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Specific molecular markers of sex chromosomes of Tilapia nilotica and genetic sex identification method

A Nile tilapia and molecular marker technology, applied in the field of genetic engineering, can solve the problems of inapplicability of genetic sex identification of Nile tilapia, undiscovered sex chromosome-specific molecular markers, and heavy workload of artificial breeding, etc., reaching The effect of reducing the cost of breeding, accelerating market demand and low cost

Active Publication Date: 2013-06-05
SOUTHWEST UNIV
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Problems solved by technology

[0004] The traditional production of all-male fish is mainly through artificial breeding, androgen-induced sex reversal, etc., but these methods have defects, such as artificial breeding workload, poor accuracy; androgen-induced sex reversal has food safety problems, etc.
In addition, there are the following research reports: Lee et al. used group segregation analysis to find 10 SSR markers linked to the sex of the tilapia phenotype. The identification of parent fish with Y chromosome copies; Ezaz et al. used AFLP to scan the Nile tilapia genome and found 3 Y chromosome linked AFLP markers (OniY425, OniY382 and OniY227) and 1 X chromosome linked AFLP marker (OniX420), but these Markers are not sex-specific and not suitable for genetic sexing of Nile tilapia
Therefore, so far, no sex chromosome-specific molecular markers that can be used for genetic sex identification of Nile tilapia have been found in the world

Method used

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  • Specific molecular markers of sex chromosomes of Tilapia nilotica and genetic sex identification method
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  • Specific molecular markers of sex chromosomes of Tilapia nilotica and genetic sex identification method

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Embodiment Construction

[0021] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method not indicating the specific conditions in the preferred embodiment is usually according to the conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang et al., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0022]1. Acquisition of sex chromosome-specific molecular markers in Nile tilapia

[0023] 1. Screening and cloning of Nile tilapia sex chromosome-specific AFLP markers

[0024] (1) Genomic DNA extraction

[0025] Cut 10 mg of Nile tilapia fin rays, put it into 600 μl of lysate [10mM Tris-HCl (pH8.0)+100mM EDTA (pH8.0)+100mM NaCl+5mg / ml SDS] for homogenization, and add the final concentration of 20mg / ml proteinase K and 100μg / ml final concentration of ribonuclease A (RNaseA), digested in a wat...

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Abstract

The invention belongs to the technical field of genetic engineering, in particular to specific molecular markers of sex chromosomes of Tilapia nilotica, wherein the nucleotide sequences of specific molecular markers NtY1 and NtY2 of a Y chromosome are respectively shown in SEQID No.1 and SEQID No.2, and the nucleotide sequence of a specific molecular marker NtX1 of a X chromosome is shown in SEQID No.3; in the invention, a genetic sex identification method of Tilapia nilotica is established by using the specific molecular markers of the sex chromosomes of the Nile tilapia, and through carrying out PCR amplification on any one or more than one of the specific molecular markers of the sex chromosomes, YY super-male fishes, XY milter and XX female fishes can be identified economically, quickly and accurately, thus being beneficial to realizing the large-scale production of holandric Tilapia nilotica fries through YY super-male fish breeding routes, obviously improving the breeding production of Tilapia nilotica, reducing the breeding cost, and improving the economic benefit.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to fish sex chromosome-specific molecular markers and a genetic sex identification method, in particular to tilapia sex chromosome-specific molecular markers and a genetic sex identification method. Background technique [0002] my country is rich in fish resources, many of which have gender differences in growth rate, and some species of females grow faster (40-100%) than males, such as flounder, semi-smooth tongue sole, star flounder, Southern catfish, etc.; some species of male individuals grow faster than female individuals (over 40%), such as yellow catfish, tilapia, etc. Carrying out research on genetic sex identification and sex control technology of these fish species has both important scientific significance and broad application prospects. [0003] Tilapia has excellent characteristics such as miscellaneous food, fast growth, strong reproduction, delicious taste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 王德寿孙运侣曾圣叶凯黄宝锋
Owner SOUTHWEST UNIV
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