Method for promoting growth of natural calculus bovis by using novel small molecule peptide combination

Abstract

The invention belongs to the technical field of biological agriculture, and particularly relates to a method for promoting growth of natural calculus bovis by using a novel small molecule peptide combination, which comprises the following steps: selecting a plurality of small molecule peptides with specific functions, and mixing the small molecule peptides according to a specific proportion to form a small molecule peptide combination with a synergistic effect. The growth of the natural calculus bovis can be effectively promoted, and the yield and the quality of the natural calculus bovis are improved. Meanwhile, the method disclosed by the invention has relatively high safety and has a wide prospect in practical application. Animal experiment data further verifies the effectiveness and safety of the method.

Description

Technical Field

[0001] The invention relates to the technical field of natural bezoar production, and in particular to a method for promoting the growth of natural bezoar by utilizing a novel small molecule peptide combination. Background Art

[0002] As a precious Chinese medicinal material, natural bezoar has extremely high medicinal value and market demand. However, due to the complex formation process and limited output of natural bezoar, it is difficult to meet the market demand. At present, people have been exploring various methods to promote the growth of natural bezoar to increase its output.

[0003] As a small molecule compound with biological activity, small molecule peptides have the characteristics of small molecular weight, easy absorption, and high biological activity. In recent years, small molecule peptides have been widely used in the fields of medicine, food, cosmetics, etc. Studies have shown that some small molecule peptides have the effects of promoting bile secretion, regulating liver metabolism, and enhancing cell vitality, which may help promote the growth of natural bezoar.

[0004] However, the existing method of promoting the growth of natural bezoar using small molecule peptides has some shortcomings, such as the single type of small molecule peptides, limited effect, poor stability, etc. Therefore, it is necessary to develop a new method of promoting the growth of natural bezoar using a combination of multiple small molecule peptides to improve the effect and stability of small molecule peptides and enhance their ability to promote the growth of natural bezoar. Summary of the invention

[0005] The method of promoting the growth of natural bezoar by using a novel small molecule peptide combination of the present invention mainly comprises the steps of preparing a small molecule peptide mixed solution, selecting a suitable animal donor, administration method, monitoring and management, and collection of natural bezoar. By administering a specific small molecule peptide mixed solution to the donor cattle, the liver metabolism can be regulated, bile secretion can be promoted, and cell activity can be enhanced, thereby creating favorable conditions for the growth of natural bezoar.

[0006] The present invention provides a method for promoting the growth of natural bezoar by using a novel small molecule peptide combination, comprising the following steps: Step 1: Preparation of small molecule peptide mixed solution A variety of specific small molecule peptides are selected, and the small molecule peptides include: Small molecule peptide E: It has the function of regulating liver cell metabolic pathways and enhancing the activity of key enzymes in bile synthesis; small molecule peptide F: It can stimulate gallbladder contraction, promote bile discharge, and provide a dynamic environment for the growth of bezoar; small molecule peptide G: It helps to maintain the appropriate pH and ion concentration in bile, which is conducive to the formation of bezoar precipitation; these small molecule peptides are mixed in a specific ratio; Step 2: Select the appropriate animal donor Select healthy cattle that meet specific standards as donor animals for natural bezoar growth; Step 3: Dosage administering the small molecule peptide mixed solution to the donor cattle through a specific administration route, which may be oral administration or intragastric administration; Step 4: Monitoring and Management After administration of the small molecule peptide mixture solution, the donor cattle were regularly monitored, including observation of their physiological state, appetite, feces, etc., as well as blood tests and liver ultrasound examinations; Step 5: Collection of natural bezoar After a certain period of growth, when it is determined through monitoring that the natural bezoar has reached a mature state that can be collected, appropriate methods are used to collect the natural bezoar.

[0007] Furthermore, the method of promoting the growth of natural bezoar using a new small molecule peptide combination, the small molecule peptides include: small molecule peptide E: amino acid sequence is SEQNO1: Glu - Ala - Trp - Lys - Phe - Thr -Gly - Val, molecular weight range is 700 - 1100 Daltons; small molecule peptide F: amino acid sequence is SEQNO2: Asp -Ser - Leu - Pro - Ile - Tyr - Gln - Lys, molecular weight is 800 - 1200 Daltons; small molecule peptide G: amino acid sequence is SEQNO3: Met - Cys - Val - His - Arg - Asp - Leu - Thr, molecular weight is 850 - 1250 Daltons.

