Fluorescent Probes for Analytical Detection and Screening of Galactokinase Inhibitors

Abstract

The invention discloses a fluorescent probe for analyzing, testing and screening a galactokinase inhibitor. The structure of the fluorescent probe is shown in the formula (I) in the specification, wherein X is O or NH, and R2 is selected from compounds as shown in the specification. The fluorescent probe can simplify the conventional screening process of the galactokinase inhibitor and is directly used in any cell system expressing galactokinase without use of purified and refined galactokinase protein; with the application of the fluorescent probe, purposes of analyzing, testing and screening the galactosidase inhibitor can be achieved conveniently and rapidly, and an effective means and a useful tool are provided for discovery and development of lead compounds and effective drugs capable of preventing and treating the galactosemia disease.

Description

technical field

[0001] The invention belongs to the field of biological analysis and detection, and in particular relates to a fluorescent probe for analysis, detection and screening of galactokinase inhibitors, a preparation method and an application.

[0002] technical background

[0003] Galactosemia is a disease in which galactose in the blood and urine increases. The disease that occurs frequently in newborns or infants is due to the genetic deficiency of galactose-l-phosphate uridyltransferase (GALT), which leads to galactose The first step metabolite of lactose, galactose-l-phosphate (Gal-1-P), is caused by a large accumulation of tissues and cells in the patient's body. The main symptoms of galactosemia are nutritional disorders, cataracts, jaundice, diarrhea, sepsis, mental retardation and hepatosplenomegaly, neonatal death, etc. The clinical treatment of galactosemia mainly includes intravenous glucose supplementation therapy, fresh plasma infusion, electrolyte sup...

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