Method for simultaneously treating multiple kinds of biological tissue

A technology for simultaneous processing of biological tissues, applied in tissue regeneration, medical science, prostheses, etc., can solve the problems of low utilization rate of industrialized equipment, large damage to collagen fibers, and high toxicity of disinfectant solutions, and achieve high resistance to degradation, The effect of uniform processing degree and large amount of biological tissue

Active Publication Date: 2017-01-04
SUZHOU HENRUI DISHENG MEDICAL CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (2) Use benzalkonium bromide solution for disinfection, NaCl or EDTA hypertonic solution and detergent Tween, SDS or Triton for decellularization, and finally ethylene oxide sterilization after washing with phosphate buffer, the disinfectant used in this method The toxicity is high, the decellularization reagent used is difficult to clean, and the damage to collagen fibers is also large;
[0009] (5) A single batch can only process a single type of biological tissue, with low efficiency and low utilization rate of industrialized equipment

Method used

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  • Method for simultaneously treating multiple kinds of biological tissue
  • Method for simultaneously treating multiple kinds of biological tissue
  • Method for simultaneously treating multiple kinds of biological tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Step 1. Preprocessing

[0070] Wash the pig small intestine, bovine pericardium, and pig skin with purified water, and peel off useless tissues such as the mesentery and serosa layer of the pig small intestine, the fat particles adhered to the surface of the bovine pericardium, the epidermis of pig skin and the subcutaneous fat layer, etc., to obtain the submucosa of pig small intestine, Bovine pericardium and porcine dermis were sterilized with 75% ethanol at room temperature for 0.5h.

[0071] Step two, degreasing

[0072] Soak all kinds of tissues that have been pretreated in step 1 in isopropanol at room temperature for 48 hours, and then rinse with purified water 8 times to remove isopropanol.

[0073] Step 3. Physical decellularization

[0074] Put all kinds of tissues degreased in step 2 in ultra-high pressure equipment, treat them at 10MPa and 4°C for 60 minutes, then soak them in 50 U / mL DNase solution at room temperature at 100 rpm for 24 hours, and use 50 U...

Embodiment 2

[0082] Step 1. Preprocessing

[0083] Wash the pig small intestine, bovine pericardium, and pig skin with purified water, and peel off useless tissues such as the mesentery and serosa layer of the pig small intestine, the fat particles adhered to the surface of the bovine pericardium, the epidermis of pig skin and the subcutaneous fat layer, etc., to obtain the submucosa of pig small intestine, Bovine pericardium and porcine dermis were sterilized with 2% hydrogen peroxide for 1 hour at room temperature.

[0084] Step two, degreasing

[0085] Soak various tissues that have been pretreated in Step 1 in 1% sodium deoxycholate at room temperature for 12 hours, and then rinse with purified water for 3 times to remove sodium deoxycholate.

[0086] Step 3. Physical decellularization

[0087] Put all kinds of tissues degreased in step 2 into ultra-high pressure equipment, treat them at 5MPa and 40°C for 1min, then shake and soak them with 30U / mL DNase solution at room temperature a...

Embodiment 3

[0095] Step 1. Preprocessing

[0096] Wash the pig small intestine, bovine pericardium, and pig skin with purified water, and peel off useless tissues such as the mesentery and serosa layer of the pig small intestine, the fat particles adhered to the surface of the bovine pericardium, the epidermis of pig skin and the subcutaneous fat layer, etc., to obtain the submucosa of pig small intestine, Bovine pericardium and pig dermis were sterilized with 75% ethanol for 2 hours.

[0097] Step two, degreasing

[0098]Soak all kinds of tissues that have been pretreated in Step 1 in 3% sodium deoxycholate at room temperature for 24 hours, and then rinse with purified water for 4 times to remove sodium deoxycholate.

[0099] Step 3. Physical decellularization

[0100] Put all kinds of tissues degreased in step 2 into ultra-high pressure equipment, treat them at 1MPa and 25°C for 30min, then soak them with 1U / mL DNase solution at 150rpm at room temperature for 4h, and 1U / L α-Gal enzyme...

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Abstract

The invention discloses a method for simultaneously treating multiple kinds of biological tissue. The method includes the steps of pretreating, degreasing, cell extraction, crosslinking and sterilization. The method for simultaneously treating the multiple kinds of biological tissue can simultaneously treat the multiple kinds of biological tissue, the batch-treated biological tissue amount is large, the treatment degree is uniform, the process is simple, industrialization is easy, and efficient production is achieved. According to the method for simultaneously treating the multiple kinds of biological tissue, use of strong acid, strong base and protease is completely avoided, natural three-dimensional collagen support fiber structures of extracellular matrixes of the multiple kinds of biological tissue can be reserved, and cell components, causing immunogenicity, in the tissue are removed. The biological tissue prepared through the method has good bio-compatibility and mechanical performance, compared with natural biological tissue, high degradation resistance is achieved, and the biological tissue can be used for repairing various kinds of soft tissue injuries.

Description

technical field [0001] The invention relates to the technical field of tissue engineering medical biomaterials, in particular to a method for simultaneously processing multiple biological tissues. Background technique [0002] As the aging society becomes more and more serious, the incidence of cancer also increases. After the resection of various solid tumors, the soft tissue around the tumor tissue is often damaged or weakened. For example, after breast cancer resection, the soft tissue of the chest wall is weakened. When breast reconstruction is performed, complications such as prosthetic displacement and protrusion are often easily caused. [0003] In view of the above problems, the use of allogeneic acellular dermal matrix and heterogeneous acellular matrix for soft tissue repair after tumor resection has a better effect, and has been clinically used for more than 20 years at home and abroad. This kind of acellular matrix products can also be used for the reinforcement...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3683A61L27/50A61L2430/40
Inventor 刘博文周宁辉俞鸿飞朱鑫建王志东
Owner SUZHOU HENRUI DISHENG MEDICAL CO LTD
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