Coprophilous fungi cryopreservation fluid and preservation method thereof

Abstract

The invention relates to the technical field of medical treatment, and particularly relates to a coprophilous fungi cryopreservation fluid and a preservation method thereof. The cryopreservation fluidcontains the following components in weight percentage by compounding: 1-5% of glycerin, 1-3% of tryptone, 0.1-0.5% of vitamin B, 0.7-0.9% of normal saline, and the balance of coprophilous fungi extracting soluiont. The coprophilous fungi cryopreservation fluid is cooled in match with a program, to control the coprophilous fungi cooling speed and time, so that the preservation time of the coprophilous fungi fluid at 80 DEG C is as long as more than 2 years, which is far higher than the storage time of coprophilous fungi whcih is 3-6 months in a general laboratory; and therefore, a solid foundation is laid for the storage of coprophilous fungi in a laboratory and the establishment of a coprophilous fungi specimen library.

Description

Technical field [0001] The invention relates to the field of medical technology, in particular to a cryopreservation liquid of fecal bacteria and a storage method thereof. Background technique [0002] Fecal bacteria transplantation technology refers to the separation of functional bacteria from the feces of healthy people, and transplant them into the patient’s intestine through gastrointestinal endoscopes, colonoscopy, enema, etc., to rebuild the patient’s intestinal flora and achieve intestinal and extra-intestinal diseases The technique of treatment. As early as 1700 years ago, during the Eastern Jin Dynasty, Ge Hong wrote in his "Elbow Reserve Emergency Recipe" that feces can treat food poisoning and diarrhea. Li Shizhen's "Compendium of Materia Medica" also records the use of Huanglong Tang (feces) to treat diseases. A case of fecal bacteria transplantation that can cure diseases was first reported in foreign countries in 1985. Eiseman and others treated patients with pse...

AI Technical Summary

Problems solved by technology

[0004] There are many ways to preserve the feces liquid, but these methods have certain defects: these methods either cannot preserve the feces liquid for a long time, or a large number of bacteria are inactivated after the feces liquid is preserved

For example, the fecal bacteria liquid is directly frozen and stored in a -80°C refrigerator. In this method, without the protection of the protective solution, the bacteria will be "frozen" by the ice crystals during the rapid cooling process, resulting in a decline in the bacterial viability. After a certain period of time, the bacteria There will also be a large number of deaths, the type and abundance of bacteria in the feces will decrease, and the inactivation of some bacterial flora will cause the effect of fecal bacteria liquid in FMT.

In the prior art, there are also substances such as EDTA salt and sodium citrate, and the preservation solution prepared with water and ethanol as solvents can preserve the fecal samples for a week in a sealed room temperature, but this method is not suitable for medium and long-term preservation of fecal bacteria, and this The fecal liquid preserved by this method needs to be centrifuged before the fecal transplant to remove some components in the protective solution. In this way, the beneficial components in the bacterial liquid will also be lost, thereby reducing the efficacy of the fecal liquid.