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Intestinal flora preparation capable of directionally proliferating and used for transplanting intestinal flora as well as preparation method and application of intestinal flora preparation

A technology of intestinal flora and production method, which is applied in microorganism-based methods, biochemical equipment and methods, and medical preparations containing active ingredients, etc. The effect of aggravating, relieving constipation, and solving the sparse number of flora

Pending Publication Date: 2022-03-15
MEI YI TIAN BIOLOGICAL MEDICINE WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, the dose of fecal microbiota transplantation is limited, so the number of functional flora in it is required to be higher than the normal level. At present, it is urgent to solve the problem of the proliferation of functional flora in the provided healthy human feces samples

Method used

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  • Intestinal flora preparation capable of directionally proliferating and used for transplanting intestinal flora as well as preparation method and application of intestinal flora preparation
  • Intestinal flora preparation capable of directionally proliferating and used for transplanting intestinal flora as well as preparation method and application of intestinal flora preparation
  • Intestinal flora preparation capable of directionally proliferating and used for transplanting intestinal flora as well as preparation method and application of intestinal flora preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Considering that the intestinal flora from the human body cannot be completely colonized in mice, resulting in inaccurate results of the determination of flora abundance, an in vivo simulation experiment was carried out in vitro, and human feces were taken as intestinal flora samples to observe Effect of aloe-emodin intervention on gut microbiota.

[0030] The feces collected come from healthy people. It is required not to smoke or drink alcohol, not to use antibiotics within half a year, and not to have various chronic diseases or infectious diseases.

[0031] The specific operation process is as follows:

[0032] 1. Fecal sample processing

[0033] (1) PBS buffer preparation

[0034] KH 2 PO 4 、Na 2 HPO 4 12H 2 O, NaCl and KCl are mixed in a mass ratio of 0.24:2.90:8.00:0.20 to obtain a mixture; then water is added to the mixture, and the pH value is adjusted with 0.1M HCl or NaOH to obtain a PBS buffer solution. The pH value of the PBS buffer solution is 7.57...

Embodiment 2

[0054] Adopt 2mL GAM medium with a concentration of 70g / L, 2mL of aloe-emodin solution with a concentration of 10g / mL, 1mL of PBS buffer solution, 1mL of intestinal microorganism solution with a mass concentration of 4%, and prepare aloe-emodin-GAM medium At the same time, through the replacement process of replacing oxygen for 11 hours, inoculate the intestinal microbial solution and cultivate it for 72 hours. Other conditions are the same as in Example 1, and the intestinal flora preparation is obtained by cultivation.

[0055] The same treatment method as in Example 1 was used for testing, and the results of the abundance determination of some intestinal flora are shown in Table 2.

Embodiment 3

[0057] Using 2 mL of GAM medium with a concentration of 50 g / L, 2 mL of aloe-emodin solution with a concentration of 9.93 g / mL, 1 mL of PBS buffer solution, and 0.5 mL of an intestinal microbial solution with a mass concentration of 6%, aloe-emodin-GAM culture During the preparation of the substrate, the oxygen was replaced for 13 hours, and the intestinal microbial solution was inoculated and cultivated for 84 hours. The other conditions were the same as in Example 1, and the intestinal flora preparation was obtained.

[0058] The same treatment method as in Example 1 was used for testing, and the results of the abundance determination of some intestinal flora are shown in Table 2.

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Abstract

The invention relates to a directionally proliferating intestinal flora preparation for intestinal flora transplantation and a preparation method and application thereof, the directionally proliferating intestinal flora preparation comprises a GAM culture medium, an aloe-emodin solution, a PBS buffer solution and an intestinal microbial solution in a volume ratio of 2: 2: 1: (0.5-1.5), the concentration of the aloe-emodin solution is 9.93-10.13 g / mL, and the mass concentration of the intestinal microbial solution is 4-6%. According to the method, the intestinal environment is simulated in vitro, the intestinal flora collected from excrement of a healthy human body is taken as a sample, the aloe-emodin solution is applied to promoting proliferation and fermentation of probiotics, a scientific basis is provided for clinical application, the probiotics are efficiently prepared and applied to coprophilous fungus transplantation, the state of the intestinal flora can be effectively adjusted, and the survival rate of coprophilous fungus transplantation is increased. And constipation, Crohn and other inflammations are relieved, so that the morbidity of colon cancer is reduced.

Description

technical field [0001] The invention relates to the field of fecal bacteria transplantation, in particular to an intestinal flora preparation for directional proliferation for intestinal flora transplantation, a preparation method and application thereof. Background technique [0002] Aloe-emodin is an anthraquinone compound. Mainly derived from Polygonaceae plant Rhubarb palmatum. Emodin-type anthraquinone compounds are active ingredients of many traditional Chinese medicines. They have various physiological and pharmacological activities such as antibacterial, anti-inflammatory, liver and kidney protection, inhibition of platelet aggregation, improvement of microcirculation, and anti-cancer. In terms of antibacterial properties, especially It is most sensitive to Staphylococcus and Streptococcus, and the effective concentration of antibacterial is 15-25ug / mL. Aloe-emodin has a two-way regulating effect on intestinal motility, and has a regulating effect on intestinal par...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/00A61P1/10A61P35/00A61K35/744A61K35/741A61K31/122C12R1/01C12R1/19C12R1/46C12R1/15
CPCC12N1/20A61K35/745A61K35/744A61K35/741A61K31/122A61P1/00A61P1/10A61P35/00A61K2300/00
Inventor 刘庆军沈鹤霄李国龙张帆刘慧敏周先锋熊云王峰
Owner MEI YI TIAN BIOLOGICAL MEDICINE WUHAN CO LTD
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