Coprophilous fungus composition and preparation method and purpose thereof

A technology of composition and fecal bacteria, which is applied in the field of piglet breeding, can solve the problems of unfavorable large-scale promotion and poor effect of diarrhea prevention and control, and achieve the effects of enhancing feeding compliance, good rheological properties, and improving taste

Inactive Publication Date: 2017-03-22
CHONGQING ACAD OF ANIMAL SCI
View PDF5 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN105029031A discloses a method for reducing the incidence of diarrhea in weaned piglets by oral administration of fecal bacteria transplantation; studies have shown that the method is less effective in preventing and treating diarrhea in lactating piglets, and the method needs to be prepared and used immediately. , raw materials, reagents and other conditions are not conducive to large-scale promotion

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Coprophilous fungus composition and preparation method and purpose thereof
  • Coprophilous fungus composition and preparation method and purpose thereof
  • Coprophilous fungus composition and preparation method and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Screening method for donor pigs and feces

[0047] Adult pigs over 300 days old (regardless of sex), the candidate individuals have no clinical symptoms of diarrhea and other major pig diseases. No antibiotics were used within 30 days before stool collection, and clear immunization / medication records were available. Screening method of donor pigs: using serology, antibody detection and other means, referring to GB / T22914-2008 "Control and Monitoring of SPF Pig Pathogens" to exclude common / severe infectious pathogens in pigs.

[0048] Table 1. Screening method for donor pigs

[0049]

[0050] Screening of donor feces: Refer to GB29921-2013 "Pathogen Limits in Food" standard for Salmonella, Listeria monocytogenes, Escherichia coli O157:H7, Staphylococcus aureus, Vibrio parahaemolyticus 5 detection of pathogenic bacteria.

[0051] Abnormal toxicity test: According to the general rule 1141 of the Pharmacopoeia of the People's Republic of China, abnormal toxicity test ...

Embodiment 2

[0054] The preparation steps of fecal bacteria freeze-dried powder are:

[0055] Obtaining the fecal suspension:

[0056] Add 1 part of fresh feces of healthy adult pigs to 9 parts of sterile saline at 4°C, shake and mix with a homogenizer; Centrifuge for 5 minutes, carefully collect the supernatant; centrifuge the collected supernatant at 4600 rpm for 15 minutes at 4°C, and pour off the supernatant; the precipitate collected by centrifugation is in a 1:1 ratio, and again Add sterile physiological saline at 4°C, centrifuge at 4,600 rpm for 15 minutes at 4°C, and pour off the supernatant to obtain the final stool suspension.

[0057] Fecal bacteria concentration = (wet fecal weight / final fecal suspension volume) × 100% (unit: g / mL)

[0058] Addition of protective agent:

[0059] Add 10% sterilized glycerol (V / V) by volume to the final stool suspension, mix well and store at -80°C, add 10% sterilized skim milk equal to the volume of the final stool suspension , and then add ...

Embodiment 3

[0063] The stability test of the feces bacterium freeze-dried powder preparation of embodiment 2

[0064] After the freeze-dried powder of fecal bacteria was sealed at room temperature and stored in the dark for 0 month and 3 months, samples were taken to detect the contents of Bacillus, Lactobacillus, Lactobacillus, Bifidobacterium and Streptococcus thermophilus in the powder. Bacillus was detected by LB plate dilution coating method. Lactobacillus, Lactobacillus, Bifidobacterium and Streptococcus thermophilus were detected by the national standard GB4789.35-2010 method. 5 batches were randomly selected, and the average value of the test results was calculated. The results are shown in Table 2:

[0065] Table 2

[0066]

[0067] Conclusion: After the fecal bacteria freeze-dried powder prepared in Example 2 was stored at room temperature and protected from light for 3 months, the number of viable bacteria remained at 10 7 More than CFU / g, it shows that the present invent...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
weightaaaaaaaaaa
Login to view more

Abstract

The invention provides a coprophilous fungus composition. According to the invention, fresh night soil of adult health pig is taken as a manure source, a coprophilous fungus final suspension is prepared by three-time centrifugation under specific low-temperature condition, a high molecular weight compound protective agent skimmed milk powder and a low molecular weight compound protective agent (glycerin, lactose, galactose, cane sugar, glucose, maltodextrin, sorbitol, adonitol and trehalose) are added, the coprophilous fungus composition is produced, which contains abundant Bacillus spp, lactic acid bacteria, lactobacillus, bifidobacteria, and streptococcus thermophilus, live bacteria number is kept at 108 CFU/g and more, the coprophilous fungus composition is used for preventing and treating diarrhoea of pigling at a lactation period, the prevention and treatment effects are obvious, the coprophilous fungus composition has more obvious effect for preventing and treating diarrhoea of pigling with 0-14 days (21-28 days early-stage weaning method) and 0-21 days (35 days weaning method). The preparation method of the coprophilous fungus composition is simple, the product shelf-life is long, the defect that the current coprophilous fungus transplant requires real-time preparation in the prior art, and the coprophilous fungus composition is suitable for industrial production.

Description

technical field [0001] The invention relates to piglet breeding, in particular to a fecal bacteria composition for reducing diarrhea in lactating piglets, a preparation method and application thereof. Background technique [0002] The intestinal tract is the immune organ with the largest contact area between animals and the external environment. It is a complex internal environment composed of food residues and decomposition products, enzymes, immunoglobulins, bacteria (symbiotic and pathogenic bacteria) and metabolites, viruses, etc. When each The balance of ingredients is broken, and the body is in an unhealthy state. Studies have shown that whether it is virus, bacteria, protozoa or animal's own function, feed and feeding management, environmental stress, drug-induced diarrhea in pigs, the time of diarrhea responds to the imbalance of intestinal flora; such as porcine epidemic diarrhea, toxic Escherichia coli K88 infection and the occurrence of neonatal diarrhea syndrome...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/24A61K9/50A61K9/19A61K47/46A61K47/10A61K47/26A61K47/36A61P1/12A23K50/30A23K10/18A23K50/60A23K10/20A23K20/163A61K35/742A61K35/744A61K35/747A61K35/745
CPCA61K35/24A61K9/19A61K9/5036A61K35/742A61K35/744A61K35/745A61K35/747A61K47/10A61K47/26A61K47/36A61K47/46A61K2300/00
Inventor 孙静葛良鹏刘作华丁玉春杜蕾黄勇曹浩然吴梦
Owner CHONGQING ACAD OF ANIMAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap