Extraction and separation method of syringin

The technology of a syringin and a separation method, which is applied in the field of chemical purification and preparation of natural medicines, can solve the problems of high preparation cost and low content of syringin, and achieves low cost, convenient industrial production, good stability and reproducibility. Effect

Inactive Publication Date: 2008-12-24
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports about extracting and isolating syringin from plants.
Many plants in nature contain syringin, but because of its low syringin content, the preparation cost of syringin standard is relatively high

Method used

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  • Extraction and separation method of syringin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Extraction of syringin

[0017] Take 0.5Kg of white clove (Syringa oblata Lindl.var.alba Hort.ex Rehd.) branch and grind it, soak it in water for 2h, wherein the ratio of material to liquid is 1:20 (g / ml), decoct twice, each decoction 1h, filtered and concentrated to obtain syringin extract;

[0018] 2) Separation of syringin

[0019] Adsorb the syringin extract with an AB-8 macroporous adsorption resin column at a flow rate of 1BV / h, then wash the macroporous adsorption resin column after the adsorbed sample with water, and then elute with an ethanol solution with a volume concentration of 30%. Silicone GF 254 Rapid qualitative detection with CMC-Na thin-layer plate, wherein the developer is chloroform:methanol 8:2, the chromogenic agent is sulfuric acid ethanol solution with a volume concentration of 10%, and the syringin eluate is collected;

[0020] Concentrate the eluate into an extract, dissolve it with 25ml of methanol and mix it with 70g of silica gel to m...

Embodiment 2

[0024] 1) Extraction of syringin

[0025] Take 1Kg of white clove (Syringa oblata Lindl.var.alba Hort.ex Rehd.) branches and crush them, soak them in water for 3 hours, the ratio of solid to liquid is 1:30 (g / ml), decoct 3 times, each time for 3 hours , filtered, and concentrated to obtain syringin extract;

[0026] 2) Separation of syringin

[0027] Adsorb the syringin extract with an XDA-1 macroporous adsorption resin column at a flow rate of 1BV / h, then wash the macroporous adsorption resin column after the adsorbed sample with water, and then elute with an ethanol solution with a volume concentration of 20%. Silicone GF 254 Rapid qualitative detection with CMC-Na thin-layer plate, wherein the developer is chloroform:methanol 8:2, the chromogenic agent is sulfuric acid ethanol solution with a volume concentration of 10%, and the syringin eluate is collected;

[0028] Concentrate the eluent into an extract, dissolve it with 35ml of methanol and mix it with 100g of silica ...

Embodiment 3

[0032] 1) Extraction of syringin

[0033] Take 0.75Kg of white clove (Syringa oblata Lindl.var.alba Hort.exRehd.) branch and grind it, soak it in water for 1h, and the ratio of solid to liquid is 1:25 (g / ml), decoct 3 times, each time for 2h , filtered, and concentrated to obtain syringin extract;

[0034] 2) Separation of syringin

[0035] Adsorb the syringin extract with an AB-8 macroporous adsorption resin column at a flow rate of 2BV / h, then wash the macroporous adsorption resin column after the adsorbed sample with water, and then elute with an ethanol solution with a volume concentration of 60%. Silicone GF 254 Rapid qualitative detection with CMC-Na thin-layer plate, wherein the developer is chloroform:methanol 8:2, the color developer is sulfuric acid ethanol solution with a volume concentration of 10%, and the syringin eluate is collected;

[0036] Concentrate the eluent into an extract, dissolve it with 30ml of methanol and mix it with 90g of silica gel to make a ...

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Abstract

The invention relates to a method for extracting and separating ligustrin, which is to extract the ligustrin by the decoction method through crushing of excrementum passeris (Syringa oblata Lindl.var. alba Hort.ex Rehd.) branches. A ligustrin extract is eluted by 20 to 60 percent alcohol solution through an XDA-1 or AB-8 macroporous absorption resin column, and undergoes chromatographic separation through a silica gel column, and then ligustrin crystals with the purity over 97 percent can be obtained. The method comprises the following techniques: extraction of the ligustrin from the excrementum passeris, separation of macroporous resins and the silica gel column, crystallization and so on. The method has the advantages of low cost, simple and convenient operation, convenient industrialized production and so on. Both the stability and the reproducibility of the techniques are good; the purity of products obtained is more than 97 percent; and the method is suitable for extraction in mass production.

Description

technical field [0001] The invention belongs to the field of chemical purification and preparation of natural medicines, in particular to a method for extracting and separating syringin. Background technique [0002] Syringin is a phenylpropanoid glycoside compound. Syringin monomer is often used as a quality control indicator for inspecting Chinese herbal medicines such as Acanthopanax, Zushima and Jiubingying. However, there are few reports about the extraction and isolation of syringin from plants. There are many plants containing syringin in nature, but because of the low content of syringin, the preparation cost of syringin standard is relatively high. Therefore, the simple and efficient extraction and preparation of syringin is of great significance for the quality control of traditional Chinese medicinal materials and the development of new drugs using syringin as a precursor. Contents of the invention [0003] The purpose of the present invention is to provide a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08
Inventor 齐香君郑艳李楠杨文娟邵鹏武攀樊国彪
Owner SHAANXI UNIV OF SCI & TECH
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