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Coprophilous fungi positioning transplantation method

A fecal bacteria and site technology, which is applied in the field of fecal bacteria localization and transplantation, can solve the problems of high technical requirements, complicated operations, and no prospect of conventional therapy, and achieves the effect of improving transplantation efficiency.

Active Publication Date: 2018-12-07
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of these methods of fecal bacteria transplantation are high technical requirements for operation, complicated operation, inconvenient, unacceptable pain for patients, affecting the quality of daily life, and long administration time.
These methods of fecal microbiota transplantation are not accepted by many clinicians and patients, and do not have the prospect of becoming a routine therapy

Method used

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  • Coprophilous fungi positioning transplantation method
  • Coprophilous fungi positioning transplantation method

Examples

Experimental program
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Effect test

Embodiment 1

[0044] (1) Material collection: put fresh feces into special feces collection buckets;

[0045] (2) Bacterial solution dilution: add 100g of feces to 400ml of ice saline (refrigerated at 4°C) and soak for 1 minute, and stir evenly with a stirrer for about 1 minute;

[0046] (3) Filtration: Filter sequentially with a stainless steel screen, and the mesh number of the filter screen increases step by step by 2mm, 1mm, 0.5mm, and 0.25mm to remove fecal residues and large particles, and collect the fecal suspension;

[0047] (4) Centrifugal cleaning: 3 times

[0048] The first centrifugation: 5 minutes at 2000 rpm, remove the supernatant, leave the sediment (5ml), add normal saline to 45ml, shake well;

[0049] The second centrifugation: 3 minutes at 2000 rpm, remove the supernatant, leave the sediment, add normal saline to 45ml, shake well;

[0050] The third centrifugation: 2000 rpm for 3 minutes. Remove the supernatant, keep the sediment, add physiological saline to 20ml, and...

Embodiment 2

[0057] (1) Material collection: put fresh feces into special feces collection buckets;

[0058] (2) Bacterial solution dilution: add 100g of feces to 500ml of ice saline (refrigerated at 4°C) and soak for 1 minute, and stir evenly with a stirrer for about 1 minute;

[0059] (3) Filtration: Filter sequentially with a stainless steel screen, and the mesh number of the filter screen increases step by step by 2mm, 1mm, 0.5mm, and 0.25mm to remove fecal residues and large particles, and collect the fecal suspension;

[0060] (4) Centrifugal cleaning: 2 times

[0061] The first centrifugation: 5 minutes at 2500 rpm, remove the supernatant, leave the sediment (5ml), add normal saline to 45ml, shake well;

[0062] The second centrifugation: 2500 rpm for 3 minutes, remove the supernatant, keep the sediment, add normal saline to 20ml, add 3.7g mannose oligosaccharides at the same time, shake well;

[0063] (5) Freeze-drying: Divide the purified fecal suspension into freeze-dried bottl...

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Abstract

The invention provides a coprophilous fungi positioning transplantation method. The method comprises the following steps: preparing capsules from coprophilous fungi serving as the content, orally taking the capsules, enabling the dissolved pH value of the capsule shells to adapt to the pH value of a target coprophilous fungi transplantation part, dissolving the capsules at the target transplantation part, and performing positioning transplantation on the coprophilous fungi at the target part. According to the method disclosed by the invention, the coprophilous fungi can be released at fixed points from the coprophilous fungi capsules according to different target transplantation parts according to the principle that different parts of human organs have different pH values, and the transplantation efficiency of the coprophilous fungi at the target transplantation part is improved.

Description

technical field [0001] The invention relates to the technical field of microbial medicine, in particular to a method for localized transplantation of fecal bacteria. Background technique [0002] "Fecal microbiota transplantation" (FMT) refers to the transplantation of functional flora in the feces of healthy people into the gastrointestinal tract of patients to rebuild new intestinal flora and achieve the treatment of intestinal and extraintestinal diseases. As a key salvage treatment for refractory inflammatory bowel disease (Crohn's disease, ulcerative colitis), fecal microbiota transplantation has achieved very good results. [0003] Since fecal bacteria transplantation uses live bacteria, fecal bacteria need to be transplanted to a specific diseased site for reproduction in order to be able to treat intestinal diseases. At present, the standardized fecal-bacteria transplantation is humanized separation of highly purified bacterial flora with the help of modern equipmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61K9/48A61P1/00A61P1/04
CPCA61K9/4858A61K35/741A61P1/00A61P1/04
Inventor 曾强赵鹏张晓莉杨婷婷
Owner GENERAL HOSPITAL OF PLA
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