Application of cynanchum auriculatum in preparation of product for promoting proliferation of intestinal beneficial bacteria
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A technology of psoriasis and beneficial bacteria applied in the field of microorganisms to achieve efficient preparation, broaden the application field, and promote the effect of proliferation
Pending Publication Date: 2022-03-25
MEI YI TIAN BIOLOGICAL MEDICINE WUHAN CO LTD
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However, its promoting effect on intestinal probiotics has not been reported so far
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preparation example Construction
[0039] Specifically, the preparation method of the fecal liquid comprises the following steps:
[0040] S1. Obtain a stool sample.
[0041] Feces are obtained from healthy donors, and the healthy donors in the present application include human or non-human animals; preferably, the non-human animals include any one of rats, chickens, pigs, cows, sheep and rabbits.
[0042] S2. Obtaining the original bacterial liquid.
[0043] Soak the stool sample in sterile water 5-10 times, and collect the supernatant after filtering. Filtration in this application may include operations such as membrane filtration or centrifugal filtration.
[0044] S3. Co-cultivation.
[0045] Place the original bacterial solution in the culture medium and cultivate it at 25-35°C for 3-4 days, centrifuge the culture to collect the bacterial cells, mix the bacterial cells with 5-10 times normal saline evenly, and obtain the fecal bacteria liquid; in the present application, the medium is GAM liquid medium ...
Embodiment
[0052] Human feces were taken as intestinal flora samples, and in vivo simulation experiments were carried out in vitro to observe the intervention effect of psoriasis on intestinal flora. The specific operation process is as follows:
[0053] 1. Fecal sample processing
[0054] Prepare 10 g of feces samples with PBS buffer solution to a final concentration of 5%, filter the mixed sample and centrifuge, discard the supernatant, weigh the precipitate, and use PBS solution to make a final concentration of 5% to volume , to obtain gut microbiome samples.
[0055] Wherein, the composition of PBS buffer comprises by concentration: KH 2 PO 4 0.24g / L, Na 2 HPO 4 12H 2 O 2.90g / L, NaCl 8.00g / L, KCl 0.20g / L.
[0056] 2. Intervention experiment of psoriasis
[0057] (1) Preparation of basal medium
[0058] Prepare the basal medium for cultivating intestinal microorganisms: GAM medium, containing 60g of modified GAM broth powder per 1000ml of ultrapure water.
[0059] The prepar...
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PUM
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Abstract
The invention provides application of cynanchum auriculatum in preparation of a product for promoting proliferation of intestinal beneficial bacteria, and relates to the technical field of microorganisms. According to the application, by simulating an intestinal environment in vitro and taking intestinal flora collected from faeces of a healthy human body as a sample, research finds that cynanchum auriculatum can remarkably promote proliferation of beneficial intestinal bacteria, particularly, the proliferation promotion effect on beneficial intestinal bacteria such as bifidobacteria, faecobacteria and Rosella is extremely remarkable, and in addition, the application has the advantages that the effect of promoting proliferation of the beneficial intestinal bacteria is obvious; the invention further provides a method for applying cynanchum auriculatum to directional fermentation of the specific donor flora for coprophilous fungus transplantation, so that the specific donor flora beneficial to the intestinal tract is obtained through directional fermentation, the specific donor flora can enter the gastrointestinal tract of a patient through coprophilous fungus transplantation, a new intestinal flora is reconstructed, and the effect of improving the intestinal tract is achieved. According to the application, a scientific basis is provided for clinical application, and the application field of cynanchum auriculatum is expanded.
Description
technical field [0001] The invention relates to the technical field of microbes, in particular to the application of psoriasis auris in the preparation of products for promoting the proliferation of intestinal beneficial bacteria. Background technique [0002] Cynanchum auriculatum Royle ex Wight is a plant of the genus Royle ex Wight in the family Ascariaceae, widely distributed in Sichuan, Guizhou, Yunnan and other places. Contains a variety of nutrients and biologically active substances, it has the functions of nourishing liver and kidney, strengthening muscles and bones, benefiting essence and blood, invigorating spleen and eliminating food, detoxifying and treating sores. Studies have shown that psoriasis has the functions of scavenging free radicals, improving specific and non-specific immunity, inhibiting tumors, lowering cholesterol, and reducing myocardial oxygen consumption. However, there is no related report about its promoting effect on intestinal probiotics. ...
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