[0008] Furthermore, in the method of promoting the growth of natural bezoar using a new small molecule peptide combination, the small molecule peptides are mixed in a specific ratio, the mixing ratio is small molecule peptide E: small molecule peptide F: small molecule peptide G = 2:1:1; the small molecule peptide combination is dissolved in an appropriate solvent to prepare a small molecule peptide mixed solution with a concentration of 15 mg / ml, and the solvent is physiological saline.

[0009] Furthermore, in the method of promoting the growth of natural bezoar using a novel small molecule peptide combination, the cattle should have good physique and liver function, be 2-3 years old, and weigh 400-500kg.

[0010] Furthermore, in the method of promoting the growth of natural bezoar using a novel small molecule peptide combination, after administering the small molecule peptide mixed solution, the donor cow is regularly monitored, including observing its physiological state, appetite, and feces, as well as performing blood tests and liver ultrasound examinations to evaluate the effects of the small molecule peptide combination on the donor cow and the growth of natural bezoar; based on the monitoring results, the dosage and frequency of the small molecule peptide combination are adjusted.

[0011] Beneficial technical effects of the invention: Combination of multiple small molecule peptides: A combination of three small molecule peptides with different functions is used to synergistically promote the growth of natural bezoar, thereby improving the effect of promoting the growth of natural bezoar.

[0012] Specific mixing ratio: The optimal small molecule peptide mixing ratio is determined through experiments to optimize the synergistic effect of the small molecule peptide combination, further improving the effect of promoting the growth of natural bezoar.

[0013] Multiple administration methods: can be selected according to the specific conditions and actual needs of the donor cows, which improves the absorption effect and bioavailability of small molecule peptides.

[0014] Advanced monitoring and management: Using advanced detection technology and equipment, we regularly monitor and manage the donor cattle, and timely adjust the dosage and frequency of administration, as well as feeding and management measures, to ensure that the growth of natural bezoar is carried out under safe and effective conditions.

[0015] Large-scale application: This method can be applied to large-scale natural bezoar production. By optimizing production processes and management measures, the output and quality of natural bezoar can be improved to meet market demand. DETAILED DESCRIPTION Embodiment 1:

[0016] (1) Preparation of small molecule peptide mixed solution Selection of small molecule peptides: Specific small molecule peptides that promote the growth of natural bezoar are selected, including small molecule peptide E, small molecule peptide F and small molecule peptide G. The amino acid sequences of these small molecule peptides are different, and they have different biological functions, which can synergistically promote the growth of natural bezoar. Small molecule peptide E: The amino acid sequence is SEQNO1: Glu-Ala-Trp-Lys-Phe-Thr-Gly-Val, and the molecular weight range is 700-1100 Daltons. It has the function of regulating the metabolic pathway of liver cells and enhancing the activity of key enzymes in bile synthesis. This small molecule peptide can act on liver cells, regulate the metabolic pathway of liver cells, promote the expression and activity of key enzymes in bile synthesis, thereby increasing the amount of bile synthesis and providing a material basis for the growth of natural bezoar. Small molecule peptide F: The amino acid sequence is SEQNO2: Asp-Ser-Leu-Pro-Ile-Tyr-Gln-Lys, and the molecular weight is 800-1200 Daltons. It can stimulate gallbladder contraction, promote bile discharge, and provide a dynamic environment for the growth of bezoar. This small molecule peptide can act on gallbladder cells, stimulate gallbladder contraction, promote bile discharge, make bile flow more smoothly in the gallbladder, and provide a dynamic environment for the growth of natural bezoar. Small molecule peptide G: The amino acid sequence is SEQNO3: Met - Cys - Val - His - Arg - Asp - Leu - Thr, with a molecular weight of 850 - 1250 Daltons, which helps maintain the appropriate pH and ion concentration in bile, and is conducive to the formation of bezoar precipitation. The small molecule peptides abbreviated as "EAWKFTGV", "DSLPIYQK, "MCVHRDLT" can regulate the pH and ion concentration of bile, making it more conducive to the precipitation of natural bezoar. These small molecule peptides are mixed in a specific ratio to form a synergistic small molecule peptide combination. The mixing ratio is small molecule peptide E: small molecule peptide F: small molecule peptide G = 2:1:1. The optimal mixing ratio is determined by experiments to achieve the best effect of promoting the growth of natural bezoar. The small molecule peptide combination is dissolved in an appropriate solvent to prepare a small molecule peptide mixed solution with a concentration of 15 mg / ml. The solvent is physiological saline, which should have good biocompatibility and stability and will not adversely affect the activity of the small molecule peptides.

