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Tea residue fermentation broth and preparation method and application thereof

A technology of fermentation liquid and tea dregs, which is applied in the field of tea dregs fermentation liquid and its preparation

Inactive Publication Date: 2019-11-26
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the microbial fermentation of tea dregs as a medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of tea dregs hydrolyzate:

[0036] Put 100g of tea residue in a 2L round bottom flask, then add 1000mL of 0.5wt% H 2 SO 4 The solution was mixed evenly, sealed with gauze and kraft paper, and treated in a high-pressure steam sterilizer at 121° C. for 60 minutes to obtain a reaction solution. After the reaction liquid is cooled, squeeze the tea dregs with gauze in an ultra-clean bench, and filter to obtain a filtrate. Adjust the pH of the filtrate to 7.0 with calcium hydroxide solid, dispense it into sterilized 50mL centrifuge tubes, centrifuge at 12000rpm for 5min, then transfer the supernatant to a sterile culture bottle to obtain a tea dregs hydrolyzate, and store it at 4°C until use.

[0037] Preparation of tea dregs hydrolyzate medium:

[0038] In the tea dregs hydrolyzate, add inorganic salt (0.60wt% KH 2 PO 4 , 0.19 wt% K 2 HPO 4 , 0.28 wt% MgCl 2 , 0.15wt% FeSO 4 ·7H 2 O and 0.12wt% ZnSO 4 ) to obtain the tea residue hydrolyzate medium....

Embodiment 2

[0047] Except maintaining the total carbon-to-nitrogen ratio (C / N) of the tea dregs hydrolyzate medium in the fermentation process at about 200:1, other conditions were the same as in Example 1.

Embodiment 3

[0049] Except that the total carbon-to-nitrogen ratio (C / N) of the tea dregs hydrolyzate medium was maintained at about 100:1 during the fermentation process, other conditions were the same as in Example 1.

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Abstract

The invention discloses a tea residue fermentation broth and preparation method and application thereof. The preparation method comprises the steps that (1) tea residue ishydrolyzedto obtain a tea residue hydrolysate; (2) rhodosporidium toruloides is activated in a YEPD medium to obtain the activated rhodosporidium toruloides; (3) the activated rhodosporidium toruloidesis inoculated and cultured in the tea residue hydrolysate, and bacterial cells are collected after separation; (4) the bacterial cells are inoculated into the tea residue hydrolysate culture medium for expandedfermental cultivation; and the tea residue hydrolysate medium contains the tea residue hydrolysate and inorganic salts. According to the tea residue fermentation broth and the preparation method and application thereof,the tea residue hydrolysateand therhodosporidium toruloideswhich are cultured by the tea residue hydrolysate and capable of being directly used as feed additives for laying henscan be obtained,.

Description

technical field [0001] The invention relates to a tea dregs fermented liquid and its preparation method and application. Background technique [0002] Microbial oils are saturated or unsaturated fats synthesized intracellularly or extracellularly by special oil-producing microorganisms (bacteria, yeast, mold, or algae, etc.) using carbohydrates, hydrocarbons, and ordinary oils as carbon sources under appropriate conditions. Saturated triglycerides. Compared with the traditional oil production process, the microbial oil production process has many advantages: (1) microbial cell proliferation is fast, the production cycle is short, and the oil content is high; (2) the substrate raw materials required for microbial growth and metabolism have a wide range of sources (such as starch, sugar, cellulose, food industry waste), and the price is cheap; (3) microbial oil requires less labor than traditional oil production, and the production process is not limited by factors such as si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/18A23K10/26A23K20/20C12N1/16C12R1/645
CPCA23K10/12A23K10/26A23K10/18A23K20/20C12N1/16
Inventor 祁峰黄建忠翁雪清陈建龙赵燕玉沈培杰
Owner FUJIAN NORMAL UNIV
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