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Strain for co-producing unsaturated fatty acid and carotenoid and application thereof

A technology of unsaturated fatty acids and carotene, applied in the field of bacterial strains that co-produce unsaturated fatty acids and carotenoids, can solve the problems of chemical reagents remaining on the bacteria, discounts on the reuse value of bacteria residues, etc., and achieve great social significance and Economic value, strong tolerance effect

Active Publication Date: 2021-08-27
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some of these wall-breaking methods are only suitable for a small amount of crushed oil extraction in the laboratory. If a large amount of industrial production is carried out, the economic cost is a major factor that cannot be ignored. Although some have achieved the effect of breaking the bacterial wall, their chemical reagents remain On the bacteria, the reuse value of the bacteria residue is greatly reduced

Method used

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  • Strain for co-producing unsaturated fatty acid and carotenoid and application thereof
  • Strain for co-producing unsaturated fatty acid and carotenoid and application thereof
  • Strain for co-producing unsaturated fatty acid and carotenoid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: This example illustrates the screening method of Rhodosporidium toruloides.

[0028] The original strain of Rhodosporidium toruloides was a wild fungus screened from the soil collected from the lake near the Xiyuan cafeteria of Nanjing University of Technology;

[0029] Specifically, the YPD plate is used as the culture medium, cultured under aerobic conditions at 30°C, cultured for 2-4 days, streaked and purified 5-7 times, and finally the strains that can grow on the plate are verified, and their Fermentation, investigation of its fermentation products and performance, found that the strain can use a lot of carbon sources for growth, and can produce trace amounts of carotenoids. This strain was used as the starting strain for strain transformation.

[0030] The method for performing the first step of ion beam mutagenesis is as follows:

[0031] Use the original strain of Rhodosporidium toruloides for activation culture, culture temperature 30°C, 100mL sha...

Embodiment 2

[0048] Example 2: This example illustrates the passage stability of mutant strains Z11 and Z12.

[0049] The passage stability of mutant strains Z11 and Z12 was detected in the fermentation medium with glucose as carbon source. The subculture fermentation test results of strains Z11 and Z12 are shown in Table 2:

[0050] Table 2 strains Z11 and Z12 subculture fermentation test results

[0051]

[0052] It can be seen from the experimental results that after 7 consecutive passages, the carotenoid production of the two mutant strains is relatively stable, and they have good passage stability, which can be used as production strains for further research and development.

Embodiment 3

[0053] Example 3: This example illustrates the process of fermenting and producing carotenoids from Rhodosporidium toruloides Z11.

[0054] The culture medium formula described in the present embodiment (% is mass percent):

[0055] Plate medium: yeast powder 1%, peptone 2%, glucose 2%, agar 2%, the rest is water, pH 6.

[0056] Seed medium: 1% yeast powder, 2% peptone, 2% glucose, and the rest is water.

[0057] Fermentation medium: glucose 8%, K 2 HPO 4 0.04%, NaH 2 PO 4 0.1%, MgSO 4 ·H 2 O 0.15%, yeast extract 0.175%, the trace elements are: ZnSO 4 , CaCl 2 , MnCl 2 、CuSO 4 are 0.192×10 -7 g / L, 0.167g / L, 0.54×10 -4 g / L, 0.16×10 -4 g / L.

[0058] Rhodosporidium toruloides Z11 was inoculated on the plate medium for aerobic culture, the culture temperature was 30° C., and the culture time was 26 hours. Inoculate the Z11 cultured on the plate into the seed medium, the liquid volume in a 250mL Erlenmeyer flask is 50mL, the culture temperature is 30°C, and the cult...

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Abstract

The invention relates to a strain for co-production of unsaturated fatty acid and carotenoid and an application of the strain. The strain is classified and named as Rhodosporidium toruloides Z11, and the preservation number of the strain is CCTCC NO: M 2021226. According to the invention, through ion beam mutagenesis, the obtained mutant strain takes glucose as a unique carbon source, and the grease content and carotenoid yield in shake flask fermentation respectively reach 66% and 13.5 mg / g, which are respectively 2.12 times and 12.85 times of those of an original strain cultured under the same condition; the strain is high in stability, high in product yield and good in repeatability, and is an excellent strain suitable for being used as animal feed. The strain is rich in a large amount of protein and a certain amount of vitamin carotenoid, as an animal additive, the preparation process is simple, the production raw material source is wide, large-scale production is facilitated, the problem that residual mushroom dregs after microbial oil extraction are not suitable for animals to digest and absorb is solved, meanwhile the production cost of the microbial oil is reduced, and comprehensive utilization of resources is promoted.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and relates to a bacterial strain co-producing unsaturated fatty acid and carotenoid and application thereof. Background technique [0002] Nowadays, with the development of the economy, the quality of human life has been greatly improved, and a healthy diet has been advocated, which has made rapid progress in the animal husbandry and breeding industries. If the traditional method of feeding is used, the protein obtained cannot reach high-quality grades. Use bacterial protein as a substitute to solve the shortage of protein feed supply. [0003] Cell protein, also known as microbial protein, usually refers to the cell protein obtained by cultivating single-cell microorganisms. It is a microbial cell artificially cultivated by many industrial and agricultural wastes and petroleum wastes. Therefore, single-cell protein is not a pure protein, but a cytoplasmic mass composed of a mix...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P23/00C12P7/64A23K10/16C12R1/645
CPCC12N1/16C12P23/00C12P7/64A23K10/16Y02E50/10
Inventor 信丰学章晓宇姜岷李甲文汤韵涵章文明蒋雨佳
Owner NANJING UNIV OF TECH
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