36 results about "Galactokinase" patented technology

Disclosed are inhibitors of human galactokinase of formula (1) that are useful in treating or preventing a galactokinase mediated disease or disorder, e.g., galactosemia. Also disclosed are a composition comprising a pharmaceutically acceptable carrier and at least one inhibitor of the invention, and a method of treating or preventing such disease or disorder in a mammal. Formula (I).

The invention relates to application of galactokinase in synthesizing N-acetylgalactose-1-phosphoric acid and derivatives thereof, belonging to the technical field of biotechnology. The application method comprises the following steps: (1) preparing a galactokinase solution from galactokinase of which the amino acid sequence is disclosed as SEQ ID No.1; (2) preparing an N-acetylgalactose solution or N-acetylgalactose derivative solution from N-acetylgalactose or N-acetylgalactose derivatives; and (3) proportionally mixing the galactokinase solution with the N-acetylgalactose solution or N-acetylgalactose derivative solution, adding MgCl2 and ATP (adenosine triphosphate), reacting, and purifying after the reaction, thereby obtaining the N-acetylgalactose-1-phosphoric acid or derivatives thereof. The invention overcomes the technical defect that the galactokinase can not be applied to actual production since the galactokinase can not be synthesized into N-acetylgalactose-1-phosphoric acid or the synthesis efficiency of N-acetylgalactose-1-phosphoric acid is very low in the prior art.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on galactose kinase, acetyl-CoA carboxylase, pyruvate dehydrogenase The series of catalytic reactions are completed, and the present invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention discloses a bovine lactoferricin peptide constitutive type yeast expression vector and a preparation method thereof. The expression vector is obtained by introducing a bovine lactoferricin peptide gene into pYES2 plasmids and utilizing a TPI (triosephosphate isomerase) promoter to substitute a GAL1 (galactokinase 1) promoter of the pYES2 plasmids. The pYES2-TPI-LfcinB recombinant plasmids constructed by the preparation method disclosed by the invention can effectively and stably secrete bovine lactoferricin peptide without using an inducer, so that production cost and production complexity are lowered.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on galactose kinase, carbamoyl phosphate synthase, hydrogen cyanide synthase The series of catalytic reactions are completed, and the present invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on galactose kinase, pyruvate carboxylase, malonate semialdehyde desorption The series of catalyzed reactions of the hydrogenase is completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on galactose kinase, carbamoyl phosphate synthetase, malate dehydrogenase The series of catalytic reactions are completed, and the present invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on the development of galactose kinase, urea carboxylase, and hydrogen cyanide synthase. The series of catalytic reactions are completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention discloses a fluorescent probe for analyzing, testing and screening a galactokinase inhibitor. The structure of the fluorescent probe is shown in the formula (I) in the specification, wherein X is O or NH, and R2 is selected from compounds as shown in the specification. The fluorescent probe can simplify the conventional screening process of the galactokinase inhibitor and is directly used in any cell system expressing galactokinase without use of purified and refined galactokinase protein; with the application of the fluorescent probe, purposes of analyzing, testing and screening the galactosidase inhibitor can be achieved conveniently and rapidly, and an effective means and a useful tool are provided for discovery and development of lead compounds and effective drugs capable of preventing and treating the galactosemia disease.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on galactokinase, carbamoyl phosphate synthetase, and isocitric acid dehydrogenation A series of catalyzed reactions of the enzyme is completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The present disclosure describes a genetically engineered bacteria that relieves the catabolite repression problem exerted by the Spot 42 small regulatory RNA by adding a galactokinase that does not contain the Spot 42 binding region. As such, galK and galM and the like can be expressed allow better galactose utilization.

By amplifying the upstream and downstream sequences of genome DNA of galactokinase of Rhodosporidium toruloides, a promoter and a terminator are obtained. The promoter and terminator can be widely applied to gene expression, operation of genetic engineering, and strain improvement of Rhodosoporidium, Sporidiobolus, Sporobolomyces, and Rhodotorula. The nucleotide sequences of the promoter and the terminator are SEQ ID No.1 and SEQ ID No.2 respectively. The invention also relates to a DNA expression cassette or recombinant carrier comprising the elements, a method utilizing related elements to establish gene engineering strains of Rhodosoporidium, Sporidiobolus, and Rhodotorula, and corresponding strains.