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Determination method of galactose and galactose diagnosis/measurement kit

A technology of galactose and galactokinase, applied in the field of medical/food inspection and determination, can solve the problems affecting the physical and intellectual development of infants and young children, and the absorption of elements and other problems

Inactive Publication Date: 2011-12-28
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Although milk is good, not all people can enjoy it. Due to the lack of lactase, the human body cannot digest and absorb the nutrients in milk well, and will Causes absorption disorders of calcium, phosphorus, potassium, iron and other elements, affecting the physical and intellectual development of infants and young children. In the elderly, lactase deficiency is an important cause of osteoporosis

Method used

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  • Determination method of galactose and galactose diagnosis/measurement kit
  • Determination method of galactose and galactose diagnosis/measurement kit
  • Determination method of galactose and galactose diagnosis/measurement kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0127] Example 1: Determination of galactose content in plasma

[0128] 1) Preparation of standard samples

[0129] Dissolve a certain amount of galactose in water or buffer, and then adjust the galactose concentration to 100 micromoles / liter;

[0130] 2) Preparation of samples to be tested

[0131] The plasma sample can be used directly as the sample to be tested;

[0132] 3) Blank sample

[0133] The water or buffer is used as a blank sample, and its galactose concentration is 0 micromol / L;

[0134] B. Preparation of reagent solution:

[0135] The galactose diagnostic / measurement reagent in this embodiment is a single-dose reagent, which contains:

[0136] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0137] Glycerin 1mol / L

[0138] NADPH 0.25mmol / L

[0139] Galactoskinase 16000U / L

[0140] Acetyl-CoA carboxylase 18000U / L

[0141] Formate dehydrogenase 12000U / L

[0142] Adenosine triphosphate 5mmol / L

[0143] Monovalent phosphate 15mmol / L

[0144] Maloyl-CoA 5mmol / L.

[0145...

Embodiment 2

[0157] Example 2: Determination of galactose content in plasma

[0158] 1) Preparation of standard samples

[0159] Dissolve a certain amount of galactose in water, and adjust the concentration of galactose to 100 micromol / L as a standard sample;

[0160] 2) Preparation of samples to be tested

[0161] The plasma sample can be used directly as the sample to be tested;

[0162] 3) Blank sample

[0163] The water is used as a blank sample, and its galactose concentration is 0 micromol / L;

[0164] B. Preparation of reagent solution:

[0165] The galactose diagnosis / determination reagent is a two-dose reagent, which contains:

[0166] Reagent 1 consisting of:

[0167] Phosphate buffer 100mmol / L

[0168] NADH / NAD + 0.25mmol / L

[0169] Adenosine triphosphate 5mmol / L

[0170] Maloyl-CoA 5mmol / L;

[0171] Reagent 2 consisting of:

[0172] Phosphate buffer 100mmol / L

[0173] Ethylene glycol 5mol / L

[0174] Galactoskinase 16000U / L

[0175] Acetyl-CoA carboxylase 32000U / L

[0176] Formate dehydro...

Embodiment 3

[0193] Example 3: Determination of galactose content in plasma samples

[0194] The operation mode of this embodiment is the same as that of embodiment 2, except that this embodiment:

[0195] B. Preparation of reagent solution:

[0196] The galactose diagnosis / determination reagent consists of three reagents, which contains:

[0197] Reagent 1 consisting of:

[0198] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0199] NADH 0.25mmol / L

[0200] Adenosine triphosphate 5mmol / L

[0201] Monovalent phosphate 15mmol / L

[0202] Maloyl-CoA 5mmol / L;

[0203] Reagent 2 consisting of:

[0204] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0205] Ammonium sulfate 1mol / L

[0206] Acetyl-CoA carboxylase 28000U / L

[0207] Formic acid dehydrogenase 50000U / L;

[0208] Reagent 3 consisting of:

[0209] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0210] Ammonium sulfate 2mol / L

[0211] Galactoskinase 26000U / L.

[0212] Add these reagents to deioniz...

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Abstract

The present invention relates to the measuring method of galactose content using enzyme colorimetric method and enzyme-linked method technology, the composition and component of reagent, the technical principle of its measuring is based on galactokinase, acetyl coenzyme A carboxylase, formate dehydrogenase The series of catalytic reactions are completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

Description

【Technical Field】 [0001] The present invention relates to the technical field of medical / food inspection and determination. More specifically, the present invention relates to a galactose diagnosis / measurement method and a kit thereof. 【Background technique】 [0002] Milk is known as "liquid gold" in the nutrition circle. As early as 1992, our country was jointly put forward by the seven parts: "A glass of milk strengthens a nation" slogan. In addition to not containing cellulose, milk contains almost all the nutrients needed by the human body. After the lactose in milk enters the human body, it is broken down into glucose and galactose by the action of small intestinal lactase. Galactose is an essential substance for infant brain development and is closely related to the rapid growth of infant brain. [0003] Although milk is good, not everyone can enjoy it. Due to the lack of lactase, the human body cannot digest and absorb the nutrients in milk very well. It can also cause the...

Claims

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Application Information

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IPC IPC(8): G01N33/66G01N21/31G01N35/00C12Q1/54C12Q1/48C12Q1/32C12Q1/25
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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