The invention relates to the technical field of edible mushrooms, in particular to a lepista nuda new strain and a cultivation method and application thereof, and the lepista nuda ZDX-1 is obtained by collecting wild resources in Qingcheng County in Qingyang City of Gansu Province, domesticating and cultivating the wild resources and then systematically screening the wild resources. The
diameter of
pileus is 3-6 cm, the
pileus is hemispherical in the early stage, gradually flattened after maturing, smooth in surface, free of flakes, dark purple brown, uniform in color and luster, and not prone to
fading. The pleats grow straightly to be bent and are densely arranged, and the color is gradually changed into taupe from light purple. The stipe is cylindrical, upright or slightly bent, the length is 6-10cm, the thickness is 0.8-2cm, the base part is slightly expanded, the color of the stipe is similar to that of a
pileus, and the stipe is
solid inside and compact in meat quality. According to hyphae of the ZDX-1, bacterial colonies are round, the edges are neat, aerial hyphae are developed, thick and strong, white to light purple and extend towards the periphery in a radial mode, and the hyphae grow compactly. The growth vigor of the culture medium taking glucose as a
carbon source and the culture medium taking peptone as a
nitrogen source is good on the culture medium with the pH value of 7; the hyphae are observed under microscopic conditions, and the hyphae are relatively dense, have a lock-like joint phenomenon, are not isolated, have coiled conidiophores, can generate a large number of spores, and can be applied to various fields of
food preparation.