Application of coptis chinensis in preparation of product for promoting proliferation of intestinal beneficial bacteria
A technology for beneficial bacteria and intestinal flora imbalance, applied in the field of microorganisms, can solve problems such as no related reports, and achieve the effect of broadening the application field, promoting the proliferation of intestinal beneficial bacteria, and efficiently preparing
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[0041] Carry out in vivo simulation experiments in vitro, take human feces as intestinal flora samples, and observe the intervention effect of Coptis chinensis on intestinal flora. The specific operation process is as follows:
[0042] 1. Fecal sample processing
[0043] 10 g of stool samples were prepared with PBS buffer solution to a final concentration of 5%, and the mixed samples were sequentially passed through 20 mesh, 50 mesh, 100 mesh, and 200 mesh filters. Collect the filtrate into a centrifuge tube, put the centrifuge tube into a centrifuge, balance, centrifuge at 6000G, 4°C for 15min, and discard the supernatant. After the precipitate was weighed, the final concentration was 5% with PBS solution to obtain the intestinal microbial sample.
[0044] Wherein, the composition of PBS buffer comprises by concentration: KH 2 PO 4 0.24g / L, Na 2 HPO 4 12H 2 O 2.90g g / L, NaCl 8.00g g / L, KCl 0.20g g / L.
[0045] 2. Coptis chinensis intervention experiment
[0046] (1) P...
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