Method for extracting scopoletin compounds from tobacco

Abstract

The invention discloses a method for extracting scopoletin compounds from tobacco. The method is characterized by comprising the following steps of preparing tobacco extracts; performing water dissolution on the tobacco extracts; then, performing extraction; eluting the extraction liquid in a macroporous resin chromatographic column by ethyl alcohol; after eluant is collected, performing reduced pressure concentration; then, performing capillary tube sampling thin-layer chromatography; merging the eluant with the similar ingredients; performing reduced pressure concentration until the materials are dry so that a tobacco polar extract is obtained; merging samples showing brownish black spots by a thin-layer chromatography method; performing reduced pressure concentration; obtaining coarse extracts; uniformly stirring the coarse extracts and silica gel; using a positive phase silica gel column for separation and elution; by using the thin-layer chromatography method, merging flavor matters according to different Rf values in accordance with different polarities to obtain fine extracts; performing positive phase column chromatography on the fine extracts; performing elution; segmenting TLC detection into five parts from A to E; performing reverse phase silica gel column chromatography on the section B; then, feeding the sample to a half preparation chromatography system for separation; under each chromatography condition, maintaining the time for 7.23 minutes; and performing separation to obtain the monomeric compound.

Description

technical field [0001] The invention relates to a method for extracting scopoletin compounds from tobacco, belonging to the technical field of tobacco chemistry. Background technique [0002] Scopoletin, also known as scopoletin, belongs to coumarin compounds, often used as drugs for analgesia, anti-inflammation, lowering blood pressure and relieving muscle spasm. It has been reported that it can be extracted from various plants, such as Angelica, Belladonna, etc. Scopoletin has antibacterial activity against different types of bacteria, including Escherichia coli, Staphylococcus aureus, Streptococcus, Klebsiella pneumoniae and Pseudomonas aeruginosa. Scopoletin has anti-inflammatory activity and is useful in the treatment of bronchial diseases and asthma. It also regulates the hormone serotonin, which helps reduce anxiety and depression. [0003] However, the synthesis process of scopoletin is relatively complicated and the cost is high. It can only be obtained from pla...

AI Technical Summary

Problems solved by technology

Although it has been reported that it can be extracted from plants such as angelica and belladonna, there is no mature and perfect industrial extraction method, especially the method of extracting scopoletin from tobacco, which has not been reported yet.

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