Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for enhancing production of alpha-linolenic acid from rhodosporidium toruloides

A technology of Rhodosporidium toruloides and linolenic acid, which is applied in the field of strengthening the production of α-linolenic acid by Rhodosporidium toruloides, can solve the problems of low production of α-linolenic acid, achieve easy process control, increase utilization value, and simple operation Effect

Active Publication Date: 2016-03-02
江苏东誉绿塑生物科技有限公司
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In order to solve the problem of low yield of α-linolenic acid produced by Rhodosporidium toruloides, the present invention provides a method for strengthening the production of α-linolenic acid by Rhodosporidium toruloides, which effectively improves the production of α-linolenic acid by Rhodosporidium toruloides. - Yield of linolenic acid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Rhodosporidium toruloides ACCC20341 was used as the fermentation strain.

[0038] Rhodosporidium toruloides strain R.toruloides was activated from the slant preservation medium, and the composition of the slant preservation medium was YEPD: glucose 20g / L, yeast powder 10g / L, peptone 10g / L, agar powder 15g / L, pH5.8~ 6.0, sterilized by saturated steam at 115°C for 20min. The Rhodosporidium toruloides strain preserved above was activated using YEPD medium, cultured in a 500mL Erlenmeyer flask with a liquid volume of 200mL, 30°C, 200rpm for 48h to OD 600 =12.

[0039] Then the yeast cells were centrifuged to collect the thallus, and inoculated into a 10L fermenter for fed-batch fermentation culture, with a liquid volume of 6L. The ingredients of fed-batch fermentation medium are: glucose 20g / L, xylose 30g / L, sodium glutamate 6.2g / L, inorganic salt KH 2 PO 4 :0.52%, K 2 HPO 4 :0.16%, MgCl 2 :0.48%. During the fermentation process, by feeding a mixed carbon source aqu...

Embodiment 2

[0041] The strain Rhodosporidium toruloides R. toruloides M18, which can grow and metabolize in non-detoxified cellulose hydrolyzate obtained by mutagenesis of Rhodosporidium toruloides ACCC20341 at normal temperature and pressure (Atmospheric and room temperature plasma) (references: FengQi, Yuki Kitahara, Zitian Wang, Xuebing Zhao, WeiDu, Dehua Li. Novel mutant strains of Rhodosporidium toruloides by plasmamutagenesis approach and their tolerance for inhibitors in lignocellulosic hydrolyzate. Journal of Chemical Technology and Biotechnology. 2014, 89(5): 735–742).

[0042] Use YEPD medium to activate the above-mentioned mutagenic R.toruloidesM18, culture in a 500mL Erlenmeyer flask with a liquid volume of 200mL, culture at 30°C, 200rpm for 48h to OD 600 =12. Then the yeast cells were centrifuged to collect the thallus, and inoculated into a 10L fermenter for fed-batch fermentation culture, with a liquid volume of 6L.

[0043] Use cellulose hydrolyzate as the main component ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for enhancing production of alpha-linolenic acid from rhodosporidium toruloides. The method comprises the following steps: 1) culturing activated rhodosporidium toruloides in a YEPD culture medium; and 2) collecting a rhodosporidium toruloides strain cultured in the step 1), inoculating the strain to a fed-batch fermentation culture medium and conducting fed-batch fermentation, wherein the fed-batch fermentation culture medium consists of a carbon source, a nitrogen source and inorganic salt; preferably, the carbon source is a mixed carbon sucrose containing glucose and xylitol; and during fed-batch fermentation process, a total carbon-to-nitrogen ratio is constantly kept at 100 to (0.7-1.5). The method disclosed by the invention is simple to operate and easy to control; and the yield of alpha-linolenic acid can reach more than 5% of total lipid.

Description

technical field [0001] The invention relates to a method for producing α-linolenic acid by using microorganisms, especially a method for strengthening the production of α-linolenic acid by Rhodosporidium toruloides. Background technique [0002] Microbial oil, also known as single-cell oil (SCO, single cell oil), is produced by microorganisms such as yeast, mold, bacteria, and algae under certain conditions, using carbohydrates, hydrocarbons, and ordinary oils as carbon sources. a lot of fat. Compared with the traditional oil production process, the use of microorganisms to produce oil has many special advantages in addition to high oil content: such as rapid microbial cell proliferation and short production cycle; the use of microorganisms to produce oil requires less labor than agricultural oil production. less, and not limited by site facilities, seasonal factors and weather changes; the biomass raw materials required for microbial growth and metabolism have a wide range...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P7/64C12R1/645
Inventor 黄建忠祁峰张明亮张媛江贤章
Owner 江苏东誉绿塑生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com