Method for extracting scopoletin compounds from tobacco

A technology for scopolide and compound is applied in the field of extracting scopolide compound, and can solve problems such as the lack of mature and perfect industrial extraction methods and the like

Inactive Publication Date: 2017-05-31
GUIZHOU TOBACCO SCI RES INST
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported that it can be extracted from plants such as angelica and belladonna, there is no mature and perf

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting scopoletin compounds from tobacco
  • Method for extracting scopoletin compounds from tobacco
  • Method for extracting scopoletin compounds from tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0027] A scopoletin compound with antioxidant activity, the compound has the following structural formula:

[0028]

[0029] white powder (CD 3 OD), Chemical Formula: C10H8O4 Exact Mass: 192.04 Molecular Weight: 192.17 m / z: 192.04 (100.0%), 193.05 (11.1%), 194.05 (1.4%) Elemental Analysis: C, 62.50; H, 4.20; O, 33.30.

[0030]

[0031] A method for extracting scopoletin compounds from tobacco, the method adopts the following steps:

[0032] (1) After extracting the tobacco sample with 75% ethanol aqueous solution, obtain the tobacco extract by concentrating under reduced pressure;

[0033] (2) After the tobacco extract is dissolved in water and ultrasonically assisted, it is extracted with ethyl acetate or petroleum ether, and the extract is concentrated u...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for extracting scopoletin compounds from tobacco. The method is characterized by comprising the following steps of preparing tobacco extracts; performing water dissolution on the tobacco extracts; then, performing extraction; eluting the extraction liquid in a macroporous resin chromatographic column by ethyl alcohol; after eluant is collected, performing reduced pressure concentration; then, performing capillary tube sampling thin-layer chromatography; merging the eluant with the similar ingredients; performing reduced pressure concentration until the materials are dry so that a tobacco polar extract is obtained; merging samples showing brownish black spots by a thin-layer chromatography method; performing reduced pressure concentration; obtaining coarse extracts; uniformly stirring the coarse extracts and silica gel; using a positive phase silica gel column for separation and elution; by using the thin-layer chromatography method, merging flavor matters according to different Rf values in accordance with different polarities to obtain fine extracts; performing positive phase column chromatography on the fine extracts; performing elution; segmenting TLC detection into five parts from A to E; performing reverse phase silica gel column chromatography on the section B; then, feeding the sample to a half preparation chromatography system for separation; under each chromatography condition, maintaining the time for 7.23 minutes; and performing separation to obtain the monomeric compound.

Description

technical field [0001] The invention relates to a method for extracting scopoletin compounds from tobacco, belonging to the technical field of tobacco chemistry. Background technique [0002] Scopoletin, also known as scopoletin, belongs to coumarin compounds, often used as drugs for analgesia, anti-inflammation, lowering blood pressure and relieving muscle spasm. It has been reported that it can be extracted from various plants, such as Angelica, Belladonna, etc. Scopoletin has antibacterial activity against different types of bacteria, including Escherichia coli, Staphylococcus aureus, Streptococcus, Klebsiella pneumoniae and Pseudomonas aeruginosa. Scopoletin has anti-inflammatory activity and is useful in the treatment of bronchial diseases and asthma. It also regulates the hormone serotonin, which helps reduce anxiety and depression. [0003] However, the synthesis process of scopoletin is relatively complicated and the cost is high. It can only be obtained from pla...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D311/16A61P39/06A24B15/40
CPCA24B15/403C07D311/16
Inventor 耿召良葛永辉王承福丁根胜李永青夏范讲向章敏张婕
Owner GUIZHOU TOBACCO SCI RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap