Microbial preparation efficiently converting heavy metal cadmium in contaminated soil and preparation method thereof
A technology for microbial preparation and polluted soil, which is applied in the field of high-efficiency biotransformation of microbial inoculants in the form of cadmium in polluted soil and their preparation, can solve the problems of high economic cost, long-term survival, poor universality and the like, and achieves broad application prospects and operation. Simple and easy to control, the effect of strong acid production ability
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Embodiment 1
[0026] Embodiment 1: collection, cultivation and domestication of cadmium-tolerant microorganisms
[0027] Group 1 consists of 71.5% Candida, 13.5% Phialemonium, 10% Rhodosporidium toruloides, 5% Cryptococcus; Candida (Candida) includes Candida parapsilosis (Candida parapsilosis), Tropical Candida (Candidatropicalis) and stout Candida (Candida valida), the number ratio of the three bacteria is 5:2:3; Cryptococcus (Cryptococcus) includes The ratio of Cryptococcus laurentii, Cryptococcus victoriae and Filobasidiella bacillispora is 3:2:2;
[0028] Before mixing, the bacteria were respectively acclimatized in cadmium-containing medium. The specific steps and conditions of acclimation were as follows: put the bacteria in 100mL sterilized medium containing 10% 9K, 8‰ glucose, 4‰ sulfur powder and a concentration gradient of 0.5mmol / L, 1.0mmol / L, 1.5mmol / L, 2.0mmol / L, ..., 14.5mmol / L, 15.0mmol / L CdSO 4 In the culture medium, acclimate 30 generations under the condition of rotatio...
Embodiment 2
[0033] Embodiment 2: Aseptic soil removes cadmium experiment
[0034] The soil was also collected from contaminated farmland soil in the VIP experimental demonstration area of environmental governance in Beishan Town, Hunan Province, numbered 1, 2, and 3. The soil was air-dried, ground and passed through a 100-mesh sieve, and then packaged and sterilized.
[0035] Experimental design: Dilute the bacterial concentration of the microbial agent to 4.0×10 with the culture medium 7 pcs / mL—7.0×10 7 Each / mL, put into this microbial inoculum agent according to the ratio of cadmium-contaminated soil quality and diluted bacterial liquid volume 0.5kg:1L as the experimental group, and each sample is set with 3 parallel experimental groups and 1 blank control group (adding the same ratio Cadmium-contaminated soil, no bacterial agent), the initial pH is 2.75, cultivated at 30°C and 150rpm. Measure the pH and Eh every day, centrifuge to collect the slag after 4.5 days of leaching, air-d...
Embodiment 3
[0037] In this embodiment, the field effect verification is carried out in the experimental soil. The situation is as follows. Take the polluted farmland soil in the VIP experimental demonstration area of environmental governance in Shanzhen Town, Changsha City, Hunan Province, and measure the Cd content in the soil for 3 times. The average value is 2.31ppm. The specific steps of applying the microbial preparation of the present invention are as follows: ① uniformly apply the microbial preparation on the polluted soil surface area according to the ratio of 40L / mu-60L / mu. ②Use an agricultural tiller to plow the ground for 1 hour, settle overnight and then drain to the recovery pond to measure the cadmium content in the soil. ③ Repeat steps ① and ② once every 1-2 days, and the microbial preparations can be recycled, that is, the cadmium in the solution has been recycled to treat the microbial preparations. Repeating this treatment three times can restore the soil polluted by h...
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