Coprophilous fungus cryoprotectant and application thereof in coprophilous fungus preservation

Abstract

The invention discloses a coprophilous fungus cryoprotectant and application thereof in coprophilous fungus preservation, and the coprophilous fungus cryoprotectant is prepared from the following components in percentage by mass: 20%-36% of trehalose, 14%-22% of cane sugar, 4%-8% of skim milk powder, 4%-8% of betaine, 0.02%-0.06% of L-cysteine hydrochloride, 0.3%-0.7% of D-sodium erythorbate, 0.1%-0.2% of glutathione and the balance of water. And the balance of sterile water. The coprophilous fungus cryoprotectant disclosed by the invention can effectively protect the initial composition proportion, flora diversity and bacterium survival rate of coprophilous fungus suspension under a low-temperature freezing condition. According to the coprophilous fungus cryoprotectant disclosed by the invention, the flora composition of a frozen bacterial suspension is closer to that of a bacterial suspension before freezing, and the coprophilous fungus cryoprotectant is of great significance for scientific researchers to accurately research intestinal flora composition.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cryoprotectant for fecal bacteria and its application in the preservation of fecal bacteria. Background technique [0002] The human gut is an extremely complex ecosystem, inhabited by about 1000 trillion anaerobic microorganisms. The flora in the intestinal tract facilitates people to absorb the nutrients in food, enhance the body's immunity, and maintain intestinal homeostasis. At present, there are two main research directions related to the human intestinal microflora. One is to establish accurate and efficient methods to identify the composition of the microbial community and analyze the proteomics and metabolomics of the intestinal flora. The relationship between changes in population homeostasis and human disease. The microflora in stool samples can better represent the composition of intestinal flora, so the analysis of stool samples is often used to study intestinal flor...

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Problems solved by technology

[0003] Low-temperature cryopreservation is recognized as a safe and effective method for long-term preservation of bacterial strains. At present, the commonly used cryoprotectant for fecal bacteria is 10% glycerol aqueous solution by volume fraction. Studies have shown that 10% glycerol aqueous solution has certain protection for fecal bacteria system. However, there are still some bacteria that cannot be well protected. The stool samples frozen in glycerin aqueous solution can not reflect the real intestinal flora. Microbes have a certain impact. Therefore, it is urgent to better protect the microbial diversity in fecal bacteria, reduce bacterial damage during freezing, better protect the integrity of fecal samples, and make fecal samples more truly reflect the intestinal tract. fecal bacteria cryoprotectant

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