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Gellan gum oligosaccharide and application thereof in prebiotics

A gum oligosaccharide and gellan gum technology, applied in the biological field, can solve the problems of high cost, real-time monitoring of animal intestinal changes, and long animal experiment cycle

Pending Publication Date: 2021-12-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on gellan gum oligosaccharides as prebiotics
[0004] At present, most of the studies on the beneficial effects of fermentation of oligosaccharides focus on animal experiments. The animal experiment period is long, the cost is high, and the changes in the animal intestines cannot be monitored in real time during the experiment.

Method used

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  • Gellan gum oligosaccharide and application thereof in prebiotics
  • Gellan gum oligosaccharide and application thereof in prebiotics
  • Gellan gum oligosaccharide and application thereof in prebiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1: the preparation of gellan gum oligosaccharide

[0061] Specific steps are as follows:

[0062] (1) Add 10 g of gellan gum to 1 L of deionized water, stir continuously to mix; add HCl to the reaction system to make the final concentration of HCl reach 0.4M; stir and hydrolyze the hydrolysis system in a water bath at 80°C for 36 hours.

[0063] (2) Adjust the pH of the hydrolyzed system in step (1) to 7.0 with 5-10M NaOH, and pass the solution through a microporous membrane (0.45 μm in diameter). Concentrate the supernatant to 1 / 12 of the original volume by using a rotary evaporator to obtain a concentrated solution.

[0064] (3) Add 95% ethanol to the concentrated solution so that the final concentration of ethanol reaches 70-75%, and let the mixture stand for more than 6 hours; centrifuge the solution at 8000r / min for 20 minutes to discard the precipitate.

[0065] (4) Add absolute ethanol to the supernatant to make the final concentration of ethanol rea...

Embodiment 2

[0067] Example 2: Monosaccharide composition analysis and molecular weight analysis of novel gellan gum oligosaccharides

[0068] Specific steps are as follows:

[0069] (1) The gellan gum oligosaccharide prepared in Example 1 is subjected to monosaccharide composition analysis, and the results are as follows:

[0070] The results showed that the monosaccharide composition of the new gellan gum oligosaccharides was glucose, rhamnose and glucuronic acid, and the molar ratio was [4~6]:[1.2~1.9]:[2.4~3.6].

[0071] (2) The molecular weight of the new gellan gum oligosaccharides was analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and the results are as follows:

[0072] The results showed that gellan gum oligosaccharides contained peaks with m / z 401,547,563,709,925,1071 ([M+2Na]+) and 379,525,541,687 ([M+Na]+) (see figure 2 ), corresponding to gellan gum oligosaccharides with molecular weights of 357, 503, 665, 881, and 1...

Embodiment 3

[0074] Example 3: Application of novel gellan gum oligosaccharides as prebiotics

[0075] Specific steps are as follows:

[0076] 1. In vitro static fecal bacteria fermentation method of gellan gum oligosaccharides

[0077] (1) Fresh human feces were collected from three healthy donors (one male and two females, aged 35-50 years, BMI 24.60-29.24 kg / m 2 ), collected with a sterile fecal collection tube, then placed in an anaerobic gas generating bag and placed on ice. Healthy donors were not taking probiotics or prebiotics for at least two months prior to stool sample donation;

[0078] (2) The fresh feces of the three individuals were diluted with sterile 0.1M PBS buffer to obtain a 10% (w / v) fecal dilution, and filtered through four layers of gauze sponge under aseptic conditions to remove food residue, resulting in a fecal slurry;

[0079] (3) The feces slurries of three people were inoculated into the basal nutrient medium at a ratio of 10% (v / v) respectively as a blank...

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Abstract

The invention discloses a gellan gum oligosaccharide and application thereof in prebiotics and belongs to the technical field of biology. The invention provides the gellan gum oligosaccharide, and a basic structure of a main chain of the gellan gum oligosaccharide comprises a tetrasaccharide unit, wherein the tetrasaccharide unit comprises D-glucose, D-glucuronic acid and L-rhamnose, a molar ratio of the D-glucose to the D-glucuronic acid to the L-rhamnose is (4-6): (1.2-1.9): (2.4-3.6), and the polymerization degree range of the gellan gum oligosaccharide is 2-6. Through in-vitro static and dynamic coprophilous fungus fermentation methods, the novel gellan gum oligosaccharide is found to be capable of significantly increasing the yield of acetic acid, propionic acid, butyric acid and total SCFAs, improving the structure of fecal flora and significantly improving the relative abundance of butyric acid producing bacteria.

Description

technical field [0001] The invention relates to a gellan gum oligosaccharide and its application in prebiotics, belonging to the field of biotechnology. Background technique [0002] Gellan gum is a safe microbial polysaccharide approved by the US Food and Drug Administration (FDA). It is mass-produced by Pseudomonas elodea at low cost and widely used in food industry, agriculture, chemical industry and life science. Gellan gum is difficult to digest by bifidobacteria and lactobacilli, limiting their potential as prebiotics. Oligosaccharides derived from gellan gum have potential as commercialized safe functional oligosaccharides. [0003] Oligosaccharides are a class of indigestible carbohydrates, usually composed of fewer than 10 monosaccharides. Increasingly, oligosaccharides are used as prebiotics to benefit health by modulating the activity of gut microbiota, stimulating the growth of beneficial bacteria, and accumulating terminal metabolites short-chain fatty acids (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/125A23L33/21A61K31/702A61K31/715A61P1/00
CPCA23L33/125A23L33/21A61K31/702A61K31/715A61P1/00A23V2002/00A23V2200/32A23V2250/5054
Inventor 詹晓北徐静静朱莉蒋芸吴剑荣张洪涛李志涛
Owner JIANGNAN UNIV
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