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Specific molecular markers of sex chromosomes of silurus meridionalis and genetic sex identification method and unisexual fish production method based on molecular markers

A technology of molecular markers and chromosomal genes, applied in the determination/inspection of microorganisms, biochemical equipment and methods, fish farming, etc., can solve the problems of time-consuming and labor-intensive, so as to increase the production of aquaculture, reduce the cost of aquaculture, and improve the economy. benefit effect

Active Publication Date: 2019-12-10
SOUTHWEST UNIVERSITY
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Problems solved by technology

Traditional fish sex control breeding needs to judge the genotype of the parents through test crossing, which is time-consuming and labor-intensive. However, sex-linked molecular markers can easily and quickly realize the identification of genetic sex, which greatly saves manpower and material resources. However, in the south Specific molecular markers related to genetic sex have not been found in catfish

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  • Specific molecular markers of sex chromosomes of silurus meridionalis and genetic sex identification method and unisexual fish production method based on molecular markers
  • Specific molecular markers of sex chromosomes of silurus meridionalis and genetic sex identification method and unisexual fish production method based on molecular markers
  • Specific molecular markers of sex chromosomes of silurus meridionalis and genetic sex identification method and unisexual fish production method based on molecular markers

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Embodiment Construction

[0027] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0028] The present invention does not indicate the experimental method of concrete condition, generally according to routine condition, such as the condition described in molecular cloning experimental guideline (third edition, J. Sambrook et al., Huang Peitang et al. translation, Science Press, 2002) , or as recommended by the manufacturer.

[0029] 1. Obtainment of sex-specific molecular markers in catfish

[0030] 1. Genomic DNA extraction

[0031] Cut 10mg of southern catfish fin rays, put it into 600μl of lysate [10mM Tris-HCl(pH8.0)+100mM EDTA(pH8.0)+100mM NaCl+5mg / ml SDS] for homogenization, and add the final concentration to 20mg / ml Proteinase K and ribonuclease A (RnaseA) with a final concentration of 100 μg / ml were digested in a water bath at 55°C until clarified, and then 600 μl of phenol-chloroform-isoamyl alcohol (volume rati...

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Abstract

The invention relates to specific molecular markers of sexes of silurus meridionalis and a silurus meridionalis genetic sex identification method and a unisexual fish production method based on the molecular markers. The invention screens specific molecular markers of X and Y chromosomes of silurus meridionalis for the first time. A silurus meridionalis genetic sex PCR identification method established based on the molecular markers has the characteristics of high efficiency, accuracy and stability, can be applied to wild silurus meridionalis in laboratories and different watersheds, and can economically, quickly and accurately distinguish normal female fishes, normal male fishes, transformed female fishes, transformed male fishes and super-male fishes, thereby realizing large-scale production of complete female fish fries, significantly increasing the breeding yield, reducing the breeding cost, and improving economic benefits. Furthermore, a large number of unisexual fish fries can beobtained so as to be applied to fundamental research.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and relates to a sex-specific molecular marker for catfish, a genetic sex identification method for catfish based on the molecular marker and a production method for parthenomorphic fish. Background technique [0002] Fish sex determination, differentiation mechanism and sex control technology breeding are of great significance in aquaculture research and application. Many fishes are sexually dimorphic. ) and carp (Cyprinus carpio) and other females grow significantly faster than males, and the realization of all-feminization breeding through sex control technology is one of the key technologies to improve their breeding production (Chen Songlin et al., 2013; Bye and Lincoln, 1986; Shao et al. , 2014). On the contrary, males of Nile tilapia (Oreochromis niloticus), yellow catfish (Pelteobagrus fulvidraco) and channel catfish (Ictalurus punctatus) are significantly better than females in growth,...

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Application Information

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IPC IPC(8): C12Q1/6879A01K61/10C12N15/11
CPCA01K61/10C12Q1/6879C12Q2600/156Y02A40/81
Inventor 王德寿郑树清王晓双张帅龙娟
Owner SOUTHWEST UNIVERSITY
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