Lactobacillus paraplantarum capable of reducing cholesterol and nitrite and screening method thereof

A type of Lactobacillus plantarum and cholesterol-lowering technology, applied in the field of microbiology, can solve the problems of no Lactobacillus plantarum and achieve the effects of improving the body's immunity, improving the intestinal flora, and reducing the residual amount

Active Publication Date: 2016-06-22
北京中京丰创科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

These Lactobacillus plantarum have a certain cholesterol-lowering function, but so far, there is no applic

Method used

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  • Lactobacillus paraplantarum capable of reducing cholesterol and nitrite and screening method thereof
  • Lactobacillus paraplantarum capable of reducing cholesterol and nitrite and screening method thereof
  • Lactobacillus paraplantarum capable of reducing cholesterol and nitrite and screening method thereof

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Experimental program
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Embodiment Construction

[0025] Example screening Lactobacillus plantarum GUFHSL70 bacterial strain:

[0026] 1. Sample collection and strain isolation and purification

[0027] Samples were collected from Maocai and Maotang in Qiannan area, Guizhou Province. Take 10g or 10mL of the sample and put it into 90mL of sterile peptone water, shake it on the shaker for 30min, and take 200μL of the supernatant in the ultra-clean workbench and spread it on CaCO3- On the MRS agar plate, culture in a carbon dioxide incubator at 37°C for 48 hours, select a single colony that produces an obvious calcium dissolution circle for further purification, and then store it on the slant for future use.

[0028] 2. Colony morphology identification, physiological and biochemical identification and molecular biological identification

[0029] 2.1 The strain was cultured on the MRS plate for 24 hours, and the colony shape and color were observed and recorded.

[0030] 2.2 Gram staining: pick the colonies on the plate for sme...

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Abstract

The invention provides a lactobacillus paraplantarum capable of reducing cholesterol and nitrite. The lactobacillus paraplantarum is separated from traditional fermented preserved vegetables of minority nationalities in Guizhou, and is named as Lactobacillus paraplantarum GUFHSL-70, and lactobacillus paraplantarum pure culture is collected by CCTCC (China Center For Type Culture Collection) with the collection number of CCTCC M 2016002 in Wuhan University, Wuhan, China on Jan. 1 2016. The lactobacillus paraplantarum GUFHSL-70 is functional lactobacillus which has favorable characteristic in the aspect of reducing cholesterol and nitrite; through developing the lactobacillus paraplantarum GUFHSL-70 into health foods or medicine and taking the health foods or medicine for a long term, intestinal flora can be improved, serum cholesterol is reduced, and the immunity of the organism is improved; or by virtue of adding the lactobacillus paraplantarum GUFHSL-70 into the fermented preserved vegetables, the amount of nitrate residues in the preserved vegetables is reduced, the fermentation period is shortened, and the product flavor is improved.

Description

technical field [0001] The present invention relates to microorganisms, more specifically to lactic acid bacteria, in particular to lactic acid bacteria with both cholesterol-lowering ability and nitrite-degrading ability. Background technique [0002] According to medical research, elevated cholesterol levels in serum are considered to be an important risk factor for cardiovascular diseases such as coronary heart disease and atherosclerosis. The World Health Organization (WHO) predicts that cardiovascular disease will remain the leading cause of death until 2030, affecting 23 million people. Clinical studies have shown that exceeding the normal serum cholesterol level (>5.2mmol) by 1mmol increases the risk of coronary heart disease by 35%. At the same time, reducing the serum cholesterol level by 1% will reduce the risk of disease by 2% to 3%. [0003] Studies have found that acute nitrite poisoning leads to methemoglobinemia, and chronic poisoning has the risk of carci...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225
CPCC12N1/20C12N1/205C12R2001/225
Inventor 何腊平宋小娟李翠芹
Owner 北京中京丰创科技有限公司
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