Coprophilous fungi cryopreservation fluid and preservation method thereof

A technology of cryopreservation solution and preservation method, which is applied in the field of fecal bacteria cryopreservation solution and its preservation, which can solve the problems of reducing the efficacy of fecal bacteria solution, the decline of bacterial viability, and the death of bacteria, so as to protect the activity of microorganisms, maintain biological activity, Preserve Significant Effects

Inactive Publication Date: 2018-12-07
卓源健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many ways to preserve the feces liquid, but these methods have certain defects: these methods either cannot preserve the feces liquid for a long time, or a large number of bacteria are inactivated after the feces liquid is preserved
For example, the fecal bacteria liquid is directly frozen and stored in a -80°C refrigerator. In this method, without the protection of the protective solution, the bacteria will be "frozen" by the ice crystals during the rapid cooling process, resulting in a decline in the bacterial viability. After a certain period of time, the bacteria There will also be a large number of deaths, the type and abundance of bacteria in the feces will decrease, and

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Before feces collection, the collection box is fully sterilized. After the feces are collected in the collection box, the collection box is immediately sealed with a sealed bag and the air in the sealed bag is exhausted to maintain the anaerobic state of the feces. After the sealing is completed, it is quickly transported back to the laboratory, and then agitation, centrifugation, resuspension, filtration and other steps are performed to achieve rapid separation.

[0026] Add 2% glycerol, 1% tryptone, 0.2% vitamin B, and 0.7% normal saline to the obtained fresh fecal bacteria extract to prepare a preservation solution. Use a program cooling device to set the cooling conditions: the cavity temperature is from normal Decrease at a rate of 10°C per minute, and maintain it for half an hour when it drops to 4°C at a constant rate, then cool to -20°C at a rate of 5°C per minute for one hour, and then cool to -80°C at a rate of 1°C per minute. After two hours, the frozen fecal ba...

Embodiment 2

[0028] Before feces collection, the collection box is fully sterilized. After the feces are collected in the collection box, the collection box is immediately sealed with a sealed bag and the air in the sealed bag is exhausted to maintain the anaerobic state of the feces. After the sealing is completed, it is quickly transported back to the laboratory, and then agitation, centrifugation, resuspension, filtration and other steps are performed to achieve rapid separation.

[0029] The obtained fresh fecal bacteria extract was added with 5% glycerol, 3% tryptone, 0.3% vitamin B, and 0.8% normal saline to prepare a preservation solution. Use a program cooling instrument to set the cooling conditions: the cavity temperature is from normal temperature to every Decrease at a rate of 10°C per minute, and maintain it for half an hour when it drops to 4°C at a constant rate, then cool to -20°C at a rate of 5°C per minute for one hour, and then cool to -80°C at a rate of 1°C per minute. Aft...

Embodiment 3

[0031] Before feces collection, the collection box is fully sterilized. After the feces are collected in the collection box, the collection box is immediately sealed with a sealed bag and the air in the sealed bag is exhausted to maintain the anaerobic state of the feces. After the sealing is completed, it is quickly transported back to the laboratory, and then agitation, centrifugation, resuspension, filtration and other steps are performed to achieve rapid separation.

[0032] Add 3% glycerol, 2% tryptone, 0.3% vitamin B, and 0.9% saline to prepare the obtained fresh fecal bacteria extract to prepare a preservation solution. Use a program cooling instrument to set the cooling conditions: the cavity temperature is from normal temperature to every Decrease at a rate of 10°C per minute, and maintain it for half an hour when it drops to 4°C at a constant rate, then cool to -20°C at a rate of 5°C per minute for one hour, and then cool to -80°C at a rate of 1°C per minute. After two ...

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PUM

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Abstract

The invention relates to the technical field of medical treatment, and particularly relates to a coprophilous fungi cryopreservation fluid and a preservation method thereof. The cryopreservation fluidcontains the following components in weight percentage by compounding: 1-5% of glycerin, 1-3% of tryptone, 0.1-0.5% of vitamin B, 0.7-0.9% of normal saline, and the balance of coprophilous fungi extracting soluiont. The coprophilous fungi cryopreservation fluid is cooled in match with a program, to control the coprophilous fungi cooling speed and time, so that the preservation time of the coprophilous fungi fluid at 80 DEG C is as long as more than 2 years, which is far higher than the storage time of coprophilous fungi whcih is 3-6 months in a general laboratory; and therefore, a solid foundation is laid for the storage of coprophilous fungi in a laboratory and the establishment of a coprophilous fungi specimen library.

Description

Technical field [0001] The invention relates to the field of medical technology, in particular to a cryopreservation liquid of fecal bacteria and a storage method thereof. Background technique [0002] Fecal bacteria transplantation technology refers to the separation of functional bacteria from the feces of healthy people, and transplant them into the patient’s intestine through gastrointestinal endoscopes, colonoscopy, enema, etc., to rebuild the patient’s intestinal flora and achieve intestinal and extra-intestinal diseases The technique of treatment. As early as 1700 years ago, during the Eastern Jin Dynasty, Ge Hong wrote in his "Elbow Reserve Emergency Recipe" that feces can treat food poisoning and diarrhea. Li Shizhen's "Compendium of Materia Medica" also records the use of Huanglong Tang (feces) to treat diseases. A case of fecal bacteria transplantation that can cure diseases was first reported in foreign countries in 1985. Eiseman and others treated patients with pse...

Claims

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Application Information

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IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 张彬郭建姜秋金泽宇吴亮姜晶
Owner 卓源健康科技有限公司
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