high. Therefore, the simple and efficient extraction and preparation of syringin is of great significance for the quality control of Chinese medicinal materials and the development of new drugs with syringin as the precursor. [0003] Syringin is a strong anti-hepatotoxic drug. It can restore the enzyme activity of the microsomal enzyme system and inhibit lipid p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08A24B15/26
CPCC07H15/203A24B15/26C07H1/08
Inventor 葛永辉耿召良王承福丁根胜李永青夏范讲向章敏张婕
Owner GUIZHOU TOBACCO SCI RES INST
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