X-chromosome specific molecular marker of Nile tilapia

A technology of Nile tilapia and X chromosome, which is applied in the direction of DNA/RNA fragments, microbial measurement/inspection, recombinant DNA technology, etc., can solve the problem of long distance, unsatisfactory sex-specific molecular markers, and inability to universally use Nile tilapia. Fish and other problems, to achieve the effect of increasing aquaculture production, reducing aquaculture costs, and improving economic benefits

Active Publication Date: 2013-11-27
SOUTHWEST UNIVERSITY
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Problems solved by technology

However, none of the above-mentioned sex-specific molecular markers is ideal, and the distance from the sex-determining locus of Nile tilapia is slightly fa...

Method used

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  • X-chromosome specific molecular marker of Nile tilapia
  • X-chromosome specific molecular marker of Nile tilapia
  • X-chromosome specific molecular marker of Nile tilapia

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Embodiment Construction

[0016] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0017] 1. Obtainment of sex-specific molecular markers in Nile tilapia

[0018] 1. Genomic DNA extraction

[0019] Cut Nile tilapia (the Japanese strain Nile tilapia introduced in this laboratory, its female parent is quoted from Egypt, and the male parent is quoted from Malaysia) fin rays 10mg, put lysate [10mM Tris-HCl (pH8. 0)+100mM EDTA(pH8.0)+100mM NaCl+5mg / ml SDS] homogenate in 600μl, add proteinase K with a final concentration of 20mg / ml and ribonuclease A (RnaseA) with a final concentration of 100...

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Abstract

The invention discloses an X-chromosome specific molecular marker of Nile tilapia. The nucleotide sequence of the X-chromosome specific molecular marker is as shown in SEQ ID No.3; the X-chromosome specific molecular marker can be commonly used for a plurality of strains of Nile tilapia; the genetic sex determination of the Nile tilapia can be performed economically, rapidly and accurately based on the molecular marker; the molecular marker is advantageous for establishing a method for producing YY supermale fish through mating of supermale fish YY with converted female fish YY; therefore, large-scale production of all male fish fry can be realized through mating of the YY supermale fish and the normal female fish XX; as a result, the yield of culture is improved remarkably, the culture cost is reduced and the economic benefit is enhanced.

Description

technical field [0001] The invention belongs to the technical field of molecular breeding and relates to a sex-specific molecular marker of tilapia. Background technique [0002] Tilapia, also known as African crucian carp, is native to the brackish waters of the African continent and the Atlantic coast of the Middle East. It has the advantages of low temperature, low oxygen resistance, adaptability and disease resistance. In recent decades, as countries around the world have successively introduced tilapia, it has become one of the main farmed fish species in the world. At present, the cultured species of tilapia in my country include Mozambique tilapia, Nile tilapia, Olia tilapia, etc., among which Nile tilapia is the main one. China is the largest producer and exporter of tilapia. [0003] Due to the short sexual cycle of tilapia, rapid reproduction, and slower growth of females than males, in order to avoid over-breeding of tilapia in farming and obtain commercial fis...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 王德寿孙运侣江东能黄宝锋曾圣叶凯
Owner SOUTHWEST UNIVERSITY
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