Application of microbial preparation for efficiently converting heavy metal cadmium in polluted soil

A technology of microbial preparations and polluted soil, applied in the application field of microbial bacterial preparations, can solve the problems of high economic cost, long-term survival, poor universality, etc., and achieve the effect of broad application prospects, simple and easy to control operation, and strong acid production capacity

Active Publication Date: 2017-12-01
HUNAN AGRI BIOTECH RES CENT +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] Among the relevant patents that have been applied for in China, the patent "A Method for Microbial Remediation of Heavy Metal Contaminated Soil" (application number: 201110122739.6, publication date: 2011‐11‐16) discloses a method of treating soil with Rhodobacter sphaeroides preparations combined with flooding The method, whose working principle is mainly to use Rhodobacter sphaericus to absorb heavy metals after multiplying in a large amount in a water-rich anaerobic environment, and then infiltrate into the ground through water to reduce the concentration of heavy metals in the soil, but the soil cannot be used for planting operations during the treatment process. The economic cost is high. On the other hand, the strains are too single, the universality is poor, the scope of application is small, and the types and degrees of heavy metals that can be tr

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: collection, cultivation and domestication of cadmium-tolerant microorganisms

[0027] Group 1 consists of 71.5% Candida, 13.5% Phialemonium, 10% Rhodosporidium toruloides, 5% Cryptococcus; Candida (Candida) includes Candida parapsilosis (Candida parapsilosis), Tropical Candida (Candidatropicalis) and stout Candida (Candida valida), the number ratio of the three bacteria is 5:2:3; Cryptococcus (Cryptococcus) includes The ratio of Cryptococcus laurentii, Cryptococcus victoriae and Filobasidiella bacillispora is 3:2:2;

[0028] Before mixing, the bacteria were respectively acclimatized in cadmium-containing medium. The specific steps and conditions of acclimation were as follows: put the bacteria in 100mL sterilized medium containing 10% 9K, 8‰ glucose, 4‰ sulfur powder and a concentration gradient of 0.5mmol / L, 1.0mmol / L, 1.5mmol / L, 2.0mmol / L, ..., 14.5mmol / L, 15.0mmol / L CdSO 4 In the culture medium, acclimate 30 generations under the condition of rotatio...

Embodiment 2

[0033] Embodiment 2: Aseptic soil removes cadmium experiment

[0034] The soil was also collected from contaminated farmland soil in the VIP experimental demonstration area of ​​environmental governance in Beishan Town, Hunan Province, numbered 1, 2, and 3. The soil was air-dried, ground and passed through a 100-mesh sieve, and then packaged and sterilized.

[0035] Experimental design: Dilute the bacterial concentration of the microbial agent to 4.0×10 with the culture medium 7 pcs / mL—7.0×10 7 Each / mL, put into this microbial inoculum agent according to the ratio of cadmium-contaminated soil quality and diluted bacterial liquid volume 0.5kg:1L as the experimental group, and each sample is set with 3 parallel experimental groups and 1 blank control group (adding the same ratio Cadmium-contaminated soil, no bacterial agent), the initial pH is 2.75, cultivated at 30°C and 150rpm. Measure the pH and Eh every day, centrifuge to collect the slag after 4.5 days of leaching, air-d...

Embodiment 3

[0037] In this embodiment, the field effect verification is carried out in the experimental soil. The situation is as follows. Take the polluted farmland soil in the VIP experimental demonstration area of ​​environmental governance in Beishan Town, Changsha City, Hunan Province, and measure the Cd content in the soil for 3 times. The average value is 2.31ppm. The specific steps of applying the microbial preparation of the present invention are as follows: ① uniformly apply the microbial preparation on the polluted soil surface area according to the ratio of 40L / mu-60L / mu. ②Use an agricultural tiller to plow the ground for 1 hour, settle overnight and then drain to the recovery pond to measure the cadmium content in the soil. ③ Repeat steps ① and ② once every 1-2 days, and the microbial preparations can be recycled, that is, the cadmium in the solution has been recycled to treat the microbial preparations. Repeating this treatment three times can restore the soil polluted by he...

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Abstract

The invention discloses an application of a microbial preparation for efficiently converting heavy metal cadmium in polluted soil. The microbial preparation is prepared by performing enriched cultivation on the following two mixed floras: (1) candida mycoderma bacteria, aspergillus monicus, rhodosporidium toruloides and cryptococcus which form a flora 1; and (2) acidithiobacillus thiooxidans, leptospirillum ferrooxidans, acidithiobacillus caldus, sulfobacillus thermosulfidooxidans, acidithiobacillus ferrooxidans and thermos ferric bacteria which form a flora 2. By using the microbial preparation to treat the cadmium polluted soil, the metal cadmium is converted from being indissolvable into being soluble and the removal rate is 70-82%. The microbial preparation disclosed by the invention has the advantages of being high in conversion efficiency, short in period, low in cost, free of secondary pollution, easy to popularize and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment of heavy metal pollution, and in particular relates to the application of a microbial bacterial agent capable of efficiently biologically transforming the form of cadmium in polluted soil. Background technique [0002] In recent years, my country has achieved world-renowned achievements in economic development. However, excessive industrial activities have brought serious environmental pollution, posed a serious threat to the health of residents, and brought huge hidden dangers to the sustainable development of society, economy and environment. . my country's rural environment is facing a variety of environmental problems, mainly including soil heavy metal pollution, soil fertility decline, cultivated land salinization, river water eutrophication, and environmental pollution caused by the abuse of pesticides, fertilizers, and insecticides. [0003] According to relevant national data, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14B09C1/10C12R1/72C12R1/645C12R1/01
CPCB09C1/105C12N1/14C12N1/16C12N1/20
Inventor 蒋慧丹柏连阳刘学端刘宏伟梁伊丽尹华群刘亚姿
Owner HUNAN AGRI BIOTECH RES CENT
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