Method for establishing hypertension yin-deficiency syndrome and/or hypertension non-yin-deficiency syndrome animal model by coprophilous fungus transplantation method, evaluation and application

Abstract

The invention relates to a construction method of an animal model, in particular to a construction method of an animal model, and particularly relates to a method for establishing a hypertension yin-deficiency syndrome and / or hypertension non-yin-deficiency syndrome animal model by applying a coprophilous fungus transplantation method, an evaluation method and an application of the hypertension yin-deficiency syndrome and / or hypertension non-yin-deficiency syndrome animal model. A corresponding animal model with the hypertension yin deficiency syndrome and / or non-yin deficiency syndrome is obtained by preparing coprophilous bacteria liquid of a patient with the hypertension yin deficiency syndrome and / or non-yin deficiency syndrome and transplanting the prepared coprophilous bacteria liquid after a mouse takes a quadruple antibiotic drug in a drenching and drinking manner, and meanwhile, animal model evaluation is performed by adopting multiple methods to confirm whether the model is successfully established or not. The invention provides the hypertension yin-deficiency syndrome and / or non-yin-deficiency syndrome animal model which is high in stability, easy to popularize, good in repeatability, high in success rate, economical and practical, a basis is provided for research on traditional Chinese medicine treatment of hypertension, and a thought is provided for standardization and normalization of yin-deficiency syndrome model evaluation.

Description

technical field [0001] The invention belongs to a method for constructing an animal model, in particular to a method for establishing an animal model of hypertension with yin deficiency syndrome and / or hypertension with non-yin deficiency syndrome by using a fecal bacteria transplantation method, evaluation and application. Background technique [0002] Hypertension is a common clinical disease, frequently-occurring disease, and the basis of cardiovascular and cerebrovascular diseases. About 95% of patients, whose etiology is unknown, is called "essential hypertension". Essential hypertension, also known as hypertension, is a clinical syndrome mainly manifested by increased systemic arterial blood pressure; and systemic diseases caused by the interaction of multiple risk factors. Among them, in addition to the symptoms of hypertension itself, long-term hypertension can also affect the functions of the heart, brain, and kidney, which can eventually lead to the failure of the...

AI Technical Summary

Problems solved by technology

[0005] At present, the mouse model of fecal transplantation test is not only cumbersome to construct, the model effect is poor, and it is difficult to reflect the real situation; and there is no report on the establishment of animal models of hypertension with yin deficiency syndrome and / or hypertension with non-yin deficiency syndrome through feces transplantation

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