Ganoderma lucidum mycelium liquid fermentation method improving ganoderic acid content with fungal elicitor

A technology of fungal elicitor and ganoderma mycelium, which is applied in the field of bioengineering to achieve the effect of increasing content, simple operation process, and good industrial application prospects

Inactive Publication Date: 2009-01-28
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the use of fungal elicitors for edible and medicinal fungal cell culture. At present, there are only repo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Strain: the fungal elicitor strain is Acremonium strictum, purchased from China General Microorganism Culture Collection Center, and the strain number is CGMCCNo.3.2058.

[0026] Ganoderma lucidum is a common cultivar (Ganoderma lucidum), purchased from China General Microorganism Culture Collection Center, numbered CGMCC No.5.644.

[0027] A method for liquid fermentation of ganoderma mycelium using fungal elicitors to increase ganoderma acid content, characterized in that it comprises the following steps:

[0028] (1) Preparation of fungal elicitor:

[0029] ①Cultivation of fungal elicitor strains: Inoculate fungal elicitor strains on PDA slant medium for activation, then transfer to PDA plate medium, culture at 25°C for 5 days, and use a puncher to punch out a diameter of 0.3 at the edge of the colony. Inoculate 3 pieces of bacterial blocks of cm in PDA liquid medium, fill each 500mL Erlenmeyer bottle with 100mL of PDA liquid medium, cultivate it in a shaker at 25°C...

Embodiment 2

[0039] Strain: The fungal elicitor strain is Trichodermaviride, a fungus of the genus Trichoderma, purchased from China General Microorganism Culture Collection Center, and the strain number is CGMCCNo.3.2196.

[0040]Ganoderma lucidum is a common cultivar (Ganoderma lucidum), which was purchased from China General Microorganism Culture Collection Center, numbered CGMCC No.5.644.

[0041] A method for liquid fermentation of ganoderma mycelium using fungal elicitors to increase ganoderma acid content, characterized in that it comprises the following steps:

[0042] (1) Preparation of fungal elicitor:

[0043] ①Cultivation of fungal elicitor strains: Inoculate fungal elicitor strains on PDA slant medium for activation, then transfer to PDA plate medium, culture at 30°C for 3 days, and use a puncher to punch out a diameter of 0.6 at the edge of the colony. Inoculate 5 pieces of bacterial blocks of cm in PDA liquid medium, fill each 500mL Erlenmeyer bottle with 200mL of PDA liqui...

Embodiment 3

[0053] Strain: the fungal elicitor strain is Verticillium psalliotae, purchased from China General Microorganism Culture Collection Center, and the strain number is CGMCC No.3.4423.

[0054] Ganoderma lucidum is a common cultivar (Ganoderma lucidum), which was purchased from China General Microorganism Culture Collection Center, numbered CGMCC No.5.644.

[0055] A method for liquid fermentation of ganoderma mycelium using fungal elicitors to increase ganoderma acid content, characterized in that it comprises the following steps:

[0056] (1) Preparation of fungal elicitor:

[0057] ①Cultivation of fungal elicitor strains: Inoculate fungal elicitor strains on PDA slant medium for activation, then transfer to PDA plate medium, culture at 28°C for 4 days, and use a puncher to punch out a diameter of 0.4 at the edge of the colony. Inoculate 4 pieces of bacterial blocks of cm in PDA liquid medium, fill each 500mL Erlenmeyer bottle with 150mL of PDA liquid medium, culture in a shak...

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PUM

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Abstract

The invention relates to a Ganoderma lucidum mycelium liquid fermentation method for improving ganoderic acid content by utilizing Fungal Elicitors. The method comprises the steps of the preparation of the Fungal Elicitors, the liquid fermentation culture of the Ganoderma lucidum and the co-culture of the Ganoderma lucidum and the Fungal Elicitors. The method has the advantages of simple process, low cost, obvious effect, high content of the ganoderic acid, and being a manufacturing method with huge industrial prospect.

Description

Technical field: [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a liquid fermentation method for producing high-yield ganoderma acid by using fungal elicitors. Background technique: [0002] Ganoderma lucidum belongs to Basidiomycetes, Polyporaceae, Ganoderma fungus. Modern pharmacological studies have shown that Ganoderma lucidum has anti-tumor, anti-aging, and anti-arteriosclerosis effects, and has high pharmacological activity against cardiovascular and cerebrovascular diseases. The polysaccharides, triterpenoids (mainly ganoderma acid), alkaloids, amino acid polypeptides, trace elements and other components in Ganoderma lucidum are the basis of medicinal substances of Ganoderma lucidum. Among them, ganoderma acid is the material basis for ganoderma to treat hypertension, hyperlipidemia, insomnia and various respiratory diseases (such as removing phlegm, relieving cough, and relieving asthma) and anti-tumor. While the...

Claims

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Application Information

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IPC IPC(8): C12P7/40C12R1/645
Inventor 高兴喜刘林德王玥张秋胜杨润亚韩凯
Owner LUDONG UNIVERSITY
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