[0017] (2) Selecting the right animal donor Selection of donor cattle: Select healthy cattle that meet specific standards as donor animals for the growth of natural bezoar. Donor cattle should have good physique and liver function, be 2-3 years old, and weigh 400-500kg. The preferred breed is Simmental cattle, which has a stable genetic background and strong adaptability to the environment, which is conducive to improving the growth quality and yield of natural bezoar.

[0018] Physical examination: A comprehensive physical examination is conducted on the donor cattle, including blood tests, liver ultrasound examinations, etc., to ensure that they have no potential diseases and infections to ensure the quality and safety of natural bezoar growth.

[0019] (3) Dosage method Oral gavage: The small molecule peptide mixed solution is given to the donor cattle by oral gavage. 50 ml is given to each cow twice a day. The oral gavage method is simple to operate and easy to implement, but there may be problems with incomplete absorption.

[0020] Subcutaneous injection: The small molecule peptide mixed solution is given to the donor cattle by subcutaneous injection. The injection dose is determined according to the weight of the donor cattle, which is 3 ml per 100 kg body weight, once every 3 days. Subcutaneous injection can ensure the accurate dosage and rapid absorption of small molecule peptides, but the operation is relatively complicated and requires certain techniques and equipment.

[0021] (4) Monitoring and management Physiological status observation: After administration of the small molecule peptide mixed solution, the donor cattle are regularly observed, including observation of their appetite, feces, mental state, etc., to evaluate the effect of the small molecule peptide combination on the donor cattle.

[0022] Blood test: Blood tests are performed regularly on donor cattle to test their liver function, bile acid levels, blood routine and other indicators to understand the effects of the small molecule peptide combination on liver metabolism and bile secretion of donor cattle.

[0023] Liver ultrasound examination: Through liver ultrasound examination, the shape, size, stone formation, etc. of the donor cow gallbladder are observed to evaluate the growth of natural bezoar.

[0024] Adjust the dosage and frequency of administration: According to the monitoring results, adjust the dosage and frequency of the small molecule peptide combination, as well as the feeding and management measures for the donor cattle to ensure that the growth of natural bezoar is carried out under safe and effective conditions.

[0025] Feeding environment management: Strictly manage the feeding environment of donor cattle to keep the cattle shed clean, ventilated, and at appropriate temperature and humidity.

[0026] (5) Collection of natural bezoar Maturity judgment: After a certain period of growth, when it is determined through monitoring that the natural bezoar has reached a mature state that can be collected, appropriate methods are used to collect the natural bezoar.

[0027] Collection method: The method of collecting natural bezoar should minimize the harm to the donor cow while ensuring the quality and integrity of natural bezoar. Common collection methods include surgical removal and gallbladder puncture.

[0028] Care and treatment: After collecting natural bezoar, the donor cow will receive necessary care and treatment to promote its recovery.

[0029] The small molecule peptide combination of the present invention promotes the growth of natural bezoar in the following aspects: Regulate liver metabolism: Small molecule peptide E can regulate the metabolic pathways of liver cells, enhance the activity of key enzymes in bile synthesis, and increase the amount of bile synthesis. Bile is an important material basis for the formation of natural bezoars. Increasing the amount of bile synthesis can provide more material sources for the growth of natural bezoars.

[0030] Promote bile discharge: Small molecule peptide F can stimulate gallbladder contraction and promote bile discharge. The discharge of bile can make the bile flow in the gallbladder smoother, providing a dynamic environment for the growth of natural bezoar. At the same time, the discharge of bile can also avoid bile stasis and reduce factors such as gallstones that are not conducive to the growth of natural bezoar.

[0031] Maintain a suitable bile environment: Small molecule peptide G can regulate the pH and ion concentration of bile, making it more conducive to the precipitation and formation of natural bezoar. A suitable bile environment can promote the precipitation and crystallization of natural bezoar and improve the yield and quality of natural bezoar.

[0032] 100 healthy Simmental cattle were selected as experimental subjects and randomly divided into an experimental group and a control group, with 50 cattle in each group. The experimental group adopted the method of promoting the growth of natural bezoar by using a novel small molecule peptide combination of the present invention, that is, administering a small molecule peptide mixed solution and performing corresponding monitoring and management. The control group adopted a traditional feeding method without administering a small molecule peptide mixed solution.

[0033] Growth index: After 8 months of growth, the growth indexes such as weight and body length of the cattle in the experimental group and the control group were measured. The results showed that there was no significant difference in growth indexes between the cattle in the experimental group and the control group, indicating that the method of the present invention would not have an adverse effect on the normal growth and development of cattle. The average weight of the cattle in the experimental group was 480kg, and the average weight of the cattle in the control group was 475kg; the average body length of the cattle in the experimental group was 2.5m, and the average body length of the cattle in the control group was 2.45m.

[0034] Blood indexes: Blood tests were performed regularly on cattle in the experimental and control groups to detect liver function, bile acid levels, blood routine tests and other indicators. The results showed that after the cattle in the experimental group were given the small molecule peptide mixed solution, the liver function indicators were normal and the bile acid levels increased. The average bile acid level of cattle in the experimental group was 50 μmol / L, and the average bile acid level of cattle in the control group was 30 μmol / L. This shows that the small molecule peptide combination can promote bile secretion and create favorable conditions for the growth of natural bezoar. At the same time, the blood routine indicators were also within the normal range, indicating that the method of the present invention has no adverse effects on the blood system of cattle.

[0035] Natural bezoar yield: After a certain period of growth, natural bezoar was collected and weighed. The results showed that the average natural bezoar yield of cattle in the experimental group was 45g, while the average natural bezoar yield of cattle in the control group was 25g. The natural bezoar yield of the experimental group was significantly higher than that of the control group, indicating that the method of the present invention can effectively promote the growth of natural bezoar.

[0036] Safety assessment: During the experiment, the cattle in the experimental and control groups were closely observed, and their physiological state, appetite, feces, etc. were recorded. At the same time, the quality of the collected natural bezoar was tested to detect whether it contained harmful substances. The results showed that the cattle in the experimental group did not show obvious adverse reactions after being given the small molecule peptide mixed solution, and their physiological state was good, their appetite was normal, and there was no abnormality in their feces. No harmful substances were detected in the collected natural bezoar, indicating that the method of the present invention has high safety.

[0037] The present invention provides a method for promoting the growth of natural bezoar by using a novel small molecule peptide combination. The method selects a plurality of small molecule peptides with specific functions and mixes them in a specific ratio to form a small molecule peptide combination with a synergistic effect, which can effectively promote the growth of natural bezoar and improve the yield and quality of natural bezoar. At the same time, the method of the present invention has high safety and has broad prospects in practical applications. Animal experimental data further verify the effectiveness and safety of the method of the present invention.

Claims

1. A method for promoting the growth of natural bezoar using a novel small molecule peptide combination, characterized in that: The following steps are involved: Step 1: Preparation of small molecule peptide mixed solution A variety of specific small molecule peptides are selected, and the small molecule peptides include: Small molecule peptide E: has the function of regulating liver cell metabolic pathways and enhancing the activity of key enzymes in bile synthesis; small molecule peptide F: stimulates gallbladder contraction, promotes bile discharge, and provides a dynamic environment for the growth of bezoar; small molecule peptide G: maintains the appropriate pH and ion concentration in bile, which is conducive to the formation of bezoar precipitation; the above small molecule peptides are mixed in proportion; Step 2: Select the appropriate animal donor Select healthy cattle as donor animals for natural bezoar growth; Step 3: Dosage The small molecule peptide mixed solution is administered to the donor cow via an administration route; Step 4: Monitoring and Management After administration of the small molecule peptide mixture solution, the donor cows were regularly monitored, with blood tests and liver ultrasound examinations; Step 5: Collection of natural bezoar When it is determined through monitoring that the natural bezoar has reached a mature state that can be collected, the natural bezoar is collected; The amino acid sequence of the small molecule peptide E is: SEQ NO 1: Glu - Ala - Trp - Lys - Phe - Thr -Gly - Val; the amino acid sequence of the small molecule peptide F is: SEQ NO 2: Asp - Ser - Leu - Pro - Ile - Tyr- Gln - Lys; the amino acid sequence of the small molecule peptide G is: SEQ NO 3: Met - Cys - Val - His - Arg -Asp - Leu - Thr; the mixed mass ratio of the small molecule peptides is: small molecule peptide E: small molecule peptide F: small molecule peptide G = 2:1:1; the small molecule peptide combination is dissolved in a solvent to prepare a small molecule peptide mixed solution with a concentration of 15 mg / ml, and the solvent is physiological saline.

2. The method for promoting the growth of natural bezoar by using a novel small molecule peptide combination according to claim 1, characterized in that: The cattle should have good physique and liver function, be 2-3 years old, and weigh 400-500kg.

3. The method for promoting the growth of natural bezoar by using a novel small molecule peptide combination according to claim 1, characterized in that: After administration of the small molecule peptide mixed solution, the donor cattle are regularly monitored, including observation of their physiological state, appetite, feces, as well as blood tests and liver ultrasound examinations to evaluate the effects of the small molecule peptide combination on the donor cattle and the growth of natural bezoar; based on the monitoring results, the dosage and frequency of the small molecule peptide combination are adjusted.