Y-chromosome specific molecular marker of Nile tilapia, and genetic sex determination method and supermale producing method both based on molecular marker

A Nile tilapia, molecular marker technology, applied in fish farming, DNA/RNA fragments, climate change adaptation, etc. problems, to achieve the effects of increasing breeding output, reducing breeding costs, and rapid and efficient production

CN103409420BActive Publication Date: 2015-04-15SOUTHWEST UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2015-04-15

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Abstract

The invention discloses a Y-chromosome specific molecular marker of Nile tilapia, wherein the nucleotide sequence of the Y-chromosome specific molecular marker is as shown in SEQ ID No.4; the Y-chromosome specific molecular marker can be commonly used for a plurality of strains of Nile tilapia; a genetic sex determination method of Nile tilapia is established based on the molecular marker, so that normal female fish XX, normal male fish XY, converted female fish XY, converted female fish YY and supermale fish YY can be differentiated from each other economically, rapidly and accurately; large-scale production of supermale fish fry can be realized through mating of YY supermale fish and the normal female fish XX based on the genetic sex determination method; as a result, the yield of culture is improved remarkably, the culture cost is reduced and the economic benefit is enhanced; besides, a method of producing the YY supermale fish through mating of the supermale fish YY and the converted female fish YY is also established by using the Y-chromosome specific molecular marker.
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Description

technical field

[0001] The invention belongs to the technical field of molecular breeding, and relates to a tilapia sex-specific molecular marker, a tilapia genetic sex identification method based on the molecular marker, and a method for producing super male fish based on the molecular marker. Background technique

[0002] Tilapia, also known as African crucian carp, is native to the brackish waters of the African continent and the Atlantic coast of the Middle East. It has the advantages of low temperature, low oxygen resistance, adaptability and disease resistance. In recent decades, as countries around the world have successively introduced tilapia, it has become one of the main farmed fish species in the world. At present, the cultured species of tilapia in my country include Mozambique tilapia, Nile tilapia, Olia tilapia, etc., among which Nile tilapia is the main species. China is the largest producer and exporter of tilapia.

[0003] Due to the short sexual cycle o...

Examples

Embodiment Construction

[0026] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0027] 1. Obtainment of sex-specific molecular markers in Nile tilapia

[0028] 1. Genomic DNA extraction

[0029]Cut Nile tilapia (the Japanese strain Nile tilapia introduced in this laboratory, its female parent is quoted from Egypt, and the male parent is quoted from Malaysia) fin rays 10mg, put lysate [10mM Tris-HCl (pH8. 0)+100mM EDTA(pH8.0)+100mM NaCl+5mg / ml SDS] homogenate in 600μl, add proteinase K with a final concentration of 20mg / ml and ribonuclease A (RnaseA) with a final concentration of 100μ...