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Growth-promoting bacterial strain for promoting rooting of cutting tea seedlings and microbial seedling culturing substrate thereof

A seedling-raising substrate and microorganism technology, applied in the direction of microorganism-based methods, chemicals for biological control, microorganisms, etc., can solve the problems of being easily restricted by natural conditions, occupying land for a long time, and low reproductive survival rate, so as to promote Rapid callus and growth, reduced seedling cycle, and high seedling efficiency

Inactive Publication Date: 2017-08-29
TEA RES INST ANHUI ACAD OF AGRI SCI
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Problems solved by technology

However, the above-mentioned asexual reproduction techniques generally have disadvantages such as long seedling cycle, low reproductive survival rate, and easy to be restricted by natural conditions.
The practical results show that it takes 70-90 days for spring and summer tea seedlings from March to August to root, and more than 90 days for autumn tea seedlings from October to November to root; the whole seedling cultivation cycle reaches 16-90 days. 18 months, occupying land for a long time and resulting in high cultivation costs

Method used

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  • Growth-promoting bacterial strain for promoting rooting of cutting tea seedlings and microbial seedling culturing substrate thereof
  • Growth-promoting bacterial strain for promoting rooting of cutting tea seedlings and microbial seedling culturing substrate thereof
  • Growth-promoting bacterial strain for promoting rooting of cutting tea seedlings and microbial seedling culturing substrate thereof

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Embodiment Construction

[0024] For ease of understanding, here the technical scheme of the present invention is further described as follows:

[0025] 1. Obtaining strain WYS-2:

[0026] The invention provides a growth-promoting bacterial strain for promoting rooting of cutting tea seedlings, that is, Rhodosporidium toruloides WYS-2 strain. The process of obtaining the WYS-2 strain is to collect the tea tree rhizosphere soil growing vigorously in the tea garden for more than 30 years, and adopt the selective medium of anodic acid bacteria, nitrogen-free culture method, inorganic phosphorus medium and auxin assay (IAA) A four-part screening method was used to obtain aluminum-resistant growth-promoting strains. Then through the tomato seed germination test and the tea seedling seedling tray test, the high-efficiency growth-promoting strain WYS-2 was finally obtained, and the strain was stored in a 4°C refrigerator.

[0027] Strain WYS-2 belongs to Rhodosporidium toruloides, such as figure 1 As shown...

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Abstract

The invention belongs to the technical field of tea cultivation and particularly relates to a growth-promoting bacterial strain for promoting the rooting of cutting tea seedlings and a microbial seedling culturing substrate thereof. The growth-promoting bacterial strain is bacterial strain WYS-2 belonging to Rhodosporidium toruloides and preserved in CGMCC (China General Microbiological Culture Collection Center) with the culture preservation number of CGMCC NO.13524 on January 3rd 2017. Above 1*108cfu / g of colony-forming unit of the bacterial strain WYS-2 is contained in the microbial seedling culturing substrate. Alumite and the tea root growth-promoting bacterial strain WYS-2 are firstly obtained and the microbial seedling culturing substrate is prepared later. By means of the microbial seedling culturing substrate, the rooting time of cutting tea seedlings is obviously shortened; the callus rate and the rooting percentage of the cutting tea seedlings are obviously increased; the tea seedling culturing period is shorter; and the seedling culturing efficiency is higher.

Description

technical field [0001] The invention belongs to the technical field of tea cultivation, and in particular relates to a growth-promoting bacterial strain for promoting rooting of cutting tea seedlings and a microbial seedling-raising substrate thereof. Background technique [0002] Tea tree is one of the main economic crops in my country. There are many varieties of tea trees. In order to maintain the characteristics and characteristics of fine varieties, most of them use short ear cuttings and other asexual propagation techniques for tea tree seedling cultivation. However, the above-mentioned asexual reproduction techniques generally have disadvantages such as long seedling raising period, low reproductive survival rate, and being easily restricted by natural conditions. The practical results show that it takes 70-90 days for spring and summer tea seedlings from March to August to root, and more than 90 days for autumn tea seedlings from October to November to root; the who...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A01N63/04A01P21/00C05G3/00A01G9/10C12R1/645
CPCA01G9/029A01N63/30C05D9/00C05G3/00C12N1/16C12N1/145C12R2001/645C05F11/02C05F11/08
Inventor 罗毅方吴云赵强王烨军刘丹丹丁勇
Owner TEA RES INST ANHUI ACAD OF AGRI SCI
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