Preparation method of intestinal beneficial bacteria for decomposing dietary fibers and product

A technology of dietary fiber and beneficial bacteria, applied in the direction of bacteria, etc., can solve the problems of lack of beneficial bacteria products, and achieve the effect of expanding production and rapid preparation

Inactive Publication Date: 2018-09-14
知几未来(成都)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps create better ways to break down certain types of foods into useful ingredients like fibers called short-chain carbohydrates (SC). These SC have been shown to help improve digestion without causing stomach problems such as heartburn after eating them regularly. Additionally, this process makes it easier than previously possible to produce effective probiotic products containing these nutrients because they don't require expensive equipment during manufacturing processes.

Problems solved by technology

The technical problem addressed in this patented research relates how certain types of gut flora found within humans may help improve their overall wellness during physical activity without causing any negative side effects like constipating stool due to poor colonic motility caused by excessively high levels of glucose consumption.

Method used

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  • Preparation method of intestinal beneficial bacteria for decomposing dietary fibers and product

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Effect test

Embodiment 1

[0039] A preparation method for intestinal beneficial bacteria decomposing dietary fiber, comprising the steps of:

[0040] (1) Collect a small amount of feces from healthy volunteers and put them in a beaker, add sterile water to shake well, and filter out feces through filter paper;

[0041] (2) Pass through a filter device equipped with a 0.2 μm filter membrane, perform secondary filtration, and recover fecal bacteria onto the filter membrane;

[0042] (3) The intestinal microorganisms enriched on the filter membrane from feces bacteria are cut in half with sterilized scissors, put into 50mL, and the inulin culture solution with a concentration of 2% by weight, the filter membrane in (2) Add it into the culture solution, seal the culture solution, and then transfer it to a 37° constant temperature incubator and shake it on a shaker. After 24 hours, transfer the culture solution to a 50mL centrifuge tube, centrifuge at 8000rpm at high speed, discard the supernatant, and rec...

Embodiment 2

[0045] A preparation method for intestinal beneficial bacteria decomposing dietary fiber, comprising the steps of:

[0046] (1) Collect a small amount of feces from healthy volunteers and put them in a beaker, add sterile water to shake well, and filter out feces through filter paper;

[0047] (2) Pass through a filter device equipped with a 0.2 μm filter membrane, perform secondary filtration, and recover fecal bacteria onto the filter membrane;

[0048] (3) The intestinal microorganisms enriched on the filter membrane from feces bacteria are cut in half with sterilized scissors, put into 50mL inulin culture solution with a concentration of 5% by weight, and the filter membrane in (2) is added In the culture solution, seal the culture solution, and then transfer it to a 37° constant temperature incubator, and vibrate on a shaker. After 24 hours, transfer the culture solution to a 50mL centrifuge tube, centrifuge at 8000rpm at high speed, discard the supernatant, and recover ...

Embodiment 3

[0051] A preparation method for intestinal beneficial bacteria decomposing dietary fiber, comprising the steps of:

[0052] (1) Collect a small amount of feces from healthy volunteers and put them in a beaker, add sterile water to shake well, and filter out feces through filter paper;

[0053] (2) Pass through a filter device equipped with a 0.2 μm filter membrane, perform secondary filtration, and recover fecal bacteria onto the filter membrane;

[0054](3) The intestinal microorganisms that are enriched on the filter membrane from feces bacteria are cut in half with sterilized scissors, put into 50mL of inulin culture solution with a concentration of 10% by weight, and add the filter membrane in (2) In the culture solution, seal the culture solution, and then transfer it to a 37° constant temperature incubator, and vibrate on a shaker. After 24 hours, transfer the culture solution to a 50mL centrifuge tube, centrifuge at 8000rpm at high speed, discard the supernatant, and r...

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Abstract

The invention discloses a preparation method of intestinal beneficial bacteria for decomposing dietary fibers and a product and belongs to the technical field of preparation of probiotics. The preparation method of the intestinal beneficial bacteria for decomposing the dietary fibers, provided by the invention, comprises the following steps: (1) filtering coprophilous fungi for the first time andremoving manure dreg; (2) filtering for the second time to recycle the coprophilous fungi; (3) transferring the recycled coprophilous fungi into a culture solution; (4) oscillating through a shaking table, standing for one night and culturing; (5) centrifuging, removing supernatant and recycling the beneficial bacteria. According to the preparation method of the intestinal beneficial bacteria fordecomposing the dietary fibers, provided by the invention, a prepared product is a beneficial bacterium product which mainly aims at part of people with a relatively weak dietary fiber decomposing orabsorption capability, and related beneficial strains are screened from the coprophilous fungi of healthy people and are used for adjusting and maintaining an intestinal stable state of special people.

Description

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Claims

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Application Information

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Owner 知几未来(成都)生物科技有限公司
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