Fermentation method of human intestinal flora and application
A technology of intestinal flora and fermentation method, which is applied in the fields of medicine and health care products, clinical and microbiology, and can solve problems such as unhealthy and affecting the effect of treatment
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Embodiment 1
[0033] A fermentation method of human intestinal flora, comprising the following steps:
[0034] (1) Screening of donors: select 3 boys aged 12-14 (2 12 years old, 1 13 years old), healthy, with no history of major diseases, no obvious gastrointestinal symptoms, and have not taken drugs for at least 6 months Any antibiotics, regular life.
[0035] (2) Sample collection and processing: collect fresh feces from each donor, quickly transfer to anaerobic workstation, weigh, add pre-reduced sterile saline at a ratio of 1:10 (w / v), vortex fully Spin and mix well; filter with double-layer gauze to collect feces suspension;
[0036] The pre-reduced sterile physiological saline is prepared from 0.9% sodium chloride solution, sterilized at 121° C. for 20 minutes, degassed at an anaerobic workstation and replaced with gas for more than 12 hours.
[0037] (3) Fermentation: Take 150 mL of fecal suspension from 3 donors, mix them evenly, dispense them into sterile serum bottles, 75 mL per...
Embodiment 2
[0044] A fermentation method of human intestinal flora, comprising the following steps:
[0045] (1) Screening of donors: select 3 boys aged 12-14 (2 12 years old, 1 13 years old), healthy, with no history of major diseases, no obvious gastrointestinal symptoms, and have not taken drugs for at least 6 months Any antibiotics, regular life.
[0046] (2) Sample collection and processing: collect fresh feces from each donor, quickly transfer to anaerobic workstation, weigh, add pre-reduced sterile saline at a ratio of 1:10 (w / v), vortex fully Spin and mix well; filter with double-layer gauze to collect feces suspension;
[0047] The pre-reduced sterile physiological saline is prepared from 0.9% sodium chloride solution, sterilized at 121° C. for 20 minutes, degassed at an anaerobic workstation and replaced with gas for more than 12 hours.
[0048] (3) Fermentation: Take 150 mL of fecal suspension from 3 donors, mix them evenly, dispense them into sterile serum bottles, 75 mL per...
Embodiment 3
[0052] A fermentation method of human intestinal flora, comprising the following steps:
[0053] (1) Screening of donors: A 12-year-old boy was selected, who was in good health, had no history of major diseases, had no obvious gastrointestinal symptoms, had not taken any antibiotics for at least 6 months, and had a regular life.
[0054] (2) Sample collection and processing: Collect the fresh feces of the donor, quickly transfer to the anaerobic workstation, weigh, add pre-reduced sterile saline at a ratio of 1:10 (w / v), and vortex fully. Evenly; filter with double-layer gauze to collect the feces suspension;
[0055] The pre-reduced sterile physiological saline is prepared from 0.9% sodium chloride solution, sterilized at 121° C. for 20 minutes, degassed at an anaerobic workstation and replaced with gas for more than 12 hours.
[0056] (3) Fermentation: Take 225 mL of the donor's fecal suspension, divide it into 3 sterile serum bottles, each bottle is 75 mL, and seal it with...
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