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Application of burdock fructooligosaccharides as disease-resistant elicitors in agriculture

A technology of burdock fructooligosaccharides and elicitors, applied in the field of functional oligosaccharides, to achieve the effect of improving persistence and stability, good development value, and good market prospects

Inactive Publication Date: 2007-08-01
SHANDONG YINGTIAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of burdock fructooligosaccharides as disease-resistant elicitors in agriculture

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1: the growth-promoting effect of burdock fructo-oligosaccharides on plants

[0022] The tested cucumber (Cucumis sativus L.) seeds were "Lu 3" disease-resistant variety.

[0023] Burdock oligosaccharide (Burdock oligosaccharide, BOS), Mr>2000, DP13, oligosaccharide content 90%. Prepare 1.0, 3.0, 5.0, 8.0, 10.0 g / L solutions (hereinafter referred to as BOS solutions) respectively with distilled water for future use.

[0024] Determination of growth indicators:

[0025] Root length refers to the distance between the lowest point of the root of the plant and the soil surface; plant height refers to the length between the soil surface and the highest growth point; stem thickness refers to the thickness at 2 cm below the cotyledon; the underground part of the seedling (including root system and underground stem) , The dry weight of the aboveground part is determined by 80 ℃ drying method;

[0026] (1) The effect of burdock fructo-oligosaccharide treatment on...

Embodiment 2

[0034] Embodiment 2: Pot experiment burdock fructo-oligosaccharides induces effect on resistance to cucumber powdery mildew

[0035] The tested cucumber (Cucumis sativus L.) seeds were "Lu 3" disease-resistant variety. Cucumber seeds were sterilized with 5% NaClO solution for 15 minutes, then rinsed with clear water, placed in an incubator at 27°C to accelerate germination, selected dew-white seeds were sown in sterile nutrient soil, and seedlings were grown in a solar greenhouse (temperature and humidity were the same as in Example 1). , for routine management. When the cucumber seedlings are at the 3-leaf stage, set aside.

[0036] Burdock oligosaccharide (Burdock oligosaccharide, BOS), Mr>2000, DP13, oligosaccharide content 90%.

[0037] Prepare a 5.0 g / L solution with distilled water for later use.

[0038] Cucumber powdery mildew (Sphaerotheca cucurbitca) originated from the diseased cucumber leaves grown in greenhouses. After the diseased leaves are harvested, keep t...

Embodiment 3

[0049] Embodiment 3: Pot experiment burdock fructo-oligosaccharides induces effect on cucumber resistance to Fusarium wilt

[0050] The cucumber (Cucumis sativus L.) seeds tested were "Lu 10" disease-resistant variety. Cucumber seeds were sterilized with 3% sodium hypochlorite, rinsed, imbibed, and germinated, then planted in sterilized nutrient soil, and raised seedlings in a solar greenhouse (with the same temperature and humidity as in Example 1) for routine management. When the cucumber grows to the 4-leaf stage, set it aside.

[0051] Burdock fructooligosaccharide, same as embodiment 2.

[0052] Cucumber Fusarium oxysporum (Fusarium oxysporum) originated from the diseased cucumber leaves grown in greenhouses. After the strain was cultured on PDA medium at 22°C for 7 days, the spore suspension was prepared with sterile water at a concentration of 10 7 spores / ml.

[0053] Spray burdock fructo-oligosaccharides on the cucumber leaves of the test plants, and inoculate afte...

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Abstract

The invention discloses the application of burdock fructo-oligosaccharide as disease resistance elicitor in agriculture. Burdock fructo-oligosaccharide is natural oligosaccharide with good water solubility and stability extracted form burdock root. The invention comprises applying burdock fructo-oligosaccharide as disease resistance elicitor to promote plant growth and induce plant disease resistance. Burdock fructo-oligosaccharide can promote plant growth in different seedling stages, reinforce procatarxis of the seedlings, and promote the formation of strong seedlings.

Description

(1) Technical field [0001] The invention relates to a functional oligosaccharide with the role of a disease resistance elicitor in agriculture, in particular to the application of burdock fructooligosaccharide as a disease resistance elicitor. (2) Background technology [0002] In recent years, my country's facility agriculture has developed rapidly, and the degree of intensification has increased, and continuous planting has caused serious plant diseases. Plant diseases have a great impact on agricultural production. When the disease breaks out on a large scale, it will cause serious production reduction and directly affect the quality of plants. On the one hand, the main diseases of facility vegetables are serious. In order to control the outbreak and prevalence of diseases and reduce the huge losses caused by diseases, they basically rely on the use of chemically synthesized fungicides. Excessive use of chemically synthesized pesticides reduces the quality of agricultural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N35/02A01P21/00A01P1/00
Inventor 陈靠山张宗席
Owner SHANDONG YINGTIAN BIO TECH
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