5 results about "Galactokinase" patented technology

The application discloses recombinant saccharomyces cerevisiae for producing wax melia oil and a construction method and application thereof, and belongs to the technical field of biology. By modular integration and optimization of terpene precursor supply and gene expression, the galactokinase genes GAL1, GAL7 and GAL10 are knocked out in the original saccharomyces cerevisiae, and terpene precursor synthesis genes and wax melia oil characteristic terpene synthesis enzyme genes are introduced, so that the recombinant saccharomyces cerevisiae is obtained, that is, a wax melia oil production strain. The proportion of elemol in the fermentation product of the recombinant strain is as high as 51.0%, and six target terpene components are determined. The application provides a stable biological product strain for the application of wax melia oil in the fields of spices, cosmetics and medicines, and has significant economic value and practical application value.

This invention provides a method for seamless editing of a double-copy target gene in a bacterial artificial chromosome. The method includes: first, constructing a bacterial artificial chromosome containing a double-copy target gene and introducing it into *E. coli* SW102 to obtain a first recombinant bacterium; second, constructing a target fragment including a chloramphenicol resistance gene, a gentamicin resistance gene, and a galactokinase encoding gene, contacting this target fragment with the first recombinant bacterium, and obtaining a second recombinant bacterium through a first homologous recombination; finally, constructing a target fragment for editing the target gene and obtaining a third recombinant bacterium through a second homologous recombination, thus completing the seamless editing of the double-copy gene. The method provided by this invention can simultaneously recombine and modify a double-copy target gene in a bacterial artificial chromosome, avoiding the problems of low double-site knock-in rate and high screening difficulty associated with single galactokinase encoding gene marker screening, and achieving seamless editing of the double-copy target gene.

The invention belongs to the technical field of biology, and discloses red algae oligosaccharide with a bowel relaxing function, a composition, a preparation method and application. The compound enzyme agent (obtained by compounding red algae polysaccharide enzyme, galactokinase and galactosyltransferase) in the composition for preparing red algae oligosaccharide provided by the invention can directly take a red algae coarse material subjected to simple treatment as a fermentation substrate, and meanwhile, Zn < 2 + > with a specific concentration in a fermentation solution is coordinated with the compound enzyme agent; the yield of the red algae oligosaccharide obtained through fermentation is increased, and the content of the red algae oligosaccharide with the polymerization degree of 4-6 is 95% or above; when the composition is applied to preparation of red algae oligosaccharide, the composition has the advantages of low substrate purity requirement, high product polymerization degree uniformity, high yield and the like, and has a good application prospect.

The invention discloses recombinant saccharomyces cerevisiae for producing chimonanthus praecox essential oil as well as a construction method and application thereof, and belongs to the technical field of biology. Through modular integration and optimization of terpene precursor supply and gene expression, galactokinase genes GAL1, GAL7 and GAL10 are knocked out from starting strains of saccharomyces cerevisiae, and terpene precursor synthetic genes and chimonanthus praecox essential oil characteristic terpene synthase genes are introduced to obtain recombinant saccharomyces cerevisiae, namely the chimonanthus praecox essential oil production strains. The proportion of elemol in the fermentation product of the recombinant strain is as high as 51.0%, and six target terpenoids are clarified. The invention provides a stable production variety for the application of the chimonanthus praecox essential oil in the fields of spices, cosmetics and medicines, and has remarkable economic value and practical application value.

The invention relates to the field of bioengineering and microbial fermentation, and discloses a recombinant bacterium with high nucleoside yield as well as a construction method and application of the recombinant bacterium. The invention specifically provides bacillus subtilis or bacillus amyloliquefaciens, and the nucleoside production capability of the strain is enhanced compared with that of an unmodified strain by enhancing a gene galK encoding galactokinase in a microorganism. The enhancement mode is to mutate the galK gene and / or enhance the promoter sequence, and the nucleoside production capacity of the obtained strain is improved to different degrees. An effective means is provided for large-scale production of nucleoside, and the application prospect is wide.