Plant defense signal peptides

a plant defense and signal peptide technology, applied in the field of plant defense signal peptides, can solve the problems of many interactions that are harmful to plants, damage to plants, and decimation of ornamental plants, etc., and achieve the effects of reducing disease symptoms, enhancing plant defenses, and improving plant product yield

Inactive Publication Date: 2009-12-03
WASHINGTON STATE UNIVERSITY
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Benefits of technology

[0028]According to another embodiment, an isolated polynucleotide is provided that encodes a polypeptide comprising a 10 amino acid peptide motif, where the 10 amino acid peptide motif consists of a glycine residue at position C-7 of the peptide, and the polypeptide causes a change of at least 0.2 pH units at a concentration of 25 pM / ml in a plant cell suspension, where the isolated polynucleotide further comprises a promoter, where the expression of the isolated polynucleotide in a cell of a plant causes the plant to exhibit an improvement compared to a control plant lacking the polynucleotide. The improvement may be an improved yield of plant product, reduced disease symptoms, or enhanced resistance to disease infestation.
[0050]According to another embodiment, methods are provided for conferring on a plant cell a response to a plant defense signal peptide to which the plant cell would not normally respond, the method comprising expressing in the plant cell a polynucleotide comprising a sequence encoding a receptor for the plant defense signal peptide (including, for example, AtPepR1 or AtPepR2) operably linked to a promoter that is expressible in the plant cell. The plant cell response could include, for example, alkalinization of the plant cell in response to administration of the plant defense signal peptide, transcription of pathogen defense genes, enhanced resistance to a pathogen, etc.

Problems solved by technology

Although many of the interactions between these organisms and plants, particularly via the roots of the plants, are beneficial, many of the interactions are harmful to the plants.
The decimation of agricultural crops, ornamental plants, and other plants by diseases caused by plant pathogens is a worldwide problem that has enormous economic impact.
Damage to plants is caused by pathogens of multiple genera.
The activity of these compounds is typically limited to several species.
As a consequence of the large number and diversity of plant pathogens, these compounds have not provided an effective solution to limiting infections in plants.
However, resistance may be limited to certain genera of pathogens, or crops of agronomic interest may not exhibit sufficient resistance.
Thus, natural plant defenses often do not provide sufficient protection against pathogens.

Method used

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  • Plant defense signal peptides
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Examples

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example 1

Isolation and Analysis of AtPep1 and Paralogs and Orthologs Thereof

[0214]Innate immunity is initiated in animals and plants through the recognition of a variety of pathogen associated molecules that in animals are called “pathogen-associated molecular patterns,” or PAMPS, and in plants are called elicitors. Peptides derived from pathogens can be powerful elicitors of plant defense responses (Hahlbrock et al., Proc. Natl. Acad. Sci. USA 92:4150-4157, 1995; van den Askerveken et al., Plant Physiol. 103:91-96, 1993; Kammpren, Curr. Opin. Plant Biol. 4:295-300, 2001; Kunze et al., Plant Cell, 16:3496-3507, 2004; Navarro et al., Plant Physiol. 135:1113-1128, 2004; Fellbrich et al., Plant J. 32:375-390, 2002; He et al., Cell 73:1255-1266, 1993), but plant-derived peptides have not been identified previously that are elicitors of immune responses directed against pathogens.

[0215]We have isolated and characterized a 23 amino acid peptide, called AtPep1, that is a signaling component of the ...

example 2

An LRR Receptor Kinase is a Component of AtPep1 Amplification of Innate Immunity in Arabidopsis

[0251]Over 200 LRR receptor kinases are present in the Arabidopsis genome. Only a few LRR receptor kinases have been identified that interact with the peptide signals in plants. This includes the receptor for the defense peptide signal, systemin (Scheer and Ryan, Proc. Natl. Acad. Sci. 99:9585-9590, 2002) and receptors for the developmental peptide signals CLAVATA1 (Clark and Meyerowitz, Cell 89:575-585, 1997), phytosulfokine-alpha (Matsubayashi et al., Science 296:1470-1472, 2002) and the pathogen-derived peptide flg22 (Gomez-Gomez and Boller, Trends Plant Sci. 7:251-256, 2000).

[0252]The discovery that the AtPep family of endogenous peptides from Arabidopsis leaf extracts cause an alkalinization of the medium of Arabidopsis suspension cultured cells indicates that the peptide plays a role in plant cells by interacting with a cell surface receptor. Investigations of the biological role of...

example 3

Shorter Peptides from the C-Terminus of AtPep1 Possess Substantial Defense Signal Peptide Activity

[0268]Analogs of AtPep1 from the C-terminus of AtPep1 were synthesized and assayed in the alkalinization assay. One mL aliquots of 4 day old Arabidopsis cells were allowed to equilibrate on an orbital shaker at 180 rpm for one hour. A 10 μL aliquot of each peptide solution was added to the cells. Peptide concentrations of 0.25 nM, 2.5 nM, and 25 nM were tested. After 20 min, the pH of the media was recorded. The results are shown in FIG. 5. An analog of AtPep1 missing the carboxy-terminal amino acid was completely inactive, whereas deletions from the amino-terminus of the peptide resulted in a sequential reduction in activity, until peptides with 9 carboxy-terminal amino acids remaining (SSGRPGQHN (SEQ ID NO: 75)) were inactive. A peptide with 10 carboxy-terminal amino acids remaining had substantial defense signal peptide activity at the 25 nM peptide concentration, causing a change in...

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Abstract

A 23 amino acid peptide, AtPep1, plays an important role as a signaling component of the innate immune system of Arabidopsis. The peptide precursor gene is transcribed in response to elicitors generated by pathogens, and AtPep1 is produced to amplify the signaling pathways. Seven paralogs of the AtproPep1 gene have been identified in the Arabidopsis genome, and orthologs have been identified in species of several agriculturally important families. AtPep1 and its paralogs and orthologs play important roles as endogenous signals to amplify innate immunity. The sequences of two AtPep1 receptors from Arabidopsis are also provided.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a Continuation-in-Part of U.S. patent application Ser. No. 11 / 795,733, filed Jun. 4, 2008, which is a national stage entry of PCT / US2006 / 002661, filed Jan. 24, 2006, which claims priority to U.S. Provisional Patent Application No. 60 / 647,708, filed Jan. 26, 2005, this application also claims the benefit of U.S. Provisional Patent Application No. 61 / 124,199, filed Apr. 14, 2008, all of which are hereby incorporated by reference in their entirety.GOVERNMENT LICENSE RIGHTS STATEMENT[0002]The U.S. Government has a paid-up license in this invention and the right in limited circumstances to require the patent owner to license others on reasonable terms as provided for by the terms of Grant No. IBN 0090766 awarded by the National Science Foundation.FIELD[0003]The present disclosure relates to materials and methods for enhancing plant disease resistance.BACKGROUND[0004]Plants are exposed to numerous denizens of their environme...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01H5/00C12N15/11C07K7/00C07K14/415A01N33/00
CPCC07K14/415C12N15/8282C12N15/8279
Inventor RYAN, CLARENCE A.RYAN, PATRICIA LOUISEPEARCE, GREGORY L.HUFFAKER, ALISAYAMAGUCHI, YUBE
Owner WASHINGTON STATE UNIVERSITY
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