Fermentation method for high cordyceps militaris biomass and high cordycepin content

A high cordycepin and fermentation method technology, applied in the field of bioengineering, can solve the problems of high labor intensity, low production efficiency, and large land occupation, and achieve the effects of increasing the content of active substances, reducing production costs, and improving product quality

Inactive Publication Date: 2009-10-14
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0003] At present, the production of Cordyceps militaris is still dominated by solid cultivation, because of its long cycle, high labor intensity, large land occupation, l

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  • Fermentation method for high cordyceps militaris biomass and high cordycepin content

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Embodiment Construction

[0028] 1. Elicitor optimization:

[0029] The results of the preliminary experiment showed that molasses, corn steep liquor, and calcium chloride in the medium had a significant impact on the biomass of Cordyceps militaris. Effect of leather content.

[0030] In laboratory shaker culture experiments, Cordyceps strains were mixed with improved PDA liquid (potatoes 200g, glucose 20g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 1g, vitamin B 1 0.01g, water 1000ml) for 7 days to reach the maximum biomass of 8.69g / L, the content of cordycepin in mycelium is 0.47mg / g;

[0031] The composition of Cordyceps militaris fermentation medium optimized with agricultural and sideline products as raw materials is: molasses 20g / L, corn steep liquor dry powder 10g / L, calcium chloride 0.25g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, water 1000mL, in this substratum, the biomass that Cordyceps militaris cultivates for 7 days is 11.62g / L, and the...

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Abstract

The invention provides a fermentation method for high cordyceps militaris biomass and high cordycepin content, which belongs to the technical field of bio-engineering. A cordyceps militaris strain is activated, inoculated to a first stage seed tank and further expanded to a fermentation tank, when a certain biomass is achieved in the fermentation tank, a biotic elicitor of Xylaria type endophytic fungus from binkgo biloba extract liquid and non-biotic elicitors of sodium acetate and ammonium sulfate are added for promoting the production of cordycepin in bacteria. The fermentation method adopts an optimized culture medium for effectively increasing the mycelial growth of cordyceps militaris; furthermore, under the regulation role of the elicitors, the cordyceps militaris biomass and the cordycepin content in the bacteria are respectively increased by 1.76 times and 7.14 times compared with the contrast culture medium without the addition of the elicitors and respectively increased by 2.35 times and 23.25 times compared with the ordinary improved PDA. The fermentation method greatly improves the production amount of cordyceps militaris mycelia and the content of the active characteristic ingredient, thereby having broad market prospect.

Description

1. Technical field [0001] The invention is a fermentation method for high biomass of Cordyceps militaris and high content of cordycepin, which applies modern biological control technology to deep liquid fermentation of Cordyceps militaris, is specially used for the production of mycelium of Cordyceps militaris rich in cordycepin, and belongs to biological engineering technology field. 2. Background technology [0002] Cordyceps militaris, also known as Cordyceps militaris in my country, is a Cordyceps fungus that can parasitize pupae and larvae of various Lepidoptera insects. Cordyceps militaris and Cordyceps sinensis are very similar in medicinal chemistry and pharmacology, and have good practical value in functional food, medicine and biotechnology. In recent years, some drugs and functional foods called Cordyceps sinensis produced and marketed at home and abroad are actually produced with Cordyceps militaris. The main characteristic functional component in the fruiting ...

Claims

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Application Information

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IPC IPC(8): A01G1/04C12N1/14C12P19/40C12R1/645
Inventor 刘小莉黄开红周剑忠王英李莹单成俊张丽霞黄自苏
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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