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Rhodosporidium toruloides Y0 and application thereof

A technology of yeast and rhodosporidium, applied in the fields of application, fungi, animal feed, etc., can solve problems such as ineffective utilization of breeding waste

Active Publication Date: 2014-07-09
广州诺晶生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although some aquaculture wastewater treatment methods separate the aquaculture waste from the water body, they cannot effectively utilize the aquaculture waste

Method used

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  • Rhodosporidium toruloides Y0 and application thereof
  • Rhodosporidium toruloides Y0 and application thereof
  • Rhodosporidium toruloides Y0 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Isolation, screening and identification of Rhodosporidium toruloides Y0.

[0021] (1) Isolation and screening of Rhodosporidium toruloides Y0

[0022] Take 1g of sludge from the drainage outlet of the prawn farm in Zhanjiang, Guangdong, add 10ml of sterilized seawater, mix well, and take 200μL of yeast isolation medium (YPD) plate, YPD is a special medium for yeast, and the preparation method is: glucose (individually sterilized Bacteria) 20g, peptone 20g, yeast extract 10g, seawater 1L, after sterilization, add 1ml of 50mg / ml chloramphenicol after cooling. After culturing at 30°C for 48 hours, the growing colonies were picked, purified and preserved to obtain isolated bacteria.

[0023] Cultivate the above-mentioned isolated bacteria in liquid YPD, take 1 μL and inoculate them on the yeast screening medium, which is a prawn culture waste medium plate, and the preparation method is: 20g of dried shrimp feces powder, 20g of agar powder, 1L of seawater, Cool slightly...

Embodiment 2

[0048] Rhodosporidium toruloides Y0 was inoculated in YPD medium and cultured overnight at 30°C as a seed culture solution. The wastewater discharged from the central sewage system of the intensive shrimp farming system is collected into the resource treatment pool. After 24 hours of natural settlement, the excess water in the upper layer is sucked up by the water pump, leaving only a small amount of sewage and residual bait feces at the bottom. Introduce ozone for disinfection treatment. After detoxification treatment, place it for 24 hours. During this period, stir the pool water several times to help the aeration of the water body. Add the seed culture solution at a ratio of 5% (V / V), then add 14‰ sucrose according to the mass-volume ratio, stir and mix well, and ferment at room temperature for 48 hours, stirring as many times as possible during the period.

[0049] After the fermentation is finished, stir the pool water, and pump out the pool water respectively to pass thr...

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Abstract

The invention discloses Rhodosporidium toruloides Y0 and application thereof. The Rhodosporidium toruloides Y0 is preserved in China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan City, China, on December 29, 2013, with an accession number of CCTCC No. M2013725. The Rhodosporidium toruloides Y0 has abundant nutrients, is applicable to production of functional bait additives for animals and can rapidly grow in mariculture waste water with mariculture waste water as a main nutriment source and 1 to 1.4% of cane sugar added according to a massic volume ratio as a supplementary carbon source; so the purposes of utilization of mariculture waste water, reduction of waste water discharge pollution and obtainment of high-value bait additives for aquatic livestock are achieved.

Description

Technical field: [0001] The invention belongs to the technical field of microbial application, and in particular relates to Rhodosporidium toruloides Y0 and its application. Background technique: [0002] Yeast is rich in nutrients and moderate in size. It is an excellent natural bait for many organisms. At the same time, yeast is also a type of saprophytic bacteria that can decompose various substances and use organic matter, ammonia nitrogen, hydrogen sulfide and other aquaculture waste in the aquaculture water to form dominant populations, effectively It can effectively inhibit the growth of other harmful microorganisms and play the role of biological control. In recent years, with the advancement of aquaculture technology, the application of yeast in aquaculture has become more and more extensive. [0003] However, there are still very few yeasts that can be used in seawater farming in the market. The commonly used yeast is still baker's yeast (Saccharomyces cerevisiae)...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23K1/16A23K1/18C02F3/34C12R1/645C02F103/08A23K10/18A23K50/80
Inventor 罗鹏江海英夏建军胡超群
Owner 广州诺晶生物技术有限公司
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