3 results about "Galactokinase" patented technology

The application discloses recombinant saccharomyces cerevisiae for producing wax melia oil and a construction method and application thereof, and belongs to the technical field of biology. By modular integration and optimization of terpene precursor supply and gene expression, the galactokinase genes GAL1, GAL7 and GAL10 are knocked out in the original saccharomyces cerevisiae, and terpene precursor synthesis genes and wax melia oil characteristic terpene synthesis enzyme genes are introduced, so that the recombinant saccharomyces cerevisiae is obtained, that is, a wax melia oil production strain. The proportion of elemol in the fermentation product of the recombinant strain is as high as 51.0%, and six target terpene components are determined. The application provides a stable biological product strain for the application of wax melia oil in the fields of spices, cosmetics and medicines, and has significant economic value and practical application value.

This invention provides a method for seamless editing of a double-copy target gene in a bacterial artificial chromosome. The method includes: first, constructing a bacterial artificial chromosome containing a double-copy target gene and introducing it into *E. coli* SW102 to obtain a first recombinant bacterium; second, constructing a target fragment including a chloramphenicol resistance gene, a gentamicin resistance gene, and a galactokinase encoding gene, contacting this target fragment with the first recombinant bacterium, and obtaining a second recombinant bacterium through a first homologous recombination; finally, constructing a target fragment for editing the target gene and obtaining a third recombinant bacterium through a second homologous recombination, thus completing the seamless editing of the double-copy gene. The method provided by this invention can simultaneously recombine and modify a double-copy target gene in a bacterial artificial chromosome, avoiding the problems of low double-site knock-in rate and high screening difficulty associated with single galactokinase encoding gene marker screening, and achieving seamless editing of the double-copy target gene.

The invention discloses recombinant saccharomyces cerevisiae for producing chimonanthus praecox essential oil as well as a construction method and application thereof, and belongs to the technical field of biology. Through modular integration and optimization of terpene precursor supply and gene expression, galactokinase genes GAL1, GAL7 and GAL10 are knocked out from starting strains of saccharomyces cerevisiae, and terpene precursor synthetic genes and chimonanthus praecox essential oil characteristic terpene synthase genes are introduced to obtain recombinant saccharomyces cerevisiae, namely the chimonanthus praecox essential oil production strains. The proportion of elemol in the fermentation product of the recombinant strain is as high as 51.0%, and six target terpenoids are clarified. The invention provides a stable production variety for the application of the chimonanthus praecox essential oil in the fields of spices, cosmetics and medicines, and has remarkable economic value and practical application value.