Biodetection articles

A technology for products and detection reagents, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, and methods of sampling biological materials, etc., and can solve problems such as the complexity of living cell detection.

Inactive Publication Date: 2011-11-16
3M INNOVATIVE PROPERTIES CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The presence of extracellular material (such as acellular ATP released into the environment from dead or stressed animal cells, textile cells, and / or The detection of cells is complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0138] Incorporation of cell extractants into hydrogel beads during hydrogel polymerization .

[0139] Beads were prepared as described in Example 1 of International Patent Publication No. WO 2007 / 146722, where deionized water was replaced with the desired loading solution. By mixing 40 grams of 20 mole ethoxylated trimethylolpropane triacrylate (EO 20 - TMPTA) (SR415, available from Sartomer, Exeter, PA), 60 grams of deionized (DI) water, and 0.8 grams of photoinitiator (IRGACURE 2959, available from Ciba Specialty Chemicals, Tarrytown, NY) to prepare a uniform precursor combination thing. The precursor composition was poured into the funnel so that the precursor composition exited the funnel through a 2.0 mm diameter hole. The precursor composition was dropped along a vertical axis of a 0.91 m long, 51 mm diameter quartz tube extending through a UV exposure zone consisting of a light screen and a 240 W / cm irradiator equipped with a 25 cm long "H" bulb (available from Fu...

preparation example 2

[0143] Incorporation of cationic monomers into hydrogel beads during hydrogel polymerization .

[0144] Polymer beads with cationic monomers were prepared as described in Examples 30-34 of International Patent WO2007 / 146722. The precursor compositions used to prepare the beads are shown in Table 1. The components of the precursor composition were stirred together in a brown jar until the antimicrobial monomer dissolved.

[0145] Formation of DMAEMA-C in a three-necked round-bottomed reaction flask equipped with a mechanical stirrer, temperature probe, and condenser 8 Br. A reaction flask was charged with 234 parts dimethylaminoethyl methacrylate, 617 parts acetone, 500 parts 1-bromoethane, and 0.5 parts BHT antioxidant. The mixture was stirred at 35°C for 24 hours. At this point the reaction mixture was cooled to room temperature to give a clear slightly yellow solution. The solution was transferred to a round bottom flask and the acetone was removed by rotary evaporati...

preparation example 3

[0151] Incorporation of luciferin into hydrogel beads during hydrogel polymerization .

[0152] Luciferin-containing hydrogel beads were similarly prepared as follows: 20 parts of EO 20 - TMPTA was mixed with 30 parts of luciferin (2 mg in 30 ml of 14 mM phosphate buffer (pH 6.4)) and 0.4 part of photoinitiator (IRGACURE 2959) and exposed to ultraviolet light to prepare beads. The beads were then stored in jars at 4°C and labeled luciferin-1s.

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Abstract

Articles are provided for the detection of cells in a sample (610). The articles include a hydrogel (640) comprising a cell extractant. Methods of use are also disclosed.

Description

[0001] Cross references to related patent applications [0002] This application claims the benefit of US Provisional Application Serial Nos. 61 / 101,546 and 61 / 101,563, both filed September 30,2008. Background technique [0003] Various assays are available that can be used to assess the presence of biological analytes in samples (eg, surfaces, water, air, etc.). Such assays include assays based on the detection of ATP using the firefly luciferase reaction, assays based on the detection of proteins using colorimetric methods, assays based on the detection of microorganisms using microbial culture techniques, and assays based on the detection of microorganisms using immunochemical techniques. The surface can be sampled using a swab device or by direct contact with a culture device such as an agar plate. Samples can be analyzed for the presence of living cells, particularly living microorganisms. [0004] The results obtained from this type of test are usually used to judge t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12M1/30
CPCB01L2400/0683C12Q1/485G01N2001/028C12Q1/66B01L3/5029B01L2300/0672B01L2200/16B01L2300/087C12Q1/04B01L3/502
Inventor 拉伊·拉贾戈帕尔马修·D·雷耶尔罗宾·E·赖特卡罗琳·M·伊利塔洛
Owner 3M INNOVATIVE PROPERTIES CO